Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F73 I1 R1 30 83.3 2909128 99.3% 2888764 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,200,061 T→C G186G (GGT→GGC)  yjaG → conserved protein YjaG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,200,0610TC97.3% 264.9 / ‑3.1 73G186G (GGT→GGC) yjaGconserved protein YjaG
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (39/32);  total (40/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAGAGCTTAAAGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCG  >  NC_000913/4199927‑4200195
                                                                                                                                      |                                                                                                                                      
gAAGAGCTTAAAGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGagag                                                                                                                                   <  1:565274/140‑1 (MQ=255)
gAAGAGCTTAAAGAAAACCCAGCTGTAGAGCAAGAATGGAACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGagag                                                                                                                                   <  1:1051316/140‑1 (MQ=255)
 aaGAGCTTAAAGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGc                                                                                                                                  <  1:787856/140‑1 (MQ=255)
    agCTTAAAGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAat                                                                                                                               <  2:42710/140‑1 (MQ=255)
    agCTTAAAGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAat                                                                                                                               <  2:628115/140‑1 (MQ=255)
         aaaGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTgg                                                                                                                          >  1:729601/1‑140 (MQ=255)
         aaaGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTgg                                                                                                                          >  1:1408071/1‑140 (MQ=255)
         aaaGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTgg                                                                                                                          >  1:751759/1‑140 (MQ=255)
           aGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGta                                                                                                                        >  2:209577/1‑140 (MQ=255)
                 cccAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAatat                                                                                                                             >  1:929249/1‑129 (MQ=255)
                 cccAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAatat                                                                                                                             <  2:929249/129‑1 (MQ=255)
                  ccAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAAtttt                                                                                                                 <  1:550717/140‑1 (MQ=255)
                    aGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTc                                                                                                               >  2:1408254/1‑140 (MQ=255)
                          agagCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAAt                                                                                                         <  2:729601/140‑1 (MQ=255)
                            agCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATaa                                                                                                       <  1:382683/140‑1 (MQ=255)
                             gCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAg                                                                                                      <  2:228668/140‑1 (MQ=255)
                                aGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGAcc                                                                                                   >  1:746274/1‑140 (MQ=255)
                                aGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGAcc                                                                                                   >  2:190234/1‑140 (MQ=255)
                                 gAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCa                                                                                                  >  1:349906/1‑140 (MQ=255)
                                   aTGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGa                                                                                                >  1:1191009/1‑140 (MQ=255)
                                   aTGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGa                                                                                                >  2:380744/1‑140 (MQ=255)
                                      ggACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGaaaa                                                                                             >  2:939278/1‑140 (MQ=255)
                                        aCATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACg                                                                                           <  2:423613/140‑1 (MQ=255)
                                            tCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGAt                                                                                       >  2:544984/1‑140 (MQ=255)
                                            tCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGAt                                                                                       >  1:948604/1‑140 (MQ=255)
                                               gTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTa                                                                                    >  2:417555/1‑140 (MQ=255)
                                                       tatTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGa                                                                            <  2:753708/140‑1 (MQ=255)
                                                        atTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGAt                                                                           <  1:45750/140‑1 (MQ=255)
                                                          tCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGAttt                                                                         >  1:989983/1‑140 (MQ=255)
                                                            cGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGt                                                                       <  2:1298666/140‑1 (MQ=255)
                                                            cGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGt                                                                       <  2:1191009/140‑1 (MQ=255)
                                                            cGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGt                                                                       <  2:989983/140‑1 (MQ=255)
                                                            cGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGt                                                                       <  1:417555/140‑1 (MQ=255)
                                                            cGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGt                                                                       <  1:380744/140‑1 (MQ=255)
                                                            cGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGt                                                                       <  1:544984/140‑1 (MQ=255)
                                                             gACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGc                                                                                <  1:336354/130‑1 (MQ=255)
                                                             gACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGc                                                                                >  2:336354/1‑130 (MQ=255)
                                                              aCTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCg                                                                     <  1:939278/140‑1 (MQ=255)
                                                                          gTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGa                                                             >  2:1327066/1‑136 (MQ=255)
                                                                          gTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGa                                                             <  1:1327066/136‑1 (MQ=255)
                                                                           tGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCtt                                                        >  1:411095/1‑140 (MQ=255)
                                                                            gCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTc                                                       >  1:89354/1‑140 (MQ=255)
                                                                             cGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTcc                                                      <  2:349906/140‑1 (MQ=255)
                                                                                   aCGCGATATCGAGCTGATAAAAGGCCTTAGGGTAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCg                                                >  2:1235502/1‑140 (MQ=255)
                                                                                   aCGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCtt                                                        >  1:82072/1‑132 (MQ=255)
                                                                                   aCGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCtt                                                        <  2:82072/132‑1 (MQ=255)
                                                                                   aCGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCg                                                >  2:537282/1‑140 (MQ=255)
                                                                                     gcgATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCt                                                                >  1:1372105/1‑122 (MQ=255)
                                                                                     gcgATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCt                                                                <  2:1372105/122‑1 (MQ=255)
                                                                                           tCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGt                                        <  2:751759/140‑1 (MQ=255)
                                                                                            cGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTc                                       <  1:190234/140‑1 (MQ=255)
                                                                                            cGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTc                                       <  2:163723/140‑1 (MQ=255)
                                                                                             gAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCt                                      <  1:1441234/140‑1 (MQ=255)
                                                                                              aGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTg                                     >  1:874701/1‑140 (MQ=255)
                                                                                              aGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTg                                     >  2:129122/1‑140 (MQ=255)
                                                                                               gCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTgg                                    <  2:638978/140‑1 (MQ=255)
                                                                                                   aTAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAACACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTa                                >  2:657842/1‑140 (MQ=255)
                                                                                                                     gACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACTTTTGGGGGGGGaaaaa              >  2:1444214/1‑140 (MQ=255)
                                                                                                                     gACCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGaaaaa              >  2:115927/1‑140 (MQ=255)
                                                                                                                      aCCTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGaaaaaa             >  1:1325479/1‑140 (MQ=255)
                                                                                                                       ccTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTCCCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGaaaaaaa            <  2:656968/140‑1 (MQ=255)
                                                                                                                       ccTGCGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGaaaaaaa            >  2:1400597/1‑140 (MQ=255)
                                                                                                                           cGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg        >  1:1346921/1‑140 (MQ=255)
                                                                                                                           cGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg        >  2:697380/1‑140 (MQ=255)
                                                                                                                           cGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg        >  2:406315/1‑140 (MQ=255)
                                                                                                                           cGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg        >  2:566275/1‑140 (MQ=255)
                                                                                                                           cGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg        >  2:385947/1‑140 (MQ=255)
                                                                                                                           cGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg        >  2:268357/1‑140 (MQ=255)
                                                                                                                           cGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg        >  2:231192/1‑140 (MQ=255)
                                                                                                                           cGTGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGAgg        >  1:1196687/1‑140 (MQ=255)
                                                                                                                             tGAGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCt      >  1:140475/1‑140 (MQ=255)
                                                                                                                               aGGCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTAt    <  1:209577/140‑1 (MQ=255)
                                                                                                                                ggcgggcgAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATc   >  2:525372/1‑140 (MQ=255)
                                                                                                                                 gCGGGCGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCg  <  2:874701/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
GAAGAGCTTAAAGAAAACCCAGCTGTAGAGCAAGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCG  >  NC_000913/4199927‑4200195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A