Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F28 I1 R1
|
227 |
52.0 |
1764248 |
99.3% |
1751898 |
138.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,165,573 |
(C)6→7 |
intergenic (+52/‑95) |
yegP → / → yegQ |
DUF1508 domain‑containing protein YegP/putative peptidase YegQ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,165,567 | 1 | . | C | 96.9%
| 107.6
/ ‑3.1
| 32 | intergenic (+46/‑101) | yegP/yegQ | DUF1508 domain‑containing protein YegP/putative peptidase YegQ |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (14/17); total (14/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑G‑CCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGA > NC_000913/2165435‑2165700
|
aTGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑G‑ccccccc > 2:702835/1‑139 (MQ=255)
tGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑G‑ccccccca < 1:171292/140‑3 (MQ=255)
tGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑G‑ccccccca > 2:171292/1‑138 (MQ=255)
gCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAgcg < 1:725270/140‑1 (MQ=255)
ccGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTAc > 2:503588/1‑140 (MQ=255)
cgcgCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACa > 1:783865/1‑140 (MQ=255)
cgcAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAAt > 2:376661/1‑140 (MQ=255)
aaTCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATgcc < 2:783865/140‑1 (MQ=255)
tctcGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATgcccg < 2:710268/140‑1 (MQ=255)
gAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTa > 2:341232/1‑140 (MQ=255)
ccGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGt > 2:211669/1‑140 (MQ=255)
ttGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑G‑CCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCa < 2:140425/140‑1 (MQ=255)
tGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGCGCTGTTGGCCTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGCGCa < 2:876041/140‑1 (MQ=255)
gTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTggggg > 2:856426/1‑131 (MQ=255)
gTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTggggg < 1:856426/131‑1 (MQ=255)
aaGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAAcc < 1:376661/140‑1 (MQ=255)
aaaggCCCAAGCCAGACGGTGAAAGACAATACGTAATTCCGATGACGGGCGTGGGTTGTTGGGGTCCGGCGCTTTACCAGACC‑CCCCCCACAGCGCTACAACCCCCGCCCTTGAAGTGGGGGCACTCCCCTAACCGCTtc < 2:53971/137‑1 (MQ=255)
aTGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTtca > 1:278669/1‑140 (MQ=255)
ccAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAg < 2:30734/140‑1 (MQ=255)
aCGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGa > 1:398189/1‑140 (MQ=255)
aaaGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGAc > 2:114242/1‑140 (MQ=255)
aaaGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGAc < 2:398189/140‑1 (MQ=255)
gACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTg < 1:503588/140‑1 (MQ=255)
aTACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGCGAAGCGGATCTGACCTGtcat > 1:96650/1‑140 (MQ=255)
cGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAg < 1:341232/140‑1 (MQ=255)
ttACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACga < 2:308599/140‑1 (MQ=255)
tGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAAt > 1:496099/1‑140 (MQ=255)
gCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGCGAAGCGGATCTGACCTGTCATCAGAACGAGAGAAtt < 2:96650/140‑1 (MQ=255)
cGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTAt > 2:580127/1‑140 (MQ=255)
gCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGtt < 1:114242/140‑1 (MQ=255)
gggTTGTTGGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTaaa > 2:718981/1‑140 (MQ=255)
tgttgGCGTCCGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCgg < 1:580127/140‑1 (MQ=255)
cGGCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTc > 1:286348/1‑140 (MQ=255)
ggCGCTTTACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTcc < 1:718981/140‑1 (MQ=255)
tACCAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCcggcgg < 2:278669/140‑1 (MQ=255)
ccAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGa < 1:468467/140‑1 (MQ=255)
ccAGA‑GCCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGa < 1:702835/140‑1 (MQ=255)
|
ATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA‑G‑CCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGA > NC_000913/2165435‑2165700
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
