Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,578,847	(A)_8→9	coding (182/951 nt)	talA →	transaldolase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,578,839	1	.	A	97.6%	143.8 / ‑3.2	42	coding (174/951 nt)	talA	transaldolase A
Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (23/18); total (23/19)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.77e-01

TCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGT‑AAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAATCGTACCCGGTCGCGTGTCAACAGAAGTTGATGCACGCCTCTCTTTTG  >  NC_000913/2578703‑2578972

tCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGT‑aaa                                                                                                                                    >  2:516111/1‑140 (MQ=255)

tCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGT‑aaa                                                                                                                                    >  1:643587/1‑140 (MQ=255)

   tGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGT‑aaaaaa                                                                                                                                 >  1:204582/1‑140 (MQ=255)

      cAGACAGCGGCGATATTGAGTCCATTCGCCTTTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGT‑aaaaaaaaa                                                                                                                              <  2:215307/140‑2 (MQ=255)

          cAGCGGCGATATTGAGTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGT‑aaaaaaaaatggc                                                                                                                          >  1:365094/1‑135 (MQ=255)

             cggcgATATTGAGTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAg                                                                                                                        >  2:455981/1‑140 (MQ=255)

                cgATATTGAGTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGAc                                                                                                                      <  2:8698/139‑1 (MQ=255)

                cgATATTGAGTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGAc                                                                                                                      >  1:8698/1‑139 (MQ=255)

                 gATATTGAGTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGAccc                                                                                                                    <  1:873707/140‑1 (MQ=255)

                 gATACTGAGTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGAccc                                                                                                                    <  2:814120/140‑1 (MQ=255)

                    atTGAGTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAgg                                                                                                                 <  1:649055/140‑1 (MQ=255)

                         gTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGaacaa                                                                                                            <  1:623673/140‑1 (MQ=255)

                            cATTCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGT‑AAAAAAAATGGCAAGACCCAGGAACAACAgg                                                                                                        <  1:130042/140‑1 (MQ=255)

                             aTTCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGAccc                                                                                                                    >  2:748576/1‑128 (MQ=255)

                             aTTCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGAccc                                                                                                                    <  1:748576/128‑1 (MQ=255)

                             aTTCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAgg                                                                                                        <  1:435318/140‑1 (MQ=255)

                             aTTCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAgg                                                                                                        >  1:106943/1‑140 (MQ=255)

                              ttCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAggt                                                                                                       >  1:699024/1‑140 (MQ=255)

                                   cATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCg                                                                                                  <  1:516111/140‑1 (MQ=255)

                                          atcCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCgtg                                                                                           <  1:645124/140‑1 (MQ=255)

                                           tcCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCgtgt                                                                                          >  2:347361/1‑140 (MQ=255)

                                            ccccAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGtgtg                                                                                         >  2:132726/1‑140 (MQ=255)

                                                       caccaccaATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCg                                                                              >  2:745735/1‑140 (MQ=255)

                                                               aTCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAAttt                                                                      <  1:455981/140‑1 (MQ=255)

                                                               aTCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAAttt                                                                      <  1:132726/140‑1 (MQ=255)

                                                                     cGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGc                                                                >  2:792841/1‑140 (MQ=255)

                                                                          ttACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGaaa                                                           >  2:23883/1‑140 (MQ=255)

                                                                                    cTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGaa                                                            <  2:319672/129‑1 (MQ=255)

                                                                                    cTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGaa                                                            >  1:319672/1‑129 (MQ=255)

                                                                                    cTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAAt                                                 >  1:467801/1‑140 (MQ=255)

                                                                                    cTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAAt                                                 >  2:185783/1‑140 (MQ=255)

                                                                                       ccGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAATCga                                              >  2:32264/1‑139 (MQ=255)

                                                                                                cacaATATGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAATCGTACCCGGTcg                                     <  1:347361/140‑1 (MQ=255)

                                                                                                     tatGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAATCGTACCCGGt                                       <  2:172525/133‑1 (MQ=255)

                                                                                                     tatGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAATCGTACCCGGt                                       >  1:172525/1‑133 (MQ=255)

                                                                                                     tatGAGCATTTAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAATCGTACCCGGTCGCgtgt                                >  2:622787/1‑140 (MQ=255)

                                                                                                             tttAATAGACGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAATCGTACCCGGTCGCGTGTCAACAGaa                        >  1:610812/1‑140 (MQ=255)

                                                                                                                     aCGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAATCGTACCCGGTCGCGTGTCAACAGAAGTTGATGc                >  1:448311/1‑140 (MQ=255)

                                                                                                                      cGATGCTATCGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAATCGTACCCGGTCGCGTGTCAACAGAAGTTGATGCa               <  1:792841/140‑1 (MQ=255)

                                                                                                                               cGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAATCGTACCCGGTCGCGTGTCAACAGAAGTTGATGCa               >  2:112673/1‑131 (MQ=255)

                                                                                                                               cGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAATCGTACCCGGTCGCGTGTCAACAGAAGTTGATGCa               <  1:112673/131‑1 (MQ=255)

                                                                                                                               cGCCTGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAATCGTACCCGGTCGCGTGTCAACAGAAGTTGATGCACGCctctct      <  2:365094/140‑1 (MQ=255)

                                                                                                                                   tGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAATCGTACCCGGTCGCGTGTCAACAGAAGTTGATGCACGCCTCTCTTTTg  <  2:204582/140‑1 (MQ=255)

                                                                                                                                   tGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAATCGTACCCGGTCGCGTGTCAACAGAAGTTGATGCACGCCTCTCTTTTg  >  2:717969/1‑140 (MQ=255)

                                                                                                                                   tGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAATCGTACCCGGTCGCGTGTCAACAGAAGTTGATGCACGCCTCTCTTTTg  >  1:853605/1‑140 (MQ=255)

                                                                                                                                   tGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAATCGTACCCGGTCGCGTGTCAACAGAAGTTGATGCACGCCTCTCTTTTg  >  1:730895/1‑140 (MQ=255)

                                                                                                                                   tGGGGTAAAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAATCGTACCCGGTCGCGTGTCAACAGAAGTTGATGCACGCCTCTCTTTTg  >  1:63091/1‑140 (MQ=255)

TCGTGGCAGACAGCGGCGATATTGAGTCCATTCGCCATTATCATCCCCAGGATGCCACCACCAATCCTTCGCTGTTACTCAAAGCTGCCGGATTATCACAATATGAGCATTTAATAGACGATGCTATCGCCTGGGGT‑AAAAAAAATGGCAAGACCCAGGAACAACAGGTGGTCGCAGCGTGTGACAAACTGGCGGTCAATTTCGGTGCTGAAATCCTCAAAATCGTACCCGGTCGCGTGTCAACAGAAGTTGATGCACGCCTCTCTTTTG  >  NC_000913/2578703‑2578972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A