Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 227 52.0 1764248 99.3% 1751898 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,769,457 G→A A265T (GCA→ACA)  yfjQ → CP4‑57 prophage; protein YfjQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,769,4570GA100.0% 181.4 / NA 51A265T (GCA→ACA) yfjQCP4‑57 prophage; protein YfjQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/26);  total (25/26)

CTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGT  >  NC_000913/2769324‑2769585
                                                                                                                                     |                                                                                                                                
cTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGaaa                                                                                                                            <  1:190015/140‑1 (MQ=255)
    cAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACt                                                                                                                        >  1:425538/1‑140 (MQ=255)
         gATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCa                                                                                                                   <  1:633094/140‑1 (MQ=255)
         gATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCa                                                                                                                   >  1:593688/1‑140 (MQ=255)
          aTTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCAc                                                                                                                  >  2:818825/1‑140 (MQ=255)
          aTTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCAc                                                                                                                  >  1:290808/1‑140 (MQ=255)
          aTTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCAc                                                                                                                  >  1:540519/1‑140 (MQ=255)
           ttCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACg                                                                                                                 <  2:255969/140‑1 (MQ=255)
                      cTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTa                                                                                                      <  1:449841/140‑1 (MQ=255)
                        gATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGa                                                                                                    >  2:529464/1‑140 (MQ=255)
                         aTTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGAc                                                                                                   >  1:581787/1‑140 (MQ=255)
                          ttAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACg                                                                                                  <  1:818825/140‑1 (MQ=255)
                                   gACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGtt                                                                                         >  1:868277/1‑140 (MQ=255)
                                      tCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGcc                                                                                      <  2:202557/140‑1 (MQ=255)
                                      tCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGcc                                                                                      <  1:788024/140‑1 (MQ=255)
                                      tCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGcc                                                                                      <  2:540519/140‑1 (MQ=255)
                                        aGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCAc                                                                                    <  1:605574/140‑1 (MQ=255)
                                        aGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCAc                                                                                    <  1:745361/140‑1 (MQ=255)
                                         gTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACg                                                                                   >  1:702031/1‑140 (MQ=255)
                                                  aTGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCAc                                                                                    <  1:873461/130‑1 (MQ=255)
                                                  aTGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCAc                                                                                    >  2:873461/1‑130 (MQ=255)
                                                  aTGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAata                                                                          >  1:836025/1‑140 (MQ=255)
                                                  aTGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAata                                                                          >  1:809104/1‑140 (MQ=255)
                                                        aaGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGac                                                                    <  1:709310/140‑1 (MQ=255)
                                                         aGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGaca                                                                   >  1:212233/1‑140 (MQ=255)
                                                          ggCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGacac                                                                  <  2:425538/140‑1 (MQ=255)
                                                              gACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACaccacc                                                              <  2:290808/140‑1 (MQ=255)
                                                               aCGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGa                                                                     >  1:714241/1‑132 (MQ=255)
                                                               aCGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGa                                                                     <  2:714241/132‑1 (MQ=255)
                                                               aCGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGa                                                                     <  1:576397/132‑1 (MQ=255)
                                                               aCGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGa                                                                     >  2:576397/1‑132 (MQ=255)
                                                               aCGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCt                                                             >  2:339204/1‑140 (MQ=255)
                                                               aCGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCt                                                             >  1:590544/1‑140 (MQ=255)
                                                                    cTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCg                                                        <  2:327207/140‑1 (MQ=255)
                                                                    cTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCg                                                        <  2:702031/140‑1 (MQ=255)
                                                                       aTACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCAt                                                     <  1:724156/140‑1 (MQ=255)
                                                                           tCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCt                                                 <  2:593688/140‑1 (MQ=255)
                                                                             gtgccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAt                                               >  1:316060/1‑140 (MQ=255)
                                                                               gccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAtgt                                             >  1:855019/1‑140 (MQ=255)
                                                                               gccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACGGCTCACGCAACTGCAGTAGACGCTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAtgt                                             >  2:723689/1‑140 (MQ=255)
                                                                                   tgcgtgGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTc                                         >  2:259515/1‑140 (MQ=255)
                                                                                       tgGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCgtgt                                     <  1:343332/140‑1 (MQ=255)
                                                                                                      tgtgAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCgg                      >  2:847761/1‑140 (MQ=255)
                                                                                                      tgtgAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCgg                      >  1:247288/1‑140 (MQ=255)
                                                                                                         gAAGCTTAACCGTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTta                   <  2:316060/140‑1 (MQ=255)
                                                                                                                    gTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAc        <  1:339204/140‑1 (MQ=255)
                                                                                                                    gTGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAc        <  1:259515/140‑1 (MQ=255)
                                                                                                                     tGCGCTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAcc       >  1:65304/1‑140 (MQ=255)
                                                                                                                       cgcTGTGGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAcccc     >  2:113452/1‑140 (MQ=255)
                                                                                                                          tgtgGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGt  <  1:428933/140‑1 (MQ=255)
                                                                                                                          tgtgGGTGATGACAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGt  <  1:595767/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                
CTATCAGCGGATTCAAGAAAACCTGATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGT  >  NC_000913/2769324‑2769585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A