Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,270,719	(C)_5→6	intergenic (+259/+30)	rdlC → / ← chaA	small regulatory antisense RNA RdlC/Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,270,714	1	.	C	100.0%	151.3 / NA	41	intergenic (+254/+35)	rdlC/chaA	small regulatory antisense RNA RdlC/Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (19/22); total (19/22)

TTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCT‑CCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACA  >  NC_000913/1270575‑1270846

tttGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTAtct                                                                                                                                       >  2:340286/1‑140 (MQ=255)

tttGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGGACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTAtct                                                                                                                                       >  2:234721/1‑140 (MQ=255)

   gCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCT‑ccc                                                                                                                                   <  1:758553/140‑1 (MQ=255)

   gCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCT‑ccc                                                                                                                                   >  1:56906/1‑140 (MQ=255)

   gCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCT‑ccc                                                                                                                                   <  2:532620/140‑1 (MQ=255)

   gCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGGTGCAGGGGTTTCGGGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGGCCTGGCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCT‑ccc                                                                                                                                   <  2:595938/140‑1 (MQ=39)

                  ttCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCAt                                                                                                                     <  2:163604/140‑1 (MQ=255)

                  ttCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCAt                                                                                                                     <  1:315582/140‑1 (MQ=255)

                  ttCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCAt                                                                                                                     <  1:221021/140‑1 (MQ=255)

                   tCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATc                                                                                                                    >  1:273745/1‑140 (MQ=255)

                   tCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATc                                                                                                                    >  2:770780/1‑140 (MQ=255)

                    ccGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCg                                                                                                                   <  1:648025/140‑1 (MQ=255)

                      ggCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCgga                                                                                                                 <  1:980139/140‑1 (MQ=255)

                       gCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCggag                                                                                                                <  2:797251/140‑1 (MQ=255)

                        cTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCggagg                                                                                                               <  2:765568/140‑1 (MQ=255)

                        cTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCggagg                                                                                                               <  1:43926/140‑1 (MQ=255)

                        cTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCggagg                                                                                                               <  2:600250/140‑1 (MQ=255)

                        cTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCggagg                                                                                                               >  2:45891/1‑140 (MQ=255)

                        cTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCggagg                                                                                                               <  2:836089/140‑1 (MQ=255)

                                     gTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTc                                                                                                  >  2:181284/1‑140 (MQ=255)

                                              gTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAata                                                                                         >  1:428294/1‑140 (MQ=255)

                                                            gtgGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAg                                                                           <  2:46795/140‑1 (MQ=255)

                                                                   aGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCaa                                                                    >  2:12675/1‑140 (MQ=255)

                                                                     gCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCaaaa                                                                  >  2:661102/1‑139 (MQ=255)

                                                                     gCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAAc                                                                  >  2:1024722/1‑140 (MQ=255)

                                                                       tCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAg                                                                >  1:53040/1‑140 (MQ=255)

                                                                       tCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAg                                                                >  2:448297/1‑140 (MQ=255)

                                                                        cGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGt                                                               >  1:731708/1‑140 (MQ=255)

                                                                        cGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGt                                                               <  2:56906/140‑1 (MQ=255)

                                                                          cAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCTGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGc                                                             >  2:531791/1‑140 (MQ=255)

                                                                             ttCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAg                                                          <  1:154477/140‑1 (MQ=255)

                                                                              tCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGa                                                         <  2:506339/140‑1 (MQ=255)

                                                                               cTGATTAACAACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAt                                                        >  2:728682/1‑140 (MQ=255)

                                                                                     aaCGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAtgcgctg                                                  <  1:45891/140‑1 (MQ=255)

                                                                                              tCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGa                                         >  1:317368/1‑140 (MQ=255)

                                                                                               ccAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAg                                        <  1:12675/140‑1 (MQ=255)

                                                                                                        aTGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGt                               >  1:794650/1‑140 (MQ=255)

                                                                                                                 tACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGt                      >  1:723978/1‑140 (MQ=255)

                                                                                                                 tACAATAAGCAGGAATTTATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGt                      >  2:756453/1‑140 (MQ=255)

                                                                                                                                 ttATATTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATgg      <  1:448297/140‑1 (MQ=255)

                                                                                                                                  tataTTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGc     >  2:24908/1‑140 (MQ=255)

                                                                                                                                   atatTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGca    <  1:1024722/140‑1 (MQ=255)

                                                                                                                                   atatTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGca    <  2:53040/140‑1 (MQ=255)

                                                                                                                                   atatTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGca    <  1:233550/140‑1 (MQ=255)

                                                                                                                                   atatTATCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGca    <  2:428294/140‑1 (MQ=255)

                                                                                                                                    tattatCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGtac   >  1:511337/1‑140 (MQ=255)

                                                                                                                                     attatCTCCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTTCGGTGGAGAAGGAGATATGGcaca  <  1:440489/140‑1 (MQ=255)

TTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCT‑CCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACA  >  NC_000913/1270575‑1270846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A