Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 227 52.0 1764248 99.3% 1751898 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,970,845 A→G D238G (GAC→GGC)  wecB → UDP‑N‑acetylglucosamine 2‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,970,8450AG100.0% 115.0 / NA 33D238G (GAC→GGC) wecBUDP‑N‑acetylglucosamine 2‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/22);  total (11/22)

GCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGCTGA  >  NC_000913/3970718‑3970970
                                                                                                                               |                                                                                                                             
gcAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGt                                                                                                                   <  1:705558/140‑1 (MQ=255)
    aTTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTAt                                                                                                               <  2:564971/140‑1 (MQ=255)
     ttACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATc                                                                                                              <  1:757678/140‑1 (MQ=255)
                     cccGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAAccc                                                                                              >  2:604810/1‑140 (MQ=255)
                        gATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGaa                                                                                           <  2:786198/140‑1 (MQ=255)
                                gatgatTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCagag                                                                                   <  2:4451/140‑1 (MQ=255)
                                  tgatTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGaa                                                                                 <  1:206766/140‑1 (MQ=255)
                                       cTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGt                                                                            <  1:186718/140‑1 (MQ=255)
                                       cTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGt                                                                            <  2:663937/140‑1 (MQ=255)
                                          gTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGt                                                                            <  2:216694/137‑1 (MQ=255)
                                          gTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGt                                                                            >  1:216694/1‑137 (MQ=255)
                                            gACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATc                                                                       <  1:536728/140‑1 (MQ=255)
                                            gACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATc                                                                       <  2:241796/140‑1 (MQ=255)
                                              ccGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGc                                                                     <  1:604810/140‑1 (MQ=255)
                                               cGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATc                                                                       >  1:156728/1‑137 (MQ=255)
                                               cGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATc                                                                       <  2:156728/137‑1 (MQ=255)
                                                       ggCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTgggg                                                            <  2:584302/140‑1 (MQ=255)
                                                        gCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGc                                                           >  1:194141/1‑140 (MQ=255)
                                                        gCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGc                                                           >  1:795864/1‑140 (MQ=255)
                                                                  ttCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGaaaaa                                                 >  1:837360/1‑140 (MQ=255)
                                                                    cGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATg                                               <  1:607539/140‑1 (MQ=255)
                                                                    cGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATg                                               <  2:795864/140‑1 (MQ=255)
                                                                           ggCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCt                                        >  2:435487/1‑140 (MQ=255)
                                                                              tttGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTgat                                     <  2:654509/140‑1 (MQ=255)
                                                                                tGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTgatcg                                   <  2:534973/140‑1 (MQ=255)
                                                                                         cTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGTATGTGAAAAATGTCATTCTGATCGATCCCCAgg                          <  1:435487/140‑1 (MQ=255)
                                                                                            ccACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTgat                                     >  2:192107/1‑126 (MQ=255)
                                                                                            ccACGCGCTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTgat                                     <  1:192107/126‑1 (MQ=255)
                                                                                                 cgcTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAgg                          <  1:847826/132‑1 (MQ=255)
                                                                                                 cgcTGGCAGACATCGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAgg                          >  2:847826/1‑132 (MQ=255)
                                                                                                              cGCCACCACGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGc     >  1:755604/1‑140 (MQ=255)
                                                                                                                ccaccaCGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGCtg   >  1:224130/1‑140 (MQ=255)
                                                                                                                 caccacGCACCAGGGCATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGCtga  <  1:474694/140‑1 (MQ=255)
                                                                                                                               |                                                                                                                             
GCAAATTACCCGTTTATCGACCCCGATAAAAAGATGATTCTGGTGACCGGTCACAGGCGTGAGAGTTTCGGTCGTGGCTTTGAAGAAATCTGCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGCTGA  >  NC_000913/3970718‑3970970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A