Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F28 I1 R1
|
227 |
52.0 |
1764248 |
99.3% |
1751898 |
138.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,956,754 |
T→C |
V475A (GTA→GCA) |
ilvA → |
threonine deaminase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,956,754 | 0 | T | C | 100.0%
| 74.3
/ NA
| 22 | V475A (GTA→GCA) | ilvA | threonine deaminase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (11/11); total (11/11) |
GAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAA > NC_000913/3956627‑3956879
|
gAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGtt < 2:634352/140‑1 (MQ=255)
aCGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCg < 1:85940/140‑1 (MQ=255)
gCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGAc < 1:847909/140‑1 (MQ=255)
tCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCAt > 2:220491/1‑140 (MQ=255)
gAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCATGa > 2:829890/1‑140 (MQ=255)
tCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGa < 2:688314/140‑1 (MQ=255)
aCCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGaaa < 1:220491/140‑1 (MQ=255)
cGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAAcc > 1:764749/1‑140 (MQ=255)
gcgcgcTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCgg < 2:764749/140‑1 (MQ=255)
ttCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTggg < 2:799925/140‑1 (MQ=255)
acacGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGAt > 2:228730/1‑140 (MQ=255)
acTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAAcc > 2:496552/1‑140 (MQ=255)
ggAACATTTCTTTGTTCCACTGTCGCAGCGATGCCACCGAAGACCGGCGCGCACTGGCGCCGGCCGAACCACCCCCCCATGAACCGGGTTTAGAAA‑CCGGCTGAATGAGCTGGGCCACGCTGGCCACGACGAAACCAATa > 2:784998/1‑140 (MQ=255)
ggAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAAt > 2:179699/1‑140 (MQ=255)
ggAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAAt > 2:32558/1‑140 (MQ=255)
aaCATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATaa > 2:585558/1‑140 (MQ=255)
ttGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGtt < 1:585558/140‑1 (MQ=255)
ttGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGtt < 1:496552/140‑1 (MQ=255)
cTATCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCt > 1:580821/1‑140 (MQ=255)
tCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCtttt > 1:626101/1‑140 (MQ=255)
tCGCAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCtttt > 2:703255/1‑140 (MQ=255)
cAGCCATGGCACCGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTgg < 1:32558/140‑1 (MQ=255)
cGACTACGGGCGCGCACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGaa < 2:626101/140‑1 (MQ=255)
|
GAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAA > NC_000913/3956627‑3956879
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
