Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,151,093	A→G	T142A (ACC→GCC)	fabG →	3‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,151,093	0	A	G	100.0%	179.8 / NA	48	T142A (ACC→GCC)	fabG	3‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/22); total (26/22)

GCGAATGAAAGATGAAGAGTGGAACGATATTATCGAAACCAACCTTTCATCTGTTTTCCGTCTGTCAAAAGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGA  >  NC_000913/1150957‑1151229

gCGAATGAAAGATGAAGAGTGGAACGATATTATCGAAACCAACCTTTCATCTGTTTTCCGTCTGTCAAAAGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCa                                                                                                                                       >  1:955583/1‑140 (MQ=255)

gCGAATGAAAGATGAAGAGTGGAACGATATTATCGAAACCAACCTTTCATCTGTTTTCCGTCTGTCAAAAGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCa                                                                                                                                       >  2:421258/1‑140 (MQ=255)

     tGAAAGATGAAGAGTGGAACGATATTATCGAAACCAACCTTTCATCTGTTTTCCGTCTGTCAAAAGCGGTAATGCGTGCTATGATGACAAATCGTCATGGTCGTATTATCACTATCGGTTCTGTTGTTGGTGCCATTGGa                                                                                                                                  >  2:204821/1‑140 (MQ=255)

              aaGAGTGGAACGATATTATCGAAACCAACCTTTCATCTGTTTTCCGTCTGTCAAAAGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGt                                                                                                                         <  2:955583/140‑1 (MQ=255)

              aaGAGTGGAACGATATTATCGAAACCAACCTTTCATCTGTTTTCCGTCTGTCAAAAGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGt                                                                                                                         <  1:405905/140‑1 (MQ=255)

               agagTGGAACGATATTATCGAAACCAACCTTTCATCTGTTTTCCGTCTGTCAAAAGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTc                                                                                                                        <  1:133836/140‑1 (MQ=255)

               agagTGGAACGATATTATCGAAACCAACCTTTCATCTGTTTTCCGTCTGTCAAAAGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTc                                                                                                                        <  2:698307/140‑1 (MQ=255)

                             ttatCGAAACCAACCTTTCATCTGTTTTCCGTCTGTCAAAAGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCt                                                                                                          >  2:446663/1‑140 (MQ=255)

                                  gAAACCAACCTTTCATCTGTTTTCCGTCTGTCAAAAGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTgcggc                                                                                                     <  1:868512/140‑1 (MQ=255)

                                   aaaCCAACCTTTCATCTGTTTTCCGTCTGTCAAAAGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTgcggcg                                                                                                    >  1:545848/1‑140 (MQ=255)

                                     accaaccTTTCATCTGTTTTCCGTCTGTCAAAAGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGaa                                                                                                  >  2:785099/1‑140 (MQ=255)

                                            tttCATCTGTTTTCCGTCTGTCAAAAGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGc                                                                                           <  1:598213/140‑1 (MQ=255)

                                              tCATCTGTTTTCCGTCTGTCAAAAGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGcct                                                                                         >  2:110752/1‑138 (MQ=255)

                                                          cGTCTGTCAAAAGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAg                                                                             <  2:700767/140‑1 (MQ=255)

                                                            tCTGTCAAAAGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTa                                                                           >  2:346839/1‑140 (MQ=255)

                                                            tCTGTCAAAAGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTa                                                                           >  2:955026/1‑140 (MQ=255)

                                                             cTGTCAAAAGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGGAAGCGGGCTTGATCGGCCTCCGTaa                                                                          >  2:708828/1‑140 (MQ=255)

                                                                   aaaGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACt                                                                    <  2:245331/140‑1 (MQ=255)

                                                                          tAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGgcgcgc                                                             >  2:734237/1‑140 (MQ=255)

                                                                                    tATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGt                                                   <  2:307497/140‑1 (MQ=255)

                                                                                        atgaAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCAcg                                               <  1:737807/140‑1 (MQ=255)

                                                                                        atgaAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCAcg                                               <  1:23478/140‑1 (MQ=255)

                                                                                        atgaAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCAcg                                               <  2:346162/140‑1 (MQ=255)

                                                                                        atgaAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCAcg                                               <  2:15582/140‑1 (MQ=255)

                                                                                          gaAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCAcgcg                                             <  2:545848/140‑1 (MQ=255)

                                                                                             aaaGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTa                                          <  1:785099/140‑1 (MQ=255)

                                                                                                gCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTa                                       >  1:254916/1‑140 (MQ=255)

                                                                                                gCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTa                                       >  1:981402/1‑140 (MQ=255)

                                                                                                gCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGGATTa                                       >  1:633363/1‑140 (MQ=255)

                                                                                                gCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGGATTa                                       >  2:906120/1‑140 (MQ=255)

                                                                                                     aTGGTCGTATTATCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGTGTCACCCGGTATTACTGTa                                  >  2:766957/1‑140 (MQ=255)

                                                                                                              ttatCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGGATTACTGTAAACggtggt                         >  2:1037582/1‑138 (MQ=255)

                                                                                                                 tCACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCt                      <  1:346839/140‑1 (MQ=255)

                                                                                                                  cACTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTc                     >  1:697918/1‑140 (MQ=255)

                                                                                                                   aCTATCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTcc                    >  2:57965/1‑140 (MQ=255)

                                                                                                                       tCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGc                >  1:521921/1‑140 (MQ=255)

                                                                                                                       tCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGc                >  1:953245/1‑140 (MQ=255)

                                                                                                                       tCGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGc                >  1:561246/1‑140 (MQ=255)

                                                                                                                        cGGTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCt               <  2:695792/140‑1 (MQ=255)

                                                                                                                         ggTTCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCtt              <  2:365533/140‑1 (MQ=255)

                                                                                                                           ttCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTa            >  1:384701/1‑140 (MQ=255)

                                                                                                                           ttCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTa            >  1:376577/1‑140 (MQ=255)

                                                                                                                           ttCTGTGGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCCTGATCGGCTTCAGTAAATCACTGGCTAGCGAAGTTGCGTCACCCGGGATTACTGTAAACGGTAGTGCTCCGGGCTTTa            >  2:960454/1‑140 (MQ=255)

                                                                                                                               gtgGTTGGTGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGa        >  1:163199/1‑140 (MQ=255)

                                                                                                                                tggttggtGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGaa       >  2:342089/1‑140 (MQ=255)

                                                                                                                                tggttggtGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGaa       <  1:633088/140‑1 (MQ=255)

                                                                                                                                 ggttggtGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTTACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGaaa      <  1:421258/140‑1 (MQ=255)

                                                                                                                                   ttggtGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACg    <  1:708828/140‑1 (MQ=255)

                                                                                                                                   ttggtGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACg    <  1:696740/140‑1 (MQ=255)

                                                                                                                                     ggtGCCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCCGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGa  <  2:1003596/140‑1 (MQ=255)

GCGAATGAAAGATGAAGAGTGGAACGATATTATCGAAACCAACCTTTCATCTGTTTTCCGTCTGTCAAAAGCGGTAATGCGCGCTATGATGAAAAAGCGTCATGGTCGTATTATCACTATCGGTTCTGTGGTTGGTACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGA  >  NC_000913/1150957‑1151229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A