Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,237,065	T→C	N230S (AAC→AGC)	mglC ←	D‑galactose/methyl‑galactoside ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,237,065	0	T	C	100.0%	166.1 / NA	49	N230S (AAC→AGC)	mglC	D‑galactose/methyl‑galactoside ABC transporter membrane subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/23); total (26/23)

CGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGG  >  NC_000913/2236928‑2237194

cGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTc                                                                                                                                 >  1:798038/1‑140 (MQ=255)

            acgcCGCGATAGCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCGTAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTg                                                                                                                     >  1:480537/1‑140 (MQ=255)

             cgccgcGATAGCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGa                                                                                                                    >  1:107021/1‑140 (MQ=255)

               ccgcGATAGCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGaca                                                                                                                  <  2:722838/140‑1 (MQ=255)

                      aGCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATa                                                                                                           <  2:503639/140‑1 (MQ=255)

                            cAGCTCATACATAAAGCCGAGGTTGTTGGTGGCCGCGCCGATCCGCCCGGCTCCTCACATCCCGCCACCCGCGTAGACCACGCCAGACAACGCGTCGCTCAGCCGCTGGTTCAGGCCGTCGTTGTCACCAGATACGTTTg                                                                                                     <  2:559547/140‑1 (MQ=255)

                                    acataAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTcc                                                                                             <  2:809712/140‑1 (MQ=255)

                                         aaGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGtt                                                                                        >  2:469960/1‑140 (MQ=255)

                                          aGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTa                                                                                       >  1:668176/1‑140 (MQ=255)

                                          aGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTa                                                                                       >  1:694266/1‑140 (MQ=255)

                                              gAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGc                                                                                   >  2:45137/1‑140 (MQ=255)

                                               aGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGcc                                                                                  >  2:576103/1‑140 (MQ=255)

                                                      tggtggCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACCCGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCa                                                                           <  2:726870/140‑1 (MQ=255)

                                                          ggCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCaaaaa                                                                       >  1:869618/1‑140 (MQ=255)

                                                            cAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATg                                                                     >  2:452216/1‑140 (MQ=255)

                                                               agCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGTCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTc                                                                  >  1:689012/1‑140 (MQ=255)

                                                               agCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTc                                                                  >  1:838591/1‑140 (MQ=255)

                                                                       cGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGaa                                                          <  1:152366/140‑1 (MQ=255)

                                                                              cTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGtt                                                   >  1:707967/1‑140 (MQ=255)

                                                                              cTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGtt                                                   <  2:798038/140‑1 (MQ=255)

                                                                              cTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTCGCCGCTTCCGGGTTCCCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGtt                                                   <  2:869618/140‑1 (MQ=255)

                                                                                  tAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGt                                               >  1:844092/1‑140 (MQ=255)

                                                                                         ccGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCacaa                                        >  2:137156/1‑140 (MQ=255)

                                                                                          cGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCacaac                                       >  1:385181/1‑140 (MQ=255)

                                                                                          cGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCacaac                                       >  1:206360/1‑140 (MQ=255)

                                                                                                     tAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGaa                            >  2:379173/1‑140 (MQ=255)

                                                                                                     tAGAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGaa                            >  2:197697/1‑140 (MQ=255)

                                                                                                       gAACACGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACg                          >  1:89688/1‑140 (MQ=255)

                                                                                                           acGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCAc                      <  2:129801/140‑1 (MQ=255)

                                                                                                            cGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACg                          >  2:358788/1‑135 (MQ=255)

                                                                                                            cGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACg                          <  1:358788/135‑1 (MQ=255)

                                                                                                            cGCCAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCAcc                     >  2:287740/1‑140 (MQ=255)

                                                                                                              ccAGACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATCCTTTTGCCGCTGCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGc                   <  1:100241/140‑1 (MQ=255)

                                                                                                                 gACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAAt                <  1:137156/140‑1 (MQ=255)

                                                                                                                 gACAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAAt                <  2:668176/140‑1 (MQ=255)

                                                                                                                  aCAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATc               <  2:107021/140‑1 (MQ=255)

                                                                                                                  aCAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATc               >  1:70905/1‑140 (MQ=255)

                                                                                                                  aCAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATc               <  1:689754/140‑1 (MQ=255)

                                                                                                                  aCAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATc               >  2:689754/1‑140 (MQ=255)

                                                                                                                  aCAACGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATc               >  1:399212/1‑140 (MQ=255)

                                                                                                                     aCGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGTCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAAc            <  2:689012/140‑1 (MQ=255)

                                                                                                                     aCGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAAc            <  1:828096/140‑1 (MQ=255)

                                                                                                                     aCGCGTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAAc            <  2:504479/140‑1 (MQ=255)

                                                                                                                      cgcgTAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAAcg           >  1:88934/1‑140 (MQ=255)

                                                                                                                          tAGATCATCAGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTa       <  1:287740/140‑1 (MQ=255)

                                                                                                                              tcatcaGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAg   <  2:707967/140‑1 (MQ=255)

                                                                                                                               catcaGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAgg  <  1:469960/140‑1 (MQ=255)

                                                                                                                               catcaGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAgg  <  1:576103/140‑1 (MQ=255)

                                                                                                                               catcaGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAgg  <  2:454341/140‑1 (MQ=255)

                                                                                                                               catcaGCAGGCTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAgg  <  2:385181/140‑1 (MQ=255)

CGCCTACCACGCACGCCGCGATAGCATCCAGCTCATACATAAAGCCGAGGTTGTTGGTGGCAGAGCCGATACGTCCGGCTTCTAACATCCCGCCAAAGGCATAGAACACGCCAGACAACGCGTAGATCATCAGCAGGTTCAGGCCGACGTTGACACCAGATACTTTTGCCGCTTCCGGGTTACCGCCAATGGCAAAAATGTTCTTACCGAAGCGGGTTTTGTTCCACAACACCCAGACGAACGCCACCGCAATCAACGCGTAGAAGG  >  NC_000913/2236928‑2237194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A