Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F73 I1 R1	30	83.3	2909128	99.3%	2888764	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,708,678	C→A	noncoding (15/77 nt)	proK ←	tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,708,678	0	C	A	100.0%	115.7 / NA	33	noncoding (15/77 nt)	proK	tRNA‑Pro
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (21/12); total (21/12)

CCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCG  >  NC_000913/3708541‑3708812

ccTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATg                                                                                                                                      >  1:1126321/1‑140 (MQ=255)

         ggAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCa                                                                                                                             >  1:585425/1‑140 (MQ=255)

          gAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTAAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCAc                                                                                                                            >  1:559445/1‑140 (MQ=255)

                    tCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCggg                                                                                                                  <  2:585425/140‑1 (MQ=255)

                      cTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCggg                                                                                                                  >  1:13179/1‑138 (MQ=255)

                      cTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCggg                                                                                                                  <  2:13179/138‑1 (MQ=255)

                       tCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGgcg                                                                                                               >  1:428202/1‑140 (MQ=255)

                        cATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGgcgc                                                                                                              >  1:1323539/1‑140 (MQ=255)

                              cTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTa                                                                                                        >  1:1073433/1‑140 (MQ=255)

                                   gATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTgcg                                                                                                   <  2:428202/140‑1 (MQ=255)

                                     tGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCa                                                                                                 >  2:450949/1‑140 (MQ=255)

                                                   cTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGc                                                                                          <  1:49569/133‑1 (MQ=255)

                                                   cTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGc                                                                                          >  2:49569/1‑133 (MQ=255)

                                                      gTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAAt                                                                                <  2:1323539/140‑1 (MQ=255)

                                                             aGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTc                                                                                   >  2:835760/1‑130 (MQ=255)

                                                             aGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTc                                                                                   <  1:835760/130‑1 (MQ=255)

                                                                   ttCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCaa                                                                   <  2:559445/140‑1 (MQ=255)

                                                                         tttGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGcc                                                             >  2:1337068/1‑140 (MQ=255)

                                                                          ttGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCt                                                            >  1:305671/1‑140 (MQ=255)

                                                                                 gTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATc                                                     >  2:192204/1‑140 (MQ=255)

                                                                                     tAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCg                                                 <  2:1073433/140‑1 (MQ=255)

                                                                                            ttCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGt                                          >  2:972164/1‑140 (MQ=255)

                                                                                            ttCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGt                                          >  2:92504/1‑140 (MQ=255)

                                                                                                aaCCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTcagc                                      <  1:1337068/140‑1 (MQ=255)

                                                                                                    tCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGc                                  <  2:1393382/140‑1 (MQ=255)

                                                                                                    tCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGc                                  >  2:1181251/1‑140 (MQ=255)

                                                                                                    tCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAG‑AAGTCAGCAGGcc                                 >  1:938350/1‑140 (MQ=255)

                                                                                                                     gaacgaaGTGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCAGCTTAGTTAGCCGCTg                 <  1:192204/140‑1 (MQ=255)

                                                                                                                             tGCGCTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCtttt         >  2:205999/1‑140 (MQ=255)

                                                                                                                               cgcTACCAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGc       >  2:1117320/1‑140 (MQ=255)

                                                                                                                                    ccAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCg  <  2:1234742/140‑1 (MQ=255)

                                                                                                                                    ccAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCg  >  1:612450/1‑140 (MQ=255)

                                                                                                                                    ccAGGATGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCg  >  1:1234742/1‑140 (MQ=255)

CCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCG  >  NC_000913/3708541‑3708812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A