Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,405,392	A→G	T218A (ACG→GCG)	abgR →	putative LysR‑type DNA‑binding transcriptional regulator AbgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,405,392	0	A	G	100.0%	235.9 / NA	63	T218A (ACG→GCG)	abgR	putative LysR‑type DNA‑binding transcriptional regulator AbgR
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/35); total (28/35)

TCTTTTGCCGCCCGGGACACCCCGCCATTGGTGCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGG  >  NC_000913/1405258‑1405527

tCTTTTGCCGCCCGGGACACCCCGCCATTGGTGCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCca                                                                                                                                    <  1:226594/140‑1 (MQ=255)

  ttttGCCGCCCGGGACACCCCGCCATTGGTGCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCcaca                                                                                                                                  <  1:79237/140‑1 (MQ=255)

    ttGCCGCCCGGGACACCCCGCCATTGGTGCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAgg                                                                                                                                <  2:821057/140‑1 (MQ=255)

    ttGCCGCCCGGGACACCCCGCCATTGGTGCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAgg                                                                                                                                <  1:461621/140‑1 (MQ=255)

     tGCCGCCCGGGACACCCCGCCATTGGTGCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGt                                                                                                                               <  2:343714/140‑1 (MQ=255)

     tGCCGCCCGGGACACCCCGCCATTGGTGCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGt                                                                                                                               <  2:1039787/140‑1 (MQ=255)

     tGCCGCCCGGGACACCCCGCCATTGGTGCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGt                                                                                                                               <  2:144530/140‑1 (MQ=255)

          cccGGGACACCCCGCCATTGGTGCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGgtg                                                                                                                          >  2:376442/1‑140 (MQ=255)

                    cccGCCATTGGTGCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCga                                                                                                                >  1:880774/1‑140 (MQ=255)

                    cccGCCATTGGTGCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCga                                                                                                                >  1:296869/1‑140 (MQ=255)

                            tGGTGCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTtct                                                                                                        <  2:410070/140‑1 (MQ=255)

                            tGGTGCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTtct                                                                                                        <  2:5020/140‑1 (MQ=255)

                            tGGTGCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTtct                                                                                                        <  1:65519/140‑1 (MQ=255)

                              gTGCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCa                                                                                                      >  1:129830/1‑140 (MQ=255)

                               tGCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAg                                                                                                     <  2:857686/140‑1 (MQ=255)

                                gCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTTCAAACAGTTGAGGGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGc                                                                                                    <  1:297946/140‑1 (MQ=255)

                                gCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGc                                                                                                    <  1:419316/140‑1 (MQ=255)

                                gCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGc                                                                                                    <  2:905929/140‑1 (MQ=255)

                                gCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGc                                                                                                    <  2:492960/140‑1 (MQ=255)

                                    gTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGt                                                                                                <  1:189160/140‑1 (MQ=255)

                                             aaCAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCtg                                                                                       <  2:921782/140‑1 (MQ=255)

                                              aCAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCtgg                                                                                      >  2:612312/1‑140 (MQ=255)

                                                 gTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCtggtgg                                                                                   <  1:619319/140‑1 (MQ=255)

                                                    aCTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAACCAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCaa                                                                                >  2:154399/1‑140 (MQ=255)

                                                    aCTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCaa                                                                                >  2:801571/1‑140 (MQ=255)

                                                    aCTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCaa                                                                                >  2:830193/1‑140 (MQ=255)

                                                    aCTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCaa                                                                                >  2:371864/1‑140 (MQ=255)

                                                          ttACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTGGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCg                                                                          >  2:737473/1‑140 (MQ=255)

                                                            aCAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGAt                                                                        >  2:599634/1‑140 (MQ=255)

                                                            aCAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGAt                                                                        >  2:788079/1‑140 (MQ=255)

                                                            aCAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGAt                                                                        >  2:964709/1‑140 (MQ=255)

                                                              aGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGAttt                                                                      <  2:296869/140‑1 (MQ=255)

                                                              aGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGAttt                                                                      <  2:880774/140‑1 (MQ=255)

                                                                  ggACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTtctc                                                                  >  2:309007/1‑140 (MQ=255)

                                                                  ggACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTtctc                                                                  >  2:928675/1‑140 (MQ=255)

                                                                  ggACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTtctc                                                                  >  2:432569/1‑140 (MQ=255)

                                                                   gACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCa                                                                 <  1:376442/140‑1 (MQ=255)

                                                                   gACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCa                                                                 >  2:776480/1‑140 (MQ=255)

                                                                   gACAATGCCGACACCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCa                                                                 >  1:204152/1‑140 (MQ=255)

                                                                        tGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCaaa                                                            <  1:375206/140‑1 (MQ=255)

                                                                        tGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCaaa                                                            <  2:550737/140‑1 (MQ=255)

                                                                        tGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCaaa                                                            >  1:611830/1‑140 (MQ=255)

                                                                             aCGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGcc                                                       >  2:804033/1‑140 (MQ=255)

                                                                                  acacGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTgcag                                                  >  1:45041/1‑140 (MQ=255)

                                                                                   cacGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTgaaga                                                 >  2:505969/1‑140 (MQ=255)

                                                                                    acGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTgaagaa                                                >  2:716546/1‑140 (MQ=255)

                                                                                        cAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATgg                                            >  1:739512/1‑140 (MQ=255)

                                                                                              ctacAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCg                                      <  1:197995/140‑1 (MQ=255)

                                                                                                acAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGAt                                    <  1:801571/140‑1 (MQ=255)

                                                                                                  aaaCAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATcc                                  <  1:964709/140‑1 (MQ=255)

                                                                                                     cAGTTGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCtt                               >  2:596268/1‑140 (MQ=255)

                                                                                                        ttGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCa                            >  2:864270/1‑140 (MQ=255)

                                                                                                         tGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATcc                                  <  1:724109/133‑1 (MQ=255)

                                                                                                         tGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATcc                                  >  2:724109/1‑133 (MQ=255)

                                                                                                         tGAGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCAc                           <  1:309007/140‑1 (MQ=255)

                                                                                                           aGTGAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACgg                         <  1:154399/140‑1 (MQ=255)

                                                                                                              gAATTGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACa                      <  2:298110/140‑1 (MQ=255)

                                                                                                                 ttGCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAggg                   <  1:370056/140‑1 (MQ=255)

                                                                                                                   gCTTGACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGc                 <  2:421970/140‑1 (MQ=255)

                                                                                                                       gACGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGt             <  1:612312/140‑1 (MQ=255)

                                                                                                                         cGATCAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGa           <  1:432569/140‑1 (MQ=255)

                                                                                                                             cAGGCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGtt       >  1:947017/1‑140 (MQ=255)

                                                                                                                               ggCGCAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGc     >  2:1010063/1‑140 (MQ=255)

                                                                                                                                  gcAAGCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCgg  <  1:928675/140‑1 (MQ=255)

TCTTTTGCCGCCCGGGACACCCCGCCATTGGTGCCCGTTCGATCAAACAGTTACTGGATTACAGCTGGACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGG  >  NC_000913/1405258‑1405527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A