Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,924,470	A→G	G388G (GGT→GGC)	mnmG ←	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,924,470	0	A	G	100.0%	186.8 / NA	54	G388G (GGT→GGC)	mnmG	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/23); total (31/23)

TCTGCGCGCGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGACCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTGCTCTCCAGCGTCGGTTTCAGGTCGCGAGGATCGAAGAAGTCATACTCAA  >  NC_000913/3924333‑3924606

tCTGCGCGCGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGcc                                                                                                                                        >  1:675808/1‑140 (MQ=255)

  tGCGCGCGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCgg                                                                                                                                      >  2:312473/1‑140 (MQ=255)

   gcgcgcGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGc                                                                                                                                     >  1:40015/1‑140 (MQ=255)

       gcgAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGc                                                                                                                                 <  2:714924/140‑1 (MQ=255)

       gcgAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGc                                                                                                                                 >  2:627452/1‑140 (MQ=255)

       gcgAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGc                                                                                                                                 <  1:835498/140‑1 (MQ=255)

                aCATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTgc                                                                                                                        <  2:418555/140‑1 (MQ=255)

                     cgatacgGTTCTTTGGTTCCTAAAGTGCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGc                                                                                                                   >  1:752099/1‑140 (MQ=255)

                       atacgGTTCTTTGGTTCCTAAAGTGCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCgg                                                                                                                 >  1:451229/1‑140 (MQ=255)

                       atacgGTTCTTTGGTTCCTAAAGTGCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGCCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCgg                                                                                                                 >  2:93110/1‑140 (MQ=255)

                            gTTCTTTGGTTCCTAAAGTGCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGcttct                                                                                                            >  2:411216/1‑140 (MQ=255)

                            gTTCTTTGGTTCCTAAAGTGCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGcttct                                                                                                            <  2:573157/140‑1 (MQ=255)

                                       ccTAAAGTGCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGt                                                                                                 >  2:828609/1‑140 (MQ=255)

                                         tAAAGTGCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAg                                                                                               <  1:828609/140‑1 (MQ=255)

                                               gCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGt                                                                                         >  1:744017/1‑140 (MQ=255)

                                                cacaGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGtt                                                                                        >  2:391454/1‑140 (MQ=255)

                                                   aGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAAt                                                                                     <  1:411216/140‑1 (MQ=255)

                                                   aGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAAt                                                                                     <  1:312473/140‑1 (MQ=255)

                                                        atcaACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGAc                                                                                >  2:112388/1‑140 (MQ=255)

                                                        atcaACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGAc                                                                                <  1:112388/140‑1 (MQ=255)

                                                        atcaACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGAc                                                                                >  1:171343/1‑140 (MQ=255)

                                                             cTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCa                                                                           <  2:744017/140‑1 (MQ=255)

                                                              tAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCaa                                                                          <  2:451229/140‑1 (MQ=255)

                                                               aGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCaaa                                                                         >  1:608184/1‑140 (MQ=255)

                                                               aGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCaaa                                                                         >  1:331854/1‑140 (MQ=255)

                                                               aGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCaaa                                                                         >  2:503555/1‑140 (MQ=255)

                                                                gTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAg                                                                        >  2:107472/1‑140 (MQ=255)

                                                                   cGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAAc                                                                     >  2:225390/1‑140 (MQ=255)

                                                                   cGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTCCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAAc                                                                     >  2:680207/1‑140 (MQ=255)

                                                                       gagaTACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGcc                                                                 >  2:458414/1‑140 (MQ=255)

                                                                        agaTACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGccc                                                                <  2:608184/140‑1 (MQ=255)

                                                                        agaTACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGccc                                                                >  2:475039/1‑140 (MQ=255)

                                                                         gaTACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGcccc                                                               <  1:225390/140‑1 (MQ=255)

                                                                          aTACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCt                                                              >  1:420921/1‑140 (MQ=255)

                                                                             cGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGa                                                           >  2:663350/1‑140 (MQ=255)

                                                                                cTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATaa                                                        >  2:188019/1‑140 (MQ=255)

                                                                                  gagaACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAAc                                                      <  1:475039/140‑1 (MQ=255)

                                                                                    gaACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACtt                                                    >  1:481895/1‑140 (MQ=255)

                                                                                     aaCGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTg                                                   <  1:503555/140‑1 (MQ=255)

                                                                                      aCGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTGc                                                  <  1:107472/140‑1 (MQ=255)

                                                                                             ggAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTGCTCTCCAg                                           >  2:112966/1‑140 (MQ=255)

                                                                                               aGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTGCTCTCCAGCg                                         >  2:611246/1‑140 (MQ=255)

                                                                                                gCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTGCTCTCCAGCGt                                        <  2:811858/140‑1 (MQ=255)

                                                                                                      ccTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTGCTCTCCAGCGTCGGttt                                  >  1:441277/1‑140 (MQ=255)

                                                                                                          cttTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTGCTCTCCAGCGTCGGTTTCAgg                              >  1:731158/1‑140 (MQ=255)

                                                                                                          cttTGTCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTGCTCTCCAGCGTCGGTTTCAgg                              >  2:782700/1‑140 (MQ=255)

                                                                                                               tCAGCAGACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTGCTCTCCAGCGTCGGTTTCAGGTcgcg                         <  2:331854/140‑1 (MQ=255)

                                                                                                                cagcagACAGACGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTGCTCTCCAGCGTCGGTTTCAGGTCGCGa                        >  2:564345/1‑140 (MQ=255)

                                                                                                                         gacGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTGCTCTCCAGCGTCGGTTTCAGGTCGCGAGGATCgaag               <  1:458414/140‑1 (MQ=255)

                                                                                                                         gacGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTGCTCTCCAGCGTCGGTTTCAGGTCGCGAGGATCgaag               <  1:782700/140‑1 (MQ=255)

                                                                                                                         gacGGGCAGCGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTGCTCTCCAGCGTCGGTTTCAGGTCGCGAGGATCgaag               <  1:135673/140‑1 (MQ=255)

                                                                                                                                  cGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTGCTCTCCAGCGTCGGTTTCAGGTCGCGAGGATCgaa                <  2:131572/130‑1 (MQ=255)

                                                                                                                                  cGTTAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTGCTCTCCAGCGTCGGTTTCAGGTCGCGAGGATCgaa                >  1:131572/1‑130 (MQ=255)

                                                                                                                                     tAAGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTGCTCTCCAGCGTCGGTTTCAGGTCGCGAGGATCGAAGAAGTCATACTCa   <  1:663350/140‑1 (MQ=255)

                                                                                                                                      aaGGCCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTGCTCTCCAGCGTCGGTTTCAGGTCGCGAGGATCGAAGAAGTCATACTCaa  <  1:611246/140‑1 (MQ=255)

TCTGCGCGCGAAGTAAACATACGATACGGTTCTTTGGTTCCTAAAGTGCACAGGTCATCAACTAGTACGCCGAGATACGCCTGAGAACGTGCCGGAGCCCAACCTTCTTTGTCAGCAGACAGACGGGCAGCGTTAAGACCGGCCAGCAAACCTTGCGCAGCGGCTTCTTCGTAACCGGTAGTGCCGTTAATCTGACCAGCAAAGAACAGCCCCTGGATAAACTTGCTCTCCAGCGTCGGTTTCAGGTCGCGAGGATCGAAGAAGTCATACTCAA  >  NC_000913/3924333‑3924606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A