Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F73 I1 R1 26 65.3 2245954 99.3% 2230232 139.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,341,904 C→A G213G (GGC→GGA)  kdsD → D‑arabinose 5‑phosphate isomerase KdsD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,341,9040CA100.0% 202.1 / NA 57G213G (GGC→GGA) kdsDD‑arabinose 5‑phosphate isomerase KdsD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/27);  total (30/27)

TGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGCATCTTTACCGACGGTGATTTAC  >  NC_000913/3341768‑3342040
                                                                                                                                        |                                                                                                                                        
tGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGAt                                                                                                                                       <  1:974987/140‑1 (MQ=255)
tGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGAt                                                                                                                                       <  2:12740/140‑1 (MQ=255)
       gggCGATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATcc                                                                                                                                <  2:33882/140‑1 (MQ=255)
        ggCGATGTCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATccc                                                                                                                               >  1:841687/1‑140 (MQ=255)
        ggCGATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATccc                                                                                                                               >  1:965241/1‑140 (MQ=255)
           gATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCa                                                                                                                            <  1:104379/140‑1 (MQ=255)
            aTGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCg                                                                                                                              >  1:761109/1‑137 (MQ=255)
            aTGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCg                                                                                                                              <  2:761109/137‑1 (MQ=255)
            aTGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCAt                                                                                                                           >  2:190301/1‑140 (MQ=255)
                         cgcgCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACgg                                                                                                              <  2:357435/140‑1 (MQ=255)
                          gcgcTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGc                                                                                                             <  2:594055/140‑1 (MQ=255)
                                ttAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCt                                                                                                       >  2:306324/1‑140 (MQ=255)
                                          gcgGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGAcgcg                                                                                             >  1:17784/1‑140 (MQ=255)
                                          gcgGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGAcgcg                                                                                             >  1:362149/1‑140 (MQ=255)
                                          gcgGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGAcgcg                                                                                             <  2:971606/140‑1 (MQ=255)
                                              cTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGTCAGTCTGCGTGACGCGTTGc                                                                                         <  1:255140/140‑1 (MQ=255)
                                              cTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGc                                                                                         <  1:364372/140‑1 (MQ=255)
                                                      ctgAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGtt                                                                                 >  1:679761/1‑140 (MQ=255)
                                                         aaGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTAcc                                                                              <  2:362149/140‑1 (MQ=255)
                                                         aaGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTAcc                                                                              <  1:965893/140‑1 (MQ=255)
                                                                  cgcTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAAt                                                                     <  2:996432/140‑1 (MQ=255)
                                                                   gcTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATc                                                                    <  2:965241/140‑1 (MQ=255)
                                                                   gcTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATc                                                                    <  1:242424/140‑1 (MQ=255)
                                                                       tcACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTgg                                                                >  2:1093089/1‑140 (MQ=255)
                                                                               aGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTg                                                        >  1:195788/1‑140 (MQ=255)
                                                                                 gcggcgCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTc                                                      >  1:146054/1‑140 (MQ=255)
                                                                                 gcggcgCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTc                                                      >  1:899651/1‑140 (MQ=255)
                                                                                 gcggcgCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTc                                                      >  2:101798/1‑140 (MQ=255)
                                                                                  gggcgCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCa                                                     <  2:206680/139‑1 (MQ=255)
                                                                                  cggcgCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCa                                                     <  2:945525/140‑1 (MQ=255)
                                                                                     cgcACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCAttt                                                  >  2:119916/1‑140 (MQ=255)
                                                                                      gcACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTg                                                 >  1:931437/1‑140 (MQ=255)
                                                                                         cTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGa                                              <  2:931437/140‑1 (MQ=255)
                                                                                         cTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGa                                              <  1:1093089/140‑1 (MQ=255)
                                                                                          tGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGAt                                             <  2:272120/140‑1 (MQ=255)
                                                                                          tGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGAt                                             <  2:679761/140‑1 (MQ=255)
                                                                                           gggTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATg                                            >  2:658036/1‑140 (MQ=255)
                                                                                             gTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGAc                                          <  1:119916/140‑1 (MQ=255)
                                                                                              tCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACa                                         <  1:306324/140‑1 (MQ=255)
                                                                                               cGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACaa                                        >  1:158189/1‑140 (MQ=255)
                                                                                                  aaaCTTCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAatat                                     <  1:101798/140‑1 (MQ=255)
                                                                                                      ttCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATatgatg                                 >  2:853196/1‑140 (MQ=255)
                                                                                                       tCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATAtgatga                                <  1:874699/140‑1 (MQ=255)
                                                                                                       tCTGCTGCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATAtgatga                                >  2:951912/1‑140 (MQ=255)
                                                                                                        ctgctgCGCGTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATgatgat                               >  2:690907/1‑140 (MQ=255)
                                                                                                              cgcgTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACaa                                        <  2:345095/125‑1 (MQ=255)
                                                                                                              cgcgTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACaa                                        >  1:345095/1‑125 (MQ=255)
                                                                                                              cgcgTAAACGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAgg                         >  1:31810/1‑140 (MQ=255)
                                                                                                                     aCGATATTATGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGCATCttt                  >  1:545148/1‑140 (MQ=255)
                                                                                                                            tatGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGCATCTTTACCGACg           >  2:517636/1‑140 (MQ=255)
                                                                                                                             atGCATACGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGCATCTTTACCGACgg          >  1:38630/1‑140 (MQ=255)
                                                                                                                                   aCGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGCATCTTTACCGACg           <  2:567069/133‑1 (MQ=255)
                                                                                                                                   aCGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGCATCTTTACCGACg           >  1:567069/1‑133 (MQ=255)
                                                                                                                                   aCGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGCATCTTTACCGACGGTGAttt    >  2:389748/1‑140 (MQ=255)
                                                                                                                                   aCGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGCATCTTTACCGACGGTGAttt    >  1:565405/1‑140 (MQ=255)
                                                                                                                                   aCGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGCATCTTCACCGACGGTGAttt    >  2:836977/1‑140 (MQ=255)
                                                                                                                                   aCGGGAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTCGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGCATCTTTACCGACGGTGAttt    >  1:937779/1‑140 (MQ=255)
                                                                                                                                     gggAGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGCATCTTTACCGACGGTGATTTAc  >  2:275856/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGCATCTTTACCGACGGTGATTTAC  >  NC_000913/3341768‑3342040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A