Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,104,922	C→T	A18A (GCG→GCA)	cpxA ←	sensory histidine kinase CpxA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,104,922	0	C	T	100.0%	132.6 / NA	38	A18A (GCG→GCA)	cpxA	sensory histidine kinase CpxA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/20); total (18/20)

TGGGCGGATCGTTCGCCAGCTCCGCTTCGACATGCTGCTCAATCATCAGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGCGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCACGCAAGGTTTTAAACCACGGGTGACCATCTTTACGATCCGGCAGTTTACGACG  >  NC_000913/4104789‑4105058

tGGGCGGATCGTTCGCCAGCTCCGCTTCGACATGCTGCTCAATCATCAGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACAccagtgccagc                                                                                                                                    >  1:416695/1‑140 (MQ=255)

 gggCGGATCGTTCGCCAGCTCCGCTTCGACATGCTGCTCAATCATCAGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACAccagtgccagcg                                                                                                                                   <  1:3524/140‑1 (MQ=255)

 gggCGGATCGTTCGCCAGCTCCGCTTCGACATGCTGCTCAATCATCAGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACAccagtgccagcg                                                                                                                                   <  2:710236/140‑1 (MQ=255)

  ggCGGATCGTTCGCCAGCTCCGCTTCGACATGCTGCTCAATCATCAGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGt                                                                                                                                  <  2:329691/140‑1 (MQ=255)

    cGGATCGTTCGCCAGCTCCGCTTCGACATGCTGCTCAATCATCAGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTc                                                                                                                                 >  1:427222/1‑139 (MQ=255)

    cGGATCGTTCGCCAGCTCCGCTTCGACATGCTGCTCAATCATCAGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTc                                                                                                                                 <  2:427222/139‑1 (MQ=255)

    cGGATCGTTCGCCAGCTCCGCTTCGACATGCTGCTCAATCATCAGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCa                                                                                                                                >  1:723991/1‑140 (MQ=255)

     ggATCGTTCGCCAGCTCCGCTTCGACATGCTGCTCAATCATCAGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAg                                                                                                                               <  2:416695/140‑1 (MQ=255)

      gATCGTTCGCCAGCTCCGCTTCGACATGCTGCTCAATCATCAGCCCCTGACGATGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGc                                                                                                                              <  1:825159/140‑1 (MQ=255)

        tCGTTCGCCAGCTCCGCTTCGACATGCTGCTCAATCATCAGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCa                                                                                                                            >  1:754845/1‑140 (MQ=255)

            tCGCCAGCTCCGCTTCGACATGCTGCTCAATCATCAGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCagaag                                                                                                                        >  2:686685/1‑140 (MQ=255)

                         ttCGACATGCTGCTCAATCATCAGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATgc                                                                                                           <  2:668114/140‑1 (MQ=255)

                            gACATGCTGCTCAATCATCAGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATgcgcg                                                                                                        <  2:359891/140‑1 (MQ=255)

                             aCATGCTGCTCAATCATCAGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATgcgcgc                                                                                                       <  2:468781/140‑1 (MQ=255)

                                tgctgcTCAATCATCAGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGt                                                                                                    <  2:10729/140‑1 (MQ=255)

                                   tgctCAATCATCAGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTaa                                                                                                 <  1:777921/140‑1 (MQ=255)

                                         atcatcAGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGcc                                                                                           <  1:56477/140‑1 (MQ=255)

                                          tcatcaGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCt                                                                                          >  1:714219/1‑140 (MQ=255)

                                                gCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGTGGTTAAGCTGCCTATCATg                                                                                    <  1:686685/140‑1 (MQ=255)

                                                 ccccTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGa                                                                                   >  2:289652/1‑140 (MQ=255)

                                                    cTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGc                                                                                >  1:733200/1‑140 (MQ=255)

                                                       aCGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGa                                                                             <  1:289652/140‑1 (MQ=255)

                                                           tGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAAcc                                                                         >  2:75099/1‑140 (MQ=255)

                                                                    tCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGAtgg                                                                >  1:878199/1‑138 (MQ=255)

                                                                              cGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCAc                                                      >  2:831809/1‑140 (MQ=255)

                                                                              cGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCAc                                                      >  1:623119/1‑140 (MQ=255)

                                                                              cGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCAc                                                      >  2:41458/1‑140 (MQ=255)

                                                                               ggTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCACg                                                     <  1:41458/140‑1 (MQ=255)

                                                                                 tCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCACGCa                                                   <  1:825575/140‑1 (MQ=255)

                                                                                       ggCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCACGCa                                                   <  2:47749/134‑1 (MQ=255)

                                                                                       ggCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCACGCa                                                   >  1:47749/1‑134 (MQ=255)

                                                                                       ggCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCACGCAAGGttt                                             <  2:623119/140‑1 (MQ=255)

                                                                                         cGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCACGCAAGGTTTTa                                           >  2:393880/1‑140 (MQ=255)

                                                                                           tGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCACGCAAGGTTTTaaa                                         <  1:831809/140‑1 (MQ=255)

                                                                                                   gCTTGGGTAACATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCACGCAAGGTTTTAAACCACGGGt                                 <  2:733200/140‑1 (MQ=255)

                                                                                                             cATCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCACGCAAGGTTTTAAACCACGGGTGACCATCttt                       >  1:754046/1‑140 (MQ=255)

                                                                                                              aTCAAAACCAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCACGCAAGGTTTGAAACCACGGGTGACCATCTTTa                      >  1:626305/1‑140 (MQ=255)

                                                                                                                      cAACATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCACGCAAGGTTTTAAACCACGGGTGACCATCTTTACGATCCgg              <  2:371885/140‑1 (MQ=255)

                                                                                                                        aCATCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCACGCAAGGTTTTAAACCACGGGTGACCATCTTTACGATCCGGCa            >  1:654031/1‑140 (MQ=255)

                                                                                                                           tCAACACCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCACGCAAGGTTTTAAACCACGGGTGACCATCTTTACGATCCGGCAGtt         <  2:754845/140‑1 (MQ=255)

                                                                                                                              acacCAGTGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCACGCAAGGTTTTAAACCACGGGTGACCATCTTTACGATCCGGCAGTTTac      >  2:639326/1‑140 (MQ=255)

                                                                                                                                  cagtgccagcgTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCACGCAAGGTTTTAAACCACGGGTGACCATCTTTACGATCCGGCAGTTTacgacg  <  2:626305/140‑1 (MQ=255)

TGGGCGGATCGTTCGCCAGCTCCGCTTCGACATGCTGCTCAATCATCAGCCCCTGACGCTGTTCGCTATCCAGAAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGTAACATCAAAACCAACATCAACACCAGCGCCAGCGTCAGCCAGAAGATGGCGAAGATGCGCGCGGTTAAGCTGCCTATCATGAAGCAGAAACCATCAGATAGCCGCGACCACGCAAGGTTTTAAACCACGGGTGACCATCTTTACGATCCGGCAGTTTACGACG  >  NC_000913/4104789‑4105058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A