Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,832,355	G→A	intergenic (‑66/‑121)	norR ← / → norV	DNA‑binding transcriptional dual regulator NorR/anaerobic nitric oxide reductase flavorubredoxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,832,355	0	G	A	100.0%	112.5 / NA	32	intergenic (‑66/‑121)	norR/norV	DNA‑binding transcriptional dual regulator NorR/anaerobic nitric oxide reductase flavorubredoxin
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/18); total (14/18)

CGATCCTGGTGACCAATCCCACGCTGCAATTCGATGGCGATATTCGCCAGCACATCAACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGT  >  NC_000913/2832219‑2832486

cGATCCTGGTGACCAATCCCACGCTGCAATTCGATGGCGATATTCGCCAGCACATCAACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAact                                                                                                                                  <  2:753161/140‑1 (MQ=255)

        gTGACCAATCCCACGCTGCAATTCGATGGCGATATTCGCCAGCACATCAACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAACTACTCATTa                                                                                                                          <  1:851652/140‑1 (MQ=255)

         tGACCAATCCCACGCTGCAATTCGATGGCGATATTCGCCAGCACATCAACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAACTACTCATTaa                                                                                                                         <  2:605464/140‑1 (MQ=255)

               aTCCCACGCTGCAATTCGATGGCGATATTCGCCAGCACATCAACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCa                                                                                                                   >  2:125279/1‑140 (MQ=255)

                tCCCACGCTGCAATTCGATGGCGATATTCGCCAGCACATCAACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAact                                                                                                                                  <  1:763301/124‑1 (MQ=255)

                tCCCACGCTGCAATTCGATGGCGATATTCGCCAGCACATCAACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAact                                                                                                                                  >  2:763301/1‑124 (MQ=255)

                  ccACGCTGCAATTCGATGGCGATATTCGCCAGCACATCAACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCa                                                                                                                   >  2:724595/1‑137 (MQ=255)

                  ccACGCTGCAATTCGATGGCGATATTCGCCAGCACATCAACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCa                                                                                                                   <  1:724595/137‑1 (MQ=255)

                   cACGCTGCAATTCGATGGCGATATTCGCCAGCACATCAACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCATAAt                                                                                                               <  2:751661/140‑1 (MQ=255)

                      gCTGCAATTCGATGGCGATATTCGCCAGCACATCAACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCATAAtttt                                                                                                            <  2:591868/140‑1 (MQ=255)

                      gCTGCAATTCGATGGCGATATTCGCCAGCACATCAACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCATAAtttt                                                                                                            >  1:538050/1‑140 (MQ=255)

                            aTTCGATGGCGATATTCGCCAGCACATCAACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCATAATTttatttat                                                                                                      <  1:824033/140‑1 (MQ=255)

                                  tGGCGATATTCGCCAGCACATCAACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTa                                                                                                >  1:70915/1‑140 (MQ=255)

                                    gCGATATTCGCCAGCACATCAACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTaaa                                                                                              >  1:228617/1‑140 (MQ=255)

                                           tCGCCAGCACATCAACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATc                                                                                       <  1:8777/140‑1 (MQ=255)

                                                                             cTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCa                                                                   >  2:153101/1‑126 (MQ=255)

                                                                             cTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCa                                                                   <  1:153101/126‑1 (MQ=255)

                                                                                            aCTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGa                                      >  2:173537/1‑140 (MQ=255)

                                                                                                taGATATTGTCATATCGACCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAacac                                  <  2:858631/140‑1 (MQ=255)

                                                                                                          cATATCGACCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATaaa                        >  1:801950/1‑140 (MQ=255)

                                                                                                           atatCGACCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAAt                       >  2:787388/1‑140 (MQ=255)

                                                                                                               cGACCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAg                   <  1:787388/140‑1 (MQ=255)

                                                                                                                 aCCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAgg                  >  1:827942/1‑139 (MQ=255)

                                                                                                                 aCCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAgg                  <  2:827942/139‑1 (MQ=255)

                                                                                                                 aCCATTTGATTGATAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGt                 >  1:17823/1‑140 (MQ=255)

                                                                                                                    aTTTGATTGATAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGc              <  2:228617/140‑1 (MQ=255)

                                                                                                                     tttGATTGATAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCt             <  2:538050/140‑1 (MQ=255)

                                                                                                                     tttGATTGATAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCt             <  2:17823/140‑1 (MQ=255)

                                                                                                                     tttGATTGATAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCt             <  2:801950/140‑1 (MQ=255)

                                                                                                                       tgattgatAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTAt           >  2:55658/1‑140 (MQ=255)

                                                                                                                            gatAGTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAAAACGCTGAATAAATTGAGGTTGCTATGACTa      <  2:421365/140‑1 (MQ=255)

                                                                                                                                gTCATTTTAACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATtgt  >  2:170472/1‑140 (MQ=255)

CGATCCTGGTGACCAATCCCACGCTGCAATTCGATGGCGATATTCGCCAGCACATCAACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGT  >  NC_000913/2832219‑2832486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A