Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,025,579	T→C	S23S (AGT→AGC)	yedP →	putative mannosyl‑3‑phosphoglycerate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,025,579	0	T	C	100.0%	155.3 / NA	44	S23S (AGT→AGC)	yedP	putative mannosyl‑3‑phosphoglycerate phosphatase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (31/13); total (31/13)

CGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTG  >  NC_000913/2025442‑2025714

cGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTa                                                                                                                                       >  1:471756/1‑140 (MQ=255)

    aaaaTCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGAc                                                                                                                                   <  2:471756/140‑1 (MQ=255)

                 aTAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAg                                                                                                                      >  2:799017/1‑140 (MQ=255)

                         tgCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTgg                                                                                                              >  1:710603/1‑140 (MQ=255)

                             gTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCa                                                                                                          <  1:652847/140‑1 (MQ=255)

                                     aaTCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTa                                                                                                  <  1:799017/140‑1 (MQ=255)

                                        catcCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTAcgc                                                                                               >  1:491102/1‑140 (MQ=255)

                                        catcCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTAcgc                                                                                               >  1:715346/1‑140 (MQ=255)

                                                      gagTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTcc                                                                                 >  2:65637/1‑140 (MQ=255)

                                                         tACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGt                                                                              >  2:407933/1‑140 (MQ=255)

                                                         tACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGt                                                                              >  2:544642/1‑140 (MQ=255)

                                                              cgcgACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCa                                                                            >  2:652624/1‑137 (MQ=255)

                                                              cgcgACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCa                                                                            <  1:652624/137‑1 (MQ=255)

                                                              cgcgACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATtc                                                                         >  2:511298/1‑140 (MQ=255)

                                                                  aCTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCCATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCAtt                                                                          <  1:268165/135‑1 (MQ=255)

                                                                  aCTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCCATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCAtt                                                                          >  2:268165/1‑135 (MQ=255)

                                                                                  aacaacCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCa                                                     >  2:143770/1‑140 (MQ=255)

                                                                                   acaacCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAg                                                    <  1:603103/140‑1 (MQ=255)

                                                                                    caacCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGc                                                   >  1:488016/1‑140 (MQ=255)

                                                                                         actactGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGaaa                                              >  1:649370/1‑140 (MQ=255)

                                                                                                 tttttAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTAc                                      >  1:589612/1‑140 (MQ=255)

                                                                                                  ttttAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAACAGTAAAACATCAGCGGAAATGCTGTACt                                     >  1:858418/1‑140 (MQ=255)

                                                                                                   tttAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACtt                                    >  2:104054/1‑140 (MQ=255)

                                                                                                   tttAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACtt                                    >  1:184566/1‑140 (MQ=255)

                                                                                                                 tGGCACCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTgg                      <  2:766492/140‑1 (MQ=255)

                                                                                                                     aCCCTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCt                  >  1:118484/1‑140 (MQ=255)

                                                                                                                     aCCCTGCTGGACAGTCATAGCTATGACTGGCAACC‑GCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTa                 >  2:477209/1‑140 (MQ=255)

                                                                                                                       ccTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTgg                      >  2:219900/1‑134 (MQ=255)

                                                                                                                       ccTGCTGGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTgg                      <  1:219900/134‑1 (MQ=255)

                                                                                                                        ctgctgGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACa               <  2:184566/140‑1 (MQ=255)

                                                                                                                         tgctgGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTgggg                    >  2:693232/1‑134 (MQ=255)

                                                                                                                         tgctgGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTgggg                    <  1:693232/134‑1 (MQ=255)

                                                                                                                         tgctgGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACaa              >  2:846182/1‑140 (MQ=255)

                                                                                                                         tgctgGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACaa              >  2:242237/1‑140 (MQ=255)

                                                                                                                         tgctgGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACa               <  2:508865/139‑1 (MQ=255)

                                                                                                                         tgctgGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACa               >  1:508865/1‑139 (MQ=255)

                                                                                                                          gctgGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAg             >  1:540527/1‑140 (MQ=255)

                                                                                                                          gctgGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAg             <  2:118484/140‑1 (MQ=255)

                                                                                                                           ctgGACAGTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAgg            >  1:309954/1‑140 (MQ=255)

                                                                                                                                  gTCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCg     >  2:674559/1‑140 (MQ=255)

                                                                                                                                   tCATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGc    >  1:772514/1‑140 (MQ=255)

                                                                                                                                    cATAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCt   <  1:104054/140‑1 (MQ=255)

                                                                                                                                     aTAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTg  >  2:550811/1‑140 (MQ=255)

                                                                                                                                     aTAGCTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTg  >  1:38538/1‑140 (MQ=255)

CGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTG  >  NC_000913/2025442‑2025714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A