Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,244,726	A→G	intergenic (‑199/‑2)	ycgR ← / → ymgE	flagellar brake protein YcgR/PF04226 family protein YmgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,244,726	0	A	G	100.0%	201.5 / NA	53	intergenic (‑199/‑2)	ycgR/ymgE	flagellar brake protein YcgR/PF04226 family protein YmgE
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (34/19); total (34/19)

ATACAAATGCGTGAATATTTTTTACATGTTGTTCTTAAATCAGCCGACATACGCCCAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGG  >  NC_000913/1244589‑1244858

aTACAAATGCGTGAATATTTTTTACATGTTGTTCTTAAATCAGCCGACATACGCCCAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCa                                                                                                                                    >  2:976498/1‑140 (MQ=255)

             aaTATTTTTTACATGTTGTTCTTAAATCAGCCGACATACGCCCAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGc                                                                                                                       <  2:568043/140‑1 (MQ=255)

                   ttttACATGTTGTTCTTAAATCAGCCGACATACGCCCAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGat                                                                                                                 <  1:896587/140‑1 (MQ=255)

                        cATGTTGTTCTTAAATCAGCCGACATACGCCCAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTAttt                                                                                                            >  2:542474/1‑140 (MQ=255)

                         aTGTTGTTCTTAAATCAGCCGACATACGCCCAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTAtttt                                                                                                           <  1:901663/140‑1 (MQ=255)

                          tgttgtTCTTAAATCAGCCGACATACGCCCAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTAttttt                                                                                                          >  2:539701/1‑140 (MQ=255)

                             tgttCTTAAATCAGCCGACATACGCCCAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGAc                                                                                                       >  1:714904/1‑140 (MQ=255)

                                 cTTAAATCAGCCGACATACGCCCAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGa                                                                                                   >  2:1003854/1‑140 (MQ=255)

                                    aaaTCAGCCGACATACGCCCAGCATCGATCCCGTCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAg                                                                                                >  2:72361/1‑140 (MQ=255)

                                     aaTCAGCCGACATACGCCCAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGc                                                                                               <  1:858644/140‑1 (MQ=255)

                                       tCAGCCGACATACGCCCAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCg                                                                                             >  2:366248/1‑140 (MQ=255)

                                         aGCCGACATACGCCCAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGc                                                                                           >  1:523251/1‑140 (MQ=255)

                                         aGCCGACATACGCCCAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGc                                                                                           >  2:433235/1‑140 (MQ=255)

                                         aGCCGACATACGCCCAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGCAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGTATTATTGCATGGATTATTTTTGACCTGAAAGCCGGc                                                                                           >  2:711015/1‑140 (MQ=255)

                                           ccGACATACGCCCAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCAt                                                                                         >  2:493765/1‑140 (MQ=255)

                                            cGACATACGCCCAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCAtt                                                                                        <  1:131544/140‑1 (MQ=255)

                                                   cGCCCAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCa                                                                                 >  1:356500/1‑140 (MQ=255)

                                                     cccAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAg                                                                               >  2:567138/1‑140 (MQ=255)

                                                     cccAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAg                                                                               <  1:150235/140‑1 (MQ=255)

                                                      ccAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGc                                                                              >  2:993273/1‑140 (MQ=255)

                                                         gCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTaa                                                                           >  1:871928/1‑140 (MQ=255)

                                                         gCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTaa                                                                           >  1:423038/1‑140 (MQ=255)

                                                              gATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATg                                                                      >  1:772655/1‑140 (MQ=255)

                                                                tCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGcc                                                                    <  1:366248/140‑1 (MQ=255)

                                                                    gCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCgggg                                                                >  1:87541/1‑140 (MQ=255)

                                                                    gCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCgggg                                                                >  1:464316/1‑140 (MQ=255)

                                                                         tGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGa                                                           <  1:976498/140‑1 (MQ=255)

                                                                         tGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGa                                                           <  2:54836/140‑1 (MQ=255)

                                                                          gCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGAt                                                          >  1:866126/1‑140 (MQ=255)

                                                                              ataCTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGAtggtg                                                      <  2:655852/140‑1 (MQ=255)

                                                                                    agagCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGAt                                                >  1:540145/1‑140 (MQ=255)

                                                                                    agagCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGAt                                                >  1:19990/1‑140 (MQ=255)

                                                                                    agagCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGAt                                                >  1:502890/1‑140 (MQ=255)

                                                                                    agagCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGAt                                                <  2:431902/140‑1 (MQ=255)

                                                                                    agagCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGAt                                                >  1:872300/1‑140 (MQ=255)

                                                                                    agagCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGAt                                                <  1:756172/140‑1 (MQ=255)

                                                                                       gCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGAtttt                                             >  1:860463/1‑140 (MQ=255)

                                                                                         gTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTc                                           <  1:329987/140‑1 (MQ=255)

                                                                                          ttGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTcc                                          >  1:646583/1‑140 (MQ=255)

                                                                                          ttGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTcc                                          >  1:788613/1‑140 (MQ=255)

                                                                                          ttGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTcc                                          >  1:383745/1‑140 (MQ=255)

                                                                                            gATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTg                                        <  2:356500/140‑1 (MQ=255)

                                                                                            gATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGAGGATTTTTCCTg                                        >  1:816023/1‑140 (MQ=255)

                                                                                                tAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCt                                    >  2:959940/1‑140 (MQ=255)

                                                                                                       ttCTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATtct                             >  2:894862/1‑140 (MQ=255)

                                                                                                         cTTGCGTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCg                           <  1:433235/140‑1 (MQ=255)

                                                                                                              gTCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATa                      >  1:754171/1‑140 (MQ=255)

                                                                                                               tCCGATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAg                     >  2:28688/1‑140 (MQ=255)

                                                                                                                  gATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTcg                  <  2:464316/140‑1 (MQ=255)

                                                                                                                  gATTCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTcg                  <  2:87541/140‑1 (MQ=255)

                                                                                                                     tCATCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTcggtg               <  2:772655/140‑1 (MQ=255)

                                                                                                                        tCGAAACGAGGGCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTcggtgcgg            <  2:383745/140‑1 (MQ=255)

                                                                                                                                 gggCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTcggcg   >  2:915258/1‑140 (MQ=255)

                                                                                                                                  ggCCTGAGCATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTcggcgg  >  1:811285/1‑140 (MQ=255)

ATACAAATGCGTGAATATTTTTTACATGTTGTTCTTAAATCAGCCGACATACGCCCAGCATCGATCCCGCCCTTGCCTATACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGG  >  NC_000913/1244589‑1244858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A