Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 227 52.0 1764248 99.3% 1751898 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,074,585 T→C L35L (TTA→TTG)  yggC ← P‑loop NTPase domain‑containing protein YggC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,074,5850TC100.0% 200.6 / NA 56L35L (TTA→TTG) yggCP‑loop NTPase domain‑containing protein YggC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/32);  total (24/32)

AGCGTCTGAATAGCGGGCAGTTCCGGGTCTTGTTGCGCGAGATATTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACT  >  NC_000913/3074454‑3074719
                                                                                                                                   |                                                                                                                                      
aGCGTCTGAATAGCGGGCAGTTCCGGGTCTTGTTGCGCGAGATATTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGgccgc                                                                                                                                >  1:375663/1‑140 (MQ=255)
 gCGTCTGAATAGCGGGCAGTTCCGGGTCTTGTTGCGCGAGATATTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGgccgcc                                                                                                                               <  1:100489/140‑1 (MQ=255)
    tCTGAATAGCGGGCAGTTCCGGGTCTTGTTGCGCGAGATATTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCGCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAAc                                                                                                                            <  1:772028/140‑1 (MQ=255)
    tCTGAATAGCGGGCAGTTCCGGGTCTTGTTGCGCGAGATATTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAAc                                                                                                                            >  1:865316/1‑140 (MQ=255)
    tCTGAATAGCGGGCAGTTCCGGGTCTTGTTGCGCGAGATATTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAAc                                                                                                                            <  2:624341/140‑1 (MQ=255)
     cTGAATAGCGGGCAGTTCCGGGTCTTGTTGCGCGAGATATTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACa                                                                                                                           >  1:229785/1‑140 (MQ=255)
     cTGAATAGCGGGCAGTTCCGGGTCTTGTTGCGCGAGATATTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACa                                                                                                                           <  2:172179/140‑1 (MQ=255)
             cGGGCAGTTCCGGGTCTTGTTGCGCGAGATATTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTgcagc                                                                                                                   >  2:538408/1‑140 (MQ=255)
             cGGGCAGTTCCGGGTCTTGTTGCGCGAGATATTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCAGATTTACCGTCTGCAAGGCCGCCAACATGTgcagc                                                                                                                   >  2:648602/1‑140 (MQ=255)
              gggCAGTTCCGGGTCTTGTTGCGCGAGATATTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTCCGCCGCTGCCGATTTTCCGCCCTCAAGCTCGCCAACATGTgcacca                                                                                                                  <  2:593642/140‑1 (MQ=255)
               ggCAGTTCCGGGTCTTGTTGCGCGAGATATTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCaa                                                                                                                 >  2:856945/1‑140 (MQ=255)
               ggCAGTTCCGGGTCTTGTTGCGCGAGATATTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCaa                                                                                                                 >  1:288567/1‑140 (MQ=255)
                gCAGTTCCGGGTCTTGTTGCGCGAGATATTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAAc                                                                                                                <  2:288567/140‑1 (MQ=255)
                                     cgAGATATTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAAtc                                                                                           >  1:579787/1‑140 (MQ=255)
                                      gagaTATTCCCAGAAGGAGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAAtct                                                                                          <  1:72753/140‑1 (MQ=255)
                                         atatTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCtt                                                                                       <  2:375663/140‑1 (MQ=255)
                                         atatTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCtt                                                                                       <  2:229785/140‑1 (MQ=255)
                                         atatTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCtt                                                                                       <  1:349596/140‑1 (MQ=255)
                                           atTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCg                                                                                     <  1:836729/140‑1 (MQ=255)
                                               ccAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCt                                                                                 <  2:466345/140‑1 (MQ=255)
                                                 aGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCtt                                                                                       <  1:532830/132‑1 (MQ=255)
                                                 aGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCtt                                                                                       >  2:532830/1‑132 (MQ=255)
                                                   aaGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCtt                                                                                       >  2:263478/1‑130 (MQ=255)
                                                   aaGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCtt                                                                                       <  1:263478/130‑1 (MQ=255)
                                                   aaGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTa                                                                             <  1:365811/140‑1 (MQ=255)
                                                     ggtggtTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACt                                                                           <  2:95234/140‑1 (MQ=255)
                                                            aGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTg                                                                    <  1:538408/140‑1 (MQ=255)
                                                             gggTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGt                                                                   >  2:228015/1‑140 (MQ=255)
                                                               gTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCCGTAt                                                                 >  1:434079/1‑140 (MQ=255)
                                                                 tGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCt                                                               <  2:251426/140‑1 (MQ=255)
                                                                 tGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCt                                                               >  2:870862/1‑140 (MQ=255)
                                                                     ttACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAg                                                           <  1:856945/140‑1 (MQ=255)
                                                                      tACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGc                                                          <  2:579787/140‑1 (MQ=255)
                                                                        cccGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGccc                                                        >  2:443711/1‑140 (MQ=255)
                                                                           gTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCAtt                                                     <  1:228015/140‑1 (MQ=255)
                                                                             ccccGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTg                                                                    >  1:748751/1‑123 (MQ=255)
                                                                             ccccGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTg                                                                    <  2:748751/123‑1 (MQ=255)
                                                                             ccccGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCa                                                   >  1:675153/1‑140 (MQ=255)
                                                                             ccccGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCa                                                   >  1:696622/1‑140 (MQ=255)
                                                                                        cgcAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCa                                        >  2:165420/1‑140 (MQ=255)
                                                                                               aaaaaCCACCGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCa                                 <  1:165420/140‑1 (MQ=255)
                                                                                                       ccGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTa                         >  2:872057/1‑140 (MQ=255)
                                                                                                        cGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTaa                        <  2:434079/140‑1 (MQ=255)
                                                                                                        cGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTaa                        <  2:18892/140‑1 (MQ=255)
                                                                                                        cGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTaa                        <  1:870862/140‑1 (MQ=255)
                                                                                                        cGTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTaa                        <  2:696622/140‑1 (MQ=255)
                                                                                                        cGTACGCCGCTGCAGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTaa                        <  1:648602/140‑1 (MQ=255)
                                                                                                         gTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTaa                        >  1:820373/1‑139 (MQ=255)
                                                                                                         gTACGCCGCTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTaa                        <  2:820373/139‑1 (MQ=255)
                                                                                                              ccgcTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCtt                  <  2:675153/140‑1 (MQ=255)
                                                                                                               cgcTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCttt                 >  1:857965/1‑140 (MQ=255)
                                                                                                                 cTGCGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTaa               >  1:57400/1‑140 (MQ=255)
                                                                                                                    cGGATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAggt            >  2:518761/1‑140 (MQ=255)
                                                                                                                      gATTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAggtgg          <  1:872057/140‑1 (MQ=255)
                                                                                                                       aTTTACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAggtggt         <  1:518761/140‑1 (MQ=255)
                                                                                                                          tACCGTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGt      <  2:395405/140‑1 (MQ=255)
                                                                                                                              gTCTGCAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACt  >  2:126034/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                      
AGCGTCTGAATAGCGGGCAGTTCCGGGTCTTGTTGCGCGAGATATTCCCAGAAGGTGGTTAGGGTTGATTTACCCGTCCCCGGCGGAGCGCAAAGAAAAACCACCGTACGCCGCTGCGGATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACT  >  NC_000913/3074454‑3074719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A