Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,818,188	T→C	H143H (CAT→CAC)	dinD →	DNA damage‑inducible protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,818,188	0	T	C	100.0%	128.3 / NA	37	H143H (CAT→CAC)	dinD	DNA damage‑inducible protein D
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/23); total (14/23)

CGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGACCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACATAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGTATGGTGGATTAGATCAGAAAGCTATTCATCAGCGGAAGGGGCTG  >  NC_000913/3818058‑3818323

cGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGACCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAacaaacaa                                                                                                                                <  1:246609/140‑1 (MQ=255)

                              cTATCCAGACCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAACAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTa                                                                                                  >  2:325253/1‑140 (MQ=255)

                              cTATCCAGACCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAg                                                                                                          <  2:371804/132‑1 (MQ=255)

                              cTATCCAGACCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAg                                                                                                          >  1:371804/1‑132 (MQ=255)

                                aTCCAGACCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGc                                                                                                <  1:325253/140‑1 (MQ=255)

                                aTCCAGACCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGc                                                                                                >  1:717273/1‑140 (MQ=255)

                                  ccAGACCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTa                                                                                                  >  2:387586/1‑136 (MQ=255)

                                  ccAGACCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTa                                                                                                  <  1:387586/136‑1 (MQ=255)

                                  ccAGACCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGa                                                                                              <  2:567832/140‑1 (MQ=255)

                                  ccAGACCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGa                                                                                              <  1:800022/140‑1 (MQ=255)

                                  ccAGACCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGa                                                                                              <  2:67618/140‑1 (MQ=255)

                                  ccAGACCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGa                                                                                              <  2:717273/140‑1 (MQ=255)

                                      aCCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGc                                                                                          >  2:42779/1‑140 (MQ=255)

                                      aCCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGc                                                                                          >  1:6721/1‑140 (MQ=255)

                                        ccGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTa                                                                                        >  1:517275/1‑140 (MQ=255)

                                           aCGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTg                                                                                     >  2:474563/1‑140 (MQ=255)

                                                       tGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCatat                                                                         >  2:831320/1‑140 (MQ=255)

                                                                    gCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATgg                                                            <  2:6721/140‑1 (MQ=255)

                                                                           aGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAg                                                     <  2:126699/140‑1 (MQ=255)

                                                                            gCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAgg                                                    <  1:412710/140‑1 (MQ=255)

                                                                            gCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTCGGGTAGCGACAACTACTGATTTCGCCATATTTCAGAATCATGGTTACCAgg                                                    <  1:148397/140‑1 (MQ=255)

                                                                              aaCTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAgggg                                                  <  1:574027/140‑1 (MQ=255)

                                                                                  tCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGt                                              >  2:288885/1‑140 (MQ=255)

                                                                                  tCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGt                                              >  1:242826/1‑140 (MQ=255)

                                                                                   cGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGTa                                             <  1:831320/140‑1 (MQ=255)

                                                                                     tGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGTAtg                                           <  1:474563/140‑1 (MQ=255)

                                                                                      gAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTGCCAGGGGCTGTAtgg                                          <  2:556463/140‑1 (MQ=255)

                                                                                      gAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGTAtgg                                          <  2:517275/140‑1 (MQ=255)

                                                                                          aCGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGTATGGTGGa                                      >  2:710706/1‑140 (MQ=255)

                                                                                            gAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGTATGGTGGAtt                                    <  2:162685/140‑1 (MQ=255)

                                                                                                     cTGTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGTATGGTGGATTAGATCAGaa                           <  1:106868/140‑1 (MQ=255)

                                                                                                       gTTTCTGAGAAATGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGTATGGTGGATTAGATCAGAAAg                         <  2:662524/140‑1 (MQ=255)

                                                                                                                  aTGAGTTGAAAGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGTATGGTGGATTAGATCAGAAAGCTATTCATCAg              >  2:625225/1‑140 (MQ=255)

                                                                                                                          aaaGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGTATGGTGGATTAGATCAGAAAGCTATTCATCAGCGGAAggg      >  2:531551/1‑140 (MQ=255)

                                                                                                                           aaGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGTATGGTGGATTAGATCAGAAAGCTATTCATCAGCGGAAgggg     <  1:531551/140‑1 (MQ=255)

                                                                                                                           aaGAACACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGTATGGTGGATTAGATCAGAAAGCTATTCATCAGCGGAAgggg     <  1:288885/140‑1 (MQ=255)

                                                                                                                              aaCACAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGTATGGTGGATTAGATCAGAAAGCTATTCATCAGCGGAAGGGGCTg  <  1:822837/140‑1 (MQ=255)

CGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGACCCGACGGCAGGAGCTTGCTGACGATGAAGCATTTAAGCAACTTCGTGAAGACGAAAAGCGTCTGTTTCTGAGAAATGAGTTGAAAGAACATAACAAACAATTGGTTGAGGCAGCACAGCAAGCTGGGGTAGCGACAGCTACTGATTTCGCCATATTTCAGAATCATGGTTACCAGGGGCTGTATGGTGGATTAGATCAGAAAGCTATTCATCAGCGGAAGGGGCTG  >  NC_000913/3818058‑3818323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A