Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 227 52.0 1764248 99.3% 1751898 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,791,898 T→C V209A (GTC→GCC)  gabD → NADP(+)‑dependent succinate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,791,8980TC100.0% 118.1 / NA 35V209A (GTC→GCC) gabDNADP(+)‑dependent succinate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/21);  total (14/21)

GGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGTCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGC  >  NC_000913/2791785‑2792032
                                                                                                                 |                                                                                                                                      
ggCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGTGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTa                                                                                                              >  1:623687/1‑140 (MQ=255)
     cagGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGaa                                                                                                         <  2:298381/140‑1 (MQ=255)
           gCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGAcc                                                                                                   <  1:105326/140‑1 (MQ=255)
                  ggTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAAcc                                                                                            <  2:47726/140‑1 (MQ=255)
                    tGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCg                                                                                          >  1:507901/1‑140 (MQ=255)
                           gCCCGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGGgc                                                                                   >  1:167437/1‑140 (MQ=255)
                              cGCCAGTCAGACGGCGTTTGCTGCGGTGGCGCTGGCGGAGCGGGCGGGCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCGCCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTATCCCGCTGGTGCGCa                                                                                <  2:498384/140‑1 (MQ=255)
                              cGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCa                                                                                >  2:245335/1‑140 (MQ=255)
                              cGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCa                                                                                <  2:528973/140‑1 (MQ=255)
                              cGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCa                                                                                >  1:819989/1‑140 (MQ=255)
                              cGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCa                                                                                >  1:376278/1‑140 (MQ=255)
                                ccAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCaaa                                                                              <  1:542485/140‑1 (MQ=255)
                                ccAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCaaa                                                                              >  2:267403/1‑140 (MQ=255)
                                 cAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAAc                                                                             <  2:235408/140‑1 (MQ=255)
                                   gTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTg                                                                           <  1:201724/140‑1 (MQ=255)
                                        aCGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGtt                                                                      >  1:108686/1‑140 (MQ=255)
                                              ttCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGGGCGGCCGGGAAGGGACCGACCAGTAAACCGCCGGTGCGCCAACTCTCGGTTAACgg                                                                >  2:201724/1‑140 (MQ=255)
                                               tctcTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGt                                                               <  1:821257/140‑1 (MQ=255)
                                               tctcTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGt                                                               <  1:143949/140‑1 (MQ=255)
                                                           cgctggcgGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAAtt                                                   >  2:264949/1‑140 (MQ=255)
                                                                 cgGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGgccgc                                             <  1:267403/140‑1 (MQ=255)
                                                                   gAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCa                                           <  1:245335/140‑1 (MQ=255)
                                                                   gAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCa                                           <  2:755757/140‑1 (MQ=255)
                                                                     gCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGgccg                                              <  1:412571/135‑1 (MQ=255)
                                                                     gCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGgccg                                              >  2:412571/1‑135 (MQ=255)
                                                                     gCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGt                                         <  2:623687/140‑1 (MQ=255)
                                                                        ggCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTaa                                      <  1:471932/140‑1 (MQ=255)
                                                                             tCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGaa                                 <  1:833779/140‑1 (MQ=255)
                                                                                  gcgGGCGTTCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTg                            <  1:264949/140‑1 (MQ=255)
                                                                                         ttCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGaa                                 >  2:45520/1‑128 (MQ=255)
                                                                                         ttCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGaa                                 <  1:45520/128‑1 (MQ=255)
                                                                                         ttCCGGCTGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGaaa                     <  2:108686/140‑1 (MQ=255)
                                                                                                tGGGGTATTTAACGTGGCCACCGGTTCGGCGGGCGCCGGCGGTAACGAACTGACCAGTAAACCGCTGGTGCGCAAACTGGCGGTTACCGGGTCGACCGAAATTGGCCGGCAGTTAATTGAAAAATGGGCGAAAGACATCa              >  2:272308/1‑140 (MQ=255)
                                                                                                       tttAACGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAgt       <  2:507901/140‑1 (MQ=255)
                                                                                                            cGTGGCCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGc  >  2:755763/1‑140 (MQ=255)
                                                                                                                 |                                                                                                                                      
GGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGGCGTTCCGGCTGGGGTATTTAACGTGGTCACCGGTTCGGCGGGCGCGGTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGC  >  NC_000913/2791785‑2792032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A