Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F73 I1 R1	26	65.3	2245954	99.3%	2230232	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,393,908	C→A	G784V (GGT→GTT)	yhdP ←	AsmA2 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,393,908	0	C	A	97.4%	130.2 / ‑2.8	39	G784V (GGT→GTT)	yhdP	AsmA2 domain‑containing protein
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (22/16); total (23/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.90e-01

TAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTACCTGGT  >  NC_000913/3393772‑3394043

tAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAg                                                                                                                                      <  1:387430/140‑1 (MQ=255)

     cAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGa                                                                                                                                 <  1:927211/140‑1 (MQ=255)

         gCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAg                                                                                                                             <  1:342648/140‑1 (MQ=255)

                    tGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATg                                                                                                                  <  2:391690/140‑1 (MQ=255)

                    tGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATg                                                                                                                  <  2:353563/140‑1 (MQ=255)

                       cATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGccc                                                                                                               >  2:88675/1‑140 (MQ=255)

                          cAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACt                                                                                                            >  2:517495/1‑140 (MQ=255)

                                       tACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCa                                                                                                              <  2:72203/125‑1 (MQ=255)

                                       tACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCa                                                                                                              >  1:72203/1‑125 (MQ=255)

                                        aCCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCccacgc                                                                                              <  1:88675/140‑1 (MQ=255)

                                        aCCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCccacgc                                                                                              >  1:612654/1‑140 (MQ=255)

                                            cTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCccacgccacg                                                                                          <  1:517495/140‑1 (MQ=255)

                                            cTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCccacgccacg                                                                                          <  2:612654/140‑1 (MQ=255)

                                                             ggTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTc                                                                         >  1:495763/1‑140 (MQ=255)

                                                                ttGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGtt                                                                      >  2:470594/1‑140 (MQ=255)

                                                                  ggCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCa                                                                    >  1:580212/1‑140 (MQ=255)

                                                                             aaGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGc                                                         >  1:880941/1‑140 (MQ=255)

                                                                                    gTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGc                                                  >  1:748846/1‑140 (MQ=255)

                                                                                         tGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGtt                                             >  1:975001/1‑140 (MQ=255)

                                                                                          gCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGttt                                            >  1:381523/1‑140 (MQ=255)

                                                                                                         aTCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGt                             <  2:748846/140‑1 (MQ=255)

                                                                                                          tCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGtt                            >  1:119861/1‑140 (MQ=255)

                                                                                                          tCGCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGtt                            >  2:366317/1‑140 (MQ=255)

                                                                                                            gCCGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTAc                          >  2:708888/1‑140 (MQ=255)

                                                                                                              cGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCg                        >  1:410748/1‑140 (MQ=255)

                                                                                                              cGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCg                        >  2:787874/1‑140 (MQ=255)

                                                                                                              cGTTCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCg                        <  2:832326/140‑1 (MQ=255)

                                                                                                                ttCAGCTCTATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGtt                      <  1:787874/140‑1 (MQ=255)

                                                                                                                      tctATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGtt                <  1:366317/140‑1 (MQ=255)

                                                                                                                        tATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTa              >  2:216696/1‑140 (MQ=255)

                                                                                                                        tATGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTa              <  1:470594/140‑1 (MQ=255)

                                                                                                                          tGTTATAGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTAcc            >  2:362708/1‑140 (MQ=255)

                                                                                                                             tataGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCt         <  2:881069/140‑1 (MQ=255)

                                                                                                                               taGGTCGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTAc       >  1:853775/1‑140 (MQ=255)

                                                                                                                                    cGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACACTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGCTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTACCTGGt  >  2:210362/1‑140 (MQ=255)

                                                                                                                                    cGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTa        >  2:525366/1‑134 (MQ=255)

                                                                                                                                    cGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTa        <  1:525366/134‑1 (MQ=255)

                                                                                                                                    cGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTACCTGGt  >  1:207361/1‑140 (MQ=255)

                                                                                                                                    cGCAACAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTACCTGGt  >  2:254925/1‑140 (MQ=255)

TAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTACCTGGT  >  NC_000913/3393772‑3394043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A