Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,275,415	A→G	intergenic (‑374/‑56)	yjcB ← / → pdeC	uncharacterized protein YjcB/c‑di‑GMP phosphodiesterase PdeC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,275,415	0	A	G	100.0%	166.1 / NA	47	intergenic (‑374/‑56)	yjcB/pdeC	uncharacterized protein YjcB/c‑di‑GMP phosphodiesterase PdeC
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/21); total (26/21)

CCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCCATCATTCTTTCGC  >  NC_000913/4275278‑4275549

ccgccTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGcc                                                                                                                                      >  1:879843/1‑140 (MQ=255)

           ttCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCa                                                                                                                           >  1:877704/1‑140 (MQ=255)

              cTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCt                                                                                                                        >  1:794748/1‑140 (MQ=255)

                      aattaattTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATgg                                                                                                                >  2:633282/1‑140 (MQ=255)

                       attaattTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGc                                                                                                               <  2:877704/140‑1 (MQ=255)

                       attaattTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGc                                                                                                               <  1:251712/140‑1 (MQ=255)

                       attaattTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGc                                                                                                               <  1:296093/140‑1 (MQ=255)

                       attaattTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGc                                                                                                               <  1:695597/140‑1 (MQ=255)

                       attaattTTGAATATCAACGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGc                                                                                                               <  1:253499/140‑1 (MQ=255)

                        ttaattTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGcc                                                                                                              >  1:611742/1‑140 (MQ=255)

                                 aaTATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATaaa                                                                                                     <  1:301932/140‑1 (MQ=255)

                                 aaTATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGCTATcaa                                                                                                     >  1:225102/1‑140 (MQ=255)

                                        aTGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAAcaggcag                                                                                              <  2:209476/140‑1 (MQ=255)

                                         tGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAAcaggcagg                                                                                             <  2:451232/140‑1 (MQ=255)

                                             ttattTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAAt                                                                                         <  2:4545/140‑1 (MQ=255)

                                                          ggTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAg                                                                            <  2:879843/140‑1 (MQ=255)

                                                          ggTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAg                                                                            >  1:397793/1‑140 (MQ=255)

                                                                    aGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCa                                                                  <  1:232140/140‑1 (MQ=255)

                                                                         ggTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGaca                                                             <  1:633282/140‑1 (MQ=255)

                                                                          gTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGacac                                                            >  2:845995/1‑140 (MQ=255)

                                                                            aTCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGaca                                                             >  1:308501/1‑137 (MQ=255)

                                                                            aTCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGaca                                                             <  2:308501/137‑1 (MQ=255)

                                                                             tCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCaa                                                         >  1:64560/1‑140 (MQ=255)

                                                                             tCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCaa                                                         >  1:332593/1‑140 (MQ=255)

                                                                             tCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCaa                                                         >  2:503754/1‑140 (MQ=255)

                                                                                aTTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTa                                                      >  1:297721/1‑140 (MQ=255)

                                                                                aTTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTa                                                      >  1:57092/1‑140 (MQ=255)

                                                                                aTTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTa                                                      >  2:583884/1‑140 (MQ=255)

                                                                                     aaaTCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGc                                                 >  1:745672/1‑140 (MQ=255)

                                                                                         cAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTg                                             >  2:529971/1‑140 (MQ=255)

                                                                                           ggCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGcc                                           >  2:537777/1‑140 (MQ=255)

                                                                                           ggCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGcc                                           >  2:746544/1‑140 (MQ=255)

                                                                                               aaaGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGc                                       <  1:503754/140‑1 (MQ=255)

                                                                                                aaGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCa                                      <  1:583884/140‑1 (MQ=255)

                                                                                                    gCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTAt                                  <  1:537777/140‑1 (MQ=255)

                                                                                                             aCCGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGt                         >  2:730260/1‑140 (MQ=255)

                                                                                                              ccGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGtt                        <  2:745672/140‑1 (MQ=255)

                                                                                                               cGTAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTtc                       <  1:529971/140‑1 (MQ=255)

                                                                                                                 tAAGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTtctc                     >  1:570137/1‑140 (MQ=255)

                                                                                                                  aaGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTctct                    >  2:593083/1‑140 (MQ=255)

                                                                                                                  aaGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGCATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTctct                    >  2:763256/1‑140 (MQ=255)

                                                                                                                   aGATTTATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCtt                   <  2:570137/140‑1 (MQ=255)

                                                                                                                      tttATCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTcc                <  2:57092/140‑1 (MQ=255)

                                                                                                                         aTCTAAAGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCcat             >  2:469219/1‑140 (MQ=255)

                                                                                                                         aTCTAAAGACGTCGGTGCCCAGGGATTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCcat             >  1:154936/1‑140 (MQ=255)

                                                                                                                              aaGACGTCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCCATCATTc        <  1:746544/140‑1 (MQ=255)

                                                                                                                                    tCGGTGCCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCCATCATTCTTTCGc  >  2:542311/1‑140 (MQ=255)

CCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCCATCATTCTTTCGC  >  NC_000913/4275278‑4275549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A