Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,789,783	G→A	E267K (GAA→AAA)	csiD →	PF08943 family protein CsiD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,789,783	0	G	A	100.0%	152.8 / NA	45	E267K (GAA→AAA)	csiD	PF08943 family protein CsiD
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (29/16); total (29/16)

AGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTGAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTGCACGGTCGCGACCGCTTTACTCCGCACCCGGATCTGCGCCGTGAACTGATGCGCCAGCG  >  NC_000913/2789651‑2789916

aGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAg                                                                                                                                >  1:407037/1‑140 (MQ=255)

    aaGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCaaa                                                                                                                            <  1:590157/140‑1 (MQ=255)

    aaGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCaaa                                                                                                                            <  1:778642/140‑1 (MQ=255)

      gATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAgg                                                                                                                          <  2:523815/140‑1 (MQ=255)

      gATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAgg                                                                                                                          <  2:104342/140‑1 (MQ=255)

       aTGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGc                                                                                                                         >  2:211878/1‑140 (MQ=255)

        tGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCa                                                                                                                        <  1:56031/140‑1 (MQ=255)

          tttttCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCAtt                                                                                                                      <  2:9816/140‑1 (MQ=255)

            tttCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCAttct                                                                                                                    >  2:741848/1‑140 (MQ=255)

               cATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCAttctttc                                                                                                                 >  2:576485/1‑140 (MQ=255)

                  ccGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCtgt                                                                                                              >  2:522186/1‑140 (MQ=255)

                  ccGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCtgg                                                                                                              >  2:449205/1‑139 (MQ=255)

                        ttCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGcccgt                                                                                                        >  2:831476/1‑140 (MQ=255)

                          cGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGcccgttc                                                                                                      >  2:409018/1‑140 (MQ=255)

                              gTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGcccgttcccgt                                                                                                  <  1:222667/140‑1 (MQ=255)

                                cGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTg                                                                                                <  2:991626/140‑1 (MQ=255)

                                    cAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCaa                                                                                            <  1:170209/140‑1 (MQ=255)

                                             cGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGtt                                                                                   >  2:503775/1‑140 (MQ=255)

                                               cccGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAGGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGa                                                                                 >  1:71114/1‑140 (MQ=255)

                                               cccGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGa                                                                                 >  1:550722/1‑140 (MQ=255)

                                               cccGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGa                                                                                 >  2:144744/1‑140 (MQ=255)

                                                              tatCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCt                                                                  >  2:63132/1‑140 (MQ=255)

                                                              tatCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCt                                                                  >  1:526927/1‑140 (MQ=255)

                                                              tatCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGGGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCt                                                                  >  1:59926/1‑140 (MQ=255)

                                                                   aCCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGCCGCCATTAAAACCAGCAAAGGCATTCTTTCTTTGCCCGTTCCCGTTGGCAAAATCCTGTTGATTAACAACCTGTTCTGGCTg                                                             >  1:687658/1‑140 (MQ=255)

                                                                   aCCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTg                                                             >  1:300760/1‑140 (MQ=255)

                                                                   aCCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTg                                                             >  1:380307/1‑140 (MQ=255)

                                                                   aCCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTg                                                             >  1:27716/1‑140 (MQ=255)

                                                                   aCCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTg                                                             >  1:80430/1‑140 (MQ=255)

                                                                   aCCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTg                                                             >  1:157686/1‑140 (MQ=255)

                                                                   aCCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTg                                                             >  2:887579/1‑140 (MQ=255)

                                                                        ttCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTGCACgg                                                        >  1:836871/1‑140 (MQ=255)

                                                                        ttCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTGCACgg                                                        >  2:478214/1‑140 (MQ=255)

                                                                        ttCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTGCACgg                                                        >  1:670505/1‑140 (MQ=255)

                                                                          cGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTGCACGGTc                                                      >  1:1023453/1‑140 (MQ=255)

                                                                                         cTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTGCACGGTCGCGACCGCTTTACTc                                       <  1:741848/140‑1 (MQ=255)

                                                                                               agaagGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTGCACGGTCGCGACCGCTTTACTCCGCAcc                                 <  1:503775/140‑1 (MQ=255)

                                                                                               agaagGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTGCACGGTCGCGACCGCTTTACTCCGCAcc                                 <  1:144744/140‑1 (MQ=255)

                                                                                                  agGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTGCACGGTCGCGACCGCTTTACTCCGCACCCgg                              >  2:722612/1‑140 (MQ=255)

                                                                                                         tgGTTGAGCGAGCTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTGCACGGTCGCGACCGCTTTACTCCGCACCCGGATCTgcg                       <  1:63132/140‑1 (MQ=255)

                                                                                                                  gagcTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTGCACGGTCGCGACCGCTTTACTCCGCACCCGGATCTGCGCCGTGAACt              >  1:346125/1‑140 (MQ=255)

                                                                                                                   agcTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTGCACGGTCGCGACCGCTTTACTCCGCACCCGGATCTGCGCCGTGAACTg             <  1:887579/140‑1 (MQ=255)

                                                                                                                   agcTTTCCGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTGCACGGTCGCGACCGCTTTACTCCGCACCCGGATCTGCGCCGTGAACTg             >  1:671161/1‑140 (MQ=255)

                                                                                                                          cGACGCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTGCACGGTCGCGACCGCTTTACTCCGCACCCGGATCTGCGCCGTGAACTGATGCGcc      <  2:380307/140‑1 (MQ=255)

                                                                                                                              gCCATTAAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTGCACGGTCGCGACCGCTTTACTCCGCACCCGGATCTGCGCCGTGAACTGATGCGCCAGCg  <  2:670505/140‑1 (MQ=255)

AGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTCGTCCAGCCGAAAGACTTCGAAGAAGGCGTGTGGTTGAGCGAGCTTTCCGACGCCATTGAAACCAGCAAAGGCATTCTTTCTGTGCCCGTTCCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTGCACGGTCGCGACCGCTTTACTCCGCACCCGGATCTGCGCCGTGAACTGATGCGCCAGCG  >  NC_000913/2789651‑2789916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A