Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,542,969	A→G	F187S (TTC→TCC)	cysP ←	thiosulfate/sulfate ABC transporter periplasmic binding protein CysP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,542,969	0	A	G	100.0%	154.4 / NA	42	F187S (TTC→TCC)	cysP	thiosulfate/sulfate ABC transporter periplasmic binding protein CysP
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/14); total (28/14)

TGCGCTTCATACTGTTTACGGATGTTGTTCACTTCCGATTCGAAGCTAATCAGCACATCGCCCAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGAACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATAACGCGCGTTACCCGACGTTTTCGGGTTCGGGAAAATCAGCTTCACGTCGGAG  >  NC_000913/2542837‑2543103

tGCGCTTCATACTGTTTACGGATGTTGTTCACTTCCGATTCGAAGCTAATCAGCACATCGCCCAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGt                                                                                                                                 >  2:858495/1‑140 (MQ=255)

  cgcTTCATACTGTTTACGGATGTTGTTCACTTCCGATTCGAAGCTAATCAGCACATCGCCCAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCa                                                                                                                               >  2:616975/1‑140 (MQ=255)

       cATACTGTTTACGGATGTTGTTCACTTCCGATTCGAAGCTAATCAGCACATCGCCCAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAAc                                                                                                                          >  2:790293/1‑140 (MQ=255)

                 aCGGATGTTGTTCACTTCCGATTCGAAGCTAATCAGCACATCGCCCAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGttt                                                                                                                >  2:325067/1‑140 (MQ=255)

                 aCGGATGTTGTTCACTTCCGATTCGAAGCTAATCAGCACATCGCCCAGGCCGCGCACGGCAAAAGTGGTGGACGCGCCACGACCGCCAGTATCGAACACATCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGttt                                                                                                                >  2:463106/1‑140 (MQ=255)

                   ggATGTTGTTCACTTCCGATTCGAAGCTAATCAGCACATCGCCCAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTg                                                                                                              >  1:26169/1‑140 (MQ=255)

                        ttgttCACTTCCGATTCGAAGCTAATCAGCACATCGCCCAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCttt                                                                                                         >  2:247093/1‑140 (MQ=255)

                        ttgttCACTTCCGATTCGAAGCTAATCAGCACATCGCCCAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCttt                                                                                                         >  1:129281/1‑140 (MQ=255)

                        ttgttCACTTCCGATTCGAAGCTAATCAGCACATCGCCCAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCttt                                                                                                         >  1:1035049/1‑140 (MQ=255)

                        ttgttCACTTCCGATTCGAAGCTAATCAGCACATCGCCCAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCttt                                                                                                         >  1:385830/1‑140 (MQ=255)

                        ttgttCACTTCCGATTCGAAGCTAATCAGCACATCGCCCAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCttt                                                                                                         >  2:834430/1‑140 (MQ=255)

                           ttCACTTCCGATTCGAAGCTAATCAGCACATCGCCCAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTc                                                                                                      >  1:749609/1‑140 (MQ=255)

                                ttCCGATTCGAAGCTAATCAGCACATCGCCCAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTcaccac                                                                                                 >  1:121713/1‑140 (MQ=255)

                                     ttccGAACCTAACCACCCCACCCCCCGGCCCGCCCCCGGAAAATGTGGTGTCCCCCCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCa                                                                                            <  2:305892/137‑1 (MQ=255)

                                                  cAGCCCTTCGCCCAGGCCGCGCTCGGAAAAAGTGGTGGTCGCGCCACGGCCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTg                                                                               <  1:387152/140‑1 (MQ=255)

                                                          cGCCCAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCAt                                                                       >  1:631239/1‑140 (MQ=255)

                                                          cGCCCAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCAt                                                                       >  2:86544/1‑140 (MQ=255)

                                                          cGCCCAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCAt                                                                       >  1:221922/1‑140 (MQ=255)

                                                          cGCCCAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCAt                                                                       >  2:157152/1‑140 (MQ=255)

                                                          cGCCCAGGCCGCGCTCGGCAAAAGTGGTGGCCGCCCCACGACCGCCAGTATCGAACACTTCACCGTTTTTCAGGGACTGGGCCATAAACGGTTCGGTTTTGCTTTTTCCACCCCCGTCAGCTTTATCCGCTGCCCCCCag                                                                       >  2:846173/1‑139 (MQ=255)

                                                             ccAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATgcc                                                                    <  1:834430/140‑1 (MQ=255)

                                                                 gCCGCGCTCGGCAAAGGTGGTGGCCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCa                                                                <  1:270904/140‑1 (MQ=255)

                                                                 gCCGCGCTCGGAAAAGGGGGGGGTCGCCCCCCGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCa                                                                <  2:340174/140‑1 (MQ=255)

                                                                   cgcgCTCGGCAAATGTGGGGGCCGCCCCGCTCCCGGCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGa                                                              <  2:93061/140‑1 (MQ=255)

                                                                     cgcTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATa                                                            >  1:94140/1‑140 (MQ=255)

                                                                           gCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGtata                                                      >  1:801054/1‑140 (MQ=255)

                                                                                  gggtggtCGCCCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATAAcgcgcg                                               <  1:616975/139‑1 (MQ=255)

                                                                                    gtggtCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATTACGCGCGtt                                             >  2:994044/1‑140 (MQ=255)

                                                                                        ccgccCCAGGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATAACGCGCGTTAccc                                         <  2:121713/139‑1 (MQ=255)

                                                                                                cGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATAACGCGCGTTACCCGACGtttt                                 >  1:305463/1‑140 (MQ=255)

                                                                                                     gCCAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATAACGCGCGTTACCCGACGTTttcgggt                            <  2:631239/140‑1 (MQ=255)

                                                                                                      ccAGTATCGAACACTTCAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATAACGCGCGTTACCCGACGTTttcgggtt                           >  1:763325/1‑140 (MQ=255)

                                                                                                      ccAGTATCGAACACTTCAACGATTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATAACGCGCGTTACCCGACGTTttcgggtt                           >  2:825812/1‑140 (MQ=255)

                                                                                                                      cAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATAACGCGCGTTACCCGACGTTTTCGGGTTCGGGAAAATCAGCTTc           >  1:833454/1‑140 (MQ=255)

                                                                                                                      cAACGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATAACGCGCGTTACCCGACGTTTTCGGGTTCGGGAAAATCAGCTTc           >  1:350240/1‑140 (MQ=255)

                                                                                                                         cGTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATAACGCGCGTTACCCGACGTTTTCGGGTTCGGGAAAATCAGCTTCACg        <  1:185600/140‑1 (MQ=255)

                                                                                                                          gTTTTTCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATAACGCGCGTTACCCGACGTTTTCGGGTTCGGGAAAATCAGCTTCACGt       <  1:247093/140‑1 (MQ=255)

                                                                                                                            ttttCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATAACGCGCGTTACCCGACGTTTTCGGGTTCGGGAAAATCAGCTTCGCGTCg     >  2:298285/1‑140 (MQ=255)

                                                                                                                              ttCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATAACGCGCGTTACCCGACGTTTTCGGGTTCGGGAAAATCAGCTTCACGTCGGa   >  2:683100/1‑140 (MQ=255)

                                                                                                                              ttCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATAACGCGCGTTACCCGACGTTTTCGGGTTCGGGAAAATCAGCTTCACGTCGGa   >  1:698684/1‑140 (MQ=255)

                                                                                                                              ttCAGGGACTGGGTCATAAACTGTTCGGTTTTGCC‑TTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATAACGCGCGTTACCCGACGTTTTCGGGTTCGGGAAAATCAGCTTCACGTCGGAg  <  1:858495/140‑1 (MQ=255)

                                                                                                                               tCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATAACGCGCGTTACCCGACGTTTTCGGGTTCGGGAAAATCAGCTTCACGTCGGAg  <  2:104657/140‑1 (MQ=255)

                                                                                                                               tCAGGGACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATAACGCGCGTTACCCGACGTTTTCGGGTTCGGGAAAATCAGCTTCACGTCGGAg  <  2:125423/140‑1 (MQ=255)

TGCGCTTCATACTGTTTACGGATGTTGTTCACTTCCGATTCGAAGCTAATCAGCACATCGCCCAGGCCGCGCTCGGCAAAAGTGGTGGTCGCGCCACGACCGCCAGTATCGAACACTTCAACGTTTTTCAGGAACTGGGTCATAAACTGTTCGGTTTTGCCTTTGTCACCACCGTCAGCTTTATCCGCTGCGCCCCATGCCGCCAGATAGGTATAACGCGCGTTACCCGACGTTTTCGGGTTCGGGAAAATCAGCTTCACGTCGGAG  >  NC_000913/2542837‑2543103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A