Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 227 52.0 1764248 99.3% 1751898 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,696,540 T→C V28A (GTC→GCC)  bcsE → c‑di‑GMP‑binding protein BcsE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,696,5400TC100.0% 258.5 / NA 73V28A (GTC→GCC) bcsEc‑di‑GMP‑binding protein BcsE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/33);  total (40/33)

TAGGTTTATTTCTTAGCTTTCGCTAGTAAACTGATAATCAGACAAAATGGTGACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGA  >  NC_000913/3696404‑3696675
                                                                                                                                        |                                                                                                                                       
tAGGTTTATTTCTTAGCTTTCGCTAGTAAACTGATAATCAGACAAAATGGTGACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCtg                                                                                                                                      <  2:785006/140‑1 (MQ=255)
 aGGTTTATTTCTTAGCTTTCGCTAGTAAACTGATAATCCGACAAAATGGTGACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCtgg                                                                                                                                     <  2:740729/140‑1 (MQ=255)
              aGCTTTCGCTAGTAAACTGATAATCAGACAAAATGGTGACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCg                                                                                                                        >  2:131717/1‑140 (MQ=255)
               gCTTTCGCTAGTAAACTGATAATCAGACAAAATGGTGACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGa                                                                                                                       <  1:874041/140‑1 (MQ=255)
                   tCGCTAGTAAACTGATAATCAGACAAAATGGTGACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGc                                                                                                                   >  1:672044/1‑140 (MQ=255)
                   tCGCTAGTAAACTGATAATCAGACAAAATGGTGACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGc                                                                                                                   >  1:726394/1‑140 (MQ=255)
                          tAAACTGATAATCAGACAAAATGGTGACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAg                                                                                                            <  2:291163/140‑1 (MQ=255)
                           aaaCTGATAATCAGACAAAATGGTGACATGAGGGACATTGTGGACCCTGTATTCTCTATCTGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGa                                                                                                           <  2:380335/140‑1 (MQ=255)
                            aaCTGATAATCAGACAAAATGGTGACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGAt                                                                                                          >  1:859936/1‑140 (MQ=255)
                              cTGATAATCAGAC‑AAATGGTGACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCt                                                                                                       <  2:442609/140‑1 (MQ=255)
                                 aTAATCAGACAAAATGGTGACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTAt                                                                                                     >  1:557370/1‑140 (MQ=255)
                                        gACAAAATGGTGACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATCAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTg                                                                                              <  2:616042/140‑1 (MQ=255)
                                         aCAAAATGGTGACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTgg                                                                                             >  2:796170/1‑140 (MQ=255)
                                           aaaaTGGTGACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCg                                                                                           >  2:210614/1‑140 (MQ=255)
                                             aaTGGTGACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGaa                                                                                         >  2:591478/1‑140 (MQ=255)
                                                   gACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAAc                                                                                   >  1:3584/1‑140 (MQ=255)
                                                   gACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAAc                                                                                   >  1:342248/1‑140 (MQ=255)
                                                   gACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAAc                                                                                   >  1:318014/1‑140 (MQ=255)
                                                   gACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAAc                                                                                   >  2:361921/1‑140 (MQ=255)
                                                   gACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAAc                                                                                   <  2:859936/140‑1 (MQ=255)
                                                     cATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACaa                                                                                 >  1:290009/1‑140 (MQ=255)
                                                             aCATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATcc                                                                         <  1:254020/140‑1 (MQ=255)
                                                               aTTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCa                                                                       >  2:678400/1‑140 (MQ=255)
                                                                ttGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAg                                                                      >  1:256802/1‑140 (MQ=255)
                                                                          tGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAgg                                                                     >  1:446174/1‑131 (MQ=255)
                                                                          tGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAgg                                                                     <  2:446174/131‑1 (MQ=255)
                                                                          tGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCg                                                            >  1:191351/1‑140 (MQ=255)
                                                                          tGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCg                                                            >  2:80602/1‑140 (MQ=255)
                                                                          tGTATTCTCTATCGGTATATCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATcc                                                                         >  1:593893/1‑127 (MQ=255)
                                                                          tGTATTCTCTATCGGTATATCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATcc                                                                         <  2:593893/127‑1 (MQ=255)
                                                                           gTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGc                                                           >  2:249829/1‑140 (MQ=255)
                                                                             aTTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGcac                                                         >  2:92724/1‑140 (MQ=255)
                                                                              ttCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGcaca                                                        <  1:92724/140‑1 (MQ=255)
                                                                                   tATCGGTATCTCATCATTATGGGATGAGCTGCGATATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTcg                                                   <  1:231396/140‑1 (MQ=255)
                                                                                   tATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTcg                                                   <  1:591478/140‑1 (MQ=255)
                                                                                   tATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTcg                                                   <  1:249829/140‑1 (MQ=255)
                                                                                   tATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTcg                                                   <  2:726394/140‑1 (MQ=255)
                                                                                   tATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTcg                                                   <  2:712217/140‑1 (MQ=255)
                                                                                    aTCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTcgc                                                  >  2:631873/1‑140 (MQ=255)
                                                                                     tCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTcgcg                                                 <  2:672044/140‑1 (MQ=255)
                                                                                      cGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCgg                                                >  1:224099/1‑140 (MQ=255)
                                                                                      cGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCgg                                                >  1:774859/1‑140 (MQ=255)
                                                                                        gTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTcgcg                                                 >  2:664814/1‑137 (MQ=255)
                                                                                        gTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTcgcg                                                 <  1:664814/137‑1 (MQ=255)
                                                                                        gTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTc                                              >  1:699808/1‑140 (MQ=255)
                                                                                              catcatTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGc                                        <  1:210614/140‑1 (MQ=255)
                                                                                              catcatTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGc                                        <  1:796170/140‑1 (MQ=255)
                                                                                               atcatTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGc                                        <  1:386270/139‑1 (MQ=255)
                                                                                               atcatTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGc                                        >  2:386270/1‑139 (MQ=255)
                                                                                               atcatTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCa                                       <  2:342248/140‑1 (MQ=255)
                                                                                               atcatTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCa                                       >  1:356044/1‑140 (MQ=255)
                                                                                                   ttATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGa                                   >  1:70334/1‑140 (MQ=255)
                                                                                                   ttATGGAATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATg                                     <  1:108064/138‑1 (MQ=255)
                                                                                                   ttATGGAATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATg                                     >  2:108064/1‑138 (MQ=255)
                                                                                                         gATGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGa                             >  1:641340/1‑140 (MQ=255)
                                                                                                          aTGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGAt                            <  2:699808/140‑1 (MQ=255)
                                                                                                          aTGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGAt                            <  1:678400/140‑1 (MQ=255)
                                                                                                           tGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATc                           >  1:801436/1‑140 (MQ=255)
                                                                                                           tGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATc                           >  1:480838/1‑140 (MQ=255)
                                                                                                           tGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATc                           >  2:650849/1‑140 (MQ=255)
                                                                                                           tGAGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATc                           >  2:391904/1‑140 (MQ=255)
                                                                                                             aGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATCCg                         >  1:879265/1‑140 (MQ=255)
                                                                                                             aGCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATCCg                         >  2:186924/1‑140 (MQ=255)
                                                                                                              gCTGCGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATCCgg                        >  2:205708/1‑140 (MQ=255)
                                                                                                                  cGACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATCCGGCGaa                    <  2:480838/140‑1 (MQ=255)
                                                                                                                   gACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATCCGGCGaaa                   >  2:761794/1‑140 (MQ=255)
                                                                                                                   gACATATGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATCCGGCGaaa                   <  2:318014/140‑1 (MQ=255)
                                                                                                                       tatGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCt               <  2:774859/140‑1 (MQ=255)
                                                                                                                         tGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCttt             <  1:631873/140‑1 (MQ=255)
                                                                                                                         tGCCAGCAGGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCttt             >  1:389398/1‑140 (MQ=255)
                                                                                                                              gcagGCGGCGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAAtt        <  2:557370/140‑1 (MQ=255)
                                                                                                                                 ggcggcGCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAga     <  2:224099/140‑1 (MQ=255)
                                                                                                                                    ggcgCCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAgatga  <  1:650849/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
TAGGTTTATTTCTTAGCTTTCGCTAGTAAACTGATAATCAGACAAAATGGTGACATGAGGGACATTGTGGACCCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGA  >  NC_000913/3696404‑3696675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A