Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 227 52.0 1764248 99.3% 1751898 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,309,762 A→G L215P (CTG→CCG)  ada ← DNA‑binding transcriptional dual regulator/DNA repair protein Ada

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,309,7620AG100.0% 47.3 / NA 15L215P (CTG→CCG) adaDNA‑binding transcriptional dual regulator/DNA repair protein Ada
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/6);  total (9/6)

AGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGT  >  NC_000913/2309627‑2309892
                                                                                                                                       |                                                                                                                                  
aGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCGGCaa                                                                                                                                >  2:457299/1‑140 (MQ=255)
               tgattgaGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCGGCAATATCGCGCAAATccc                                                                                                                 >  2:422485/1‑140 (MQ=255)
                       gCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCGGCAATATCGCGCAAATCCCCcgctcgc                                                                                                         >  2:797773/1‑140 (MQ=255)
                                       cGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCGGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCa                                                                                         <  1:457299/140‑1 (MQ=255)
                                                  cTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCGGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCAACGACCCAGCt                                                                              <  1:422485/140‑1 (MQ=255)
                                                      aaCATCAGATCGGCAGGCGCGTTGTCGGCCGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCGGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCAACGACCCAGCTcaca                                                                          >  1:2471/1‑140 (MQ=255)
                                                        cATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCGGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCAACGACCCAGCTca                                                                            >  1:304812/1‑136 (MQ=255)
                                                        cATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCGGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCAACGACCCAGCTca                                                                            <  2:304812/136‑1 (MQ=255)
                                                                                        gAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCGGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCg                                        >  2:432203/1‑140 (MQ=255)
                                                                                                      aaCTCGCTGATTAGTGTCGCGTCATCATCGCCCGGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGaa                          <  1:432203/140‑1 (MQ=255)
                                                                                                      aaCTCGCTGATTAGTGTCGCGTCATCATCGCCCGGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGaa                          >  1:457774/1‑140 (MQ=255)
                                                                                                       aCTCGCTGATTAGTGTCGCGTCATCATCGCCCGGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAAt                         >  1:581186/1‑140 (MQ=255)
                                                                                                                  aGTGTCGCGTCATCATCGCCCGGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGt              <  2:581186/140‑1 (MQ=255)
                                                                                                                        gcgTCATCATCGCCCGGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGcc        <  2:457774/140‑1 (MQ=255)
                                                                                                                              tcatcGCCCGGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGt  >  2:124892/1‑140 (MQ=255)
                                                                                                                              tcatcGCCCGGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGt  >  2:585394/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                  
AGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGATTAGTGTCGCGTCATCATCGCCCAGCAATATCGCGCAAATCCCCCGCTCGCTTTCTGCCACCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGT  >  NC_000913/2309627‑2309892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A