Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	120	97.0	3206026	99.3%	3183583	140.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	527,577	T→C	intergenic (+36/‑4)	rhsD → / → ybbC	protein RhsD/PF15631 family protein YbbC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	527,577	0	T	C	100.0%	143.4 / NA	38	intergenic (+36/‑4)	rhsD/ybbC	protein RhsD/PF15631 family protein YbbC
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/18); total (20/18)

CCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGATTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACT  >  NC_000913/527440‑527713

ccTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTc                                                                                                                                        >  1:1349633/1‑140 (MQ=255)

     tGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGa                                                                                                                                   >  1:793353/1‑140 (MQ=255)

         cGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAata                                                                                                                               <  1:76310/140‑1 (MQ=255)

         cGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAata                                                                                                                               >  1:1568024/1‑140 (MQ=255)

           aTTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAAtata                                                                                                                             >  1:577877/1‑140 (MQ=255)

           aTTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAAtata                                                                                                                             >  2:1110273/1‑140 (MQ=255)

                     cTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATAtt                                                                                                                   >  2:457158/1‑140 (MQ=255)

                      ttAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATAttt                                                                                                                  >  1:1266679/1‑140 (MQ=255)

                                aGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCttt                                                                                                        >  1:960178/1‑140 (MQ=255)

                                aGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCttt                                                                                                        >  2:770538/1‑140 (MQ=255)

                                         tatTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTta                                                                                               <  2:197341/140‑1 (MQ=255)

                                          atTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTtat                                                                                              <  2:1319893/140‑1 (MQ=255)

                                               tATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTAt                                                                                         >  1:978027/1‑140 (MQ=255)

                                               tATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTAt                                                                                         <  1:162121/140‑1 (MQ=255)

                                               tATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTAt                                                                                         >  1:104911/1‑140 (MQ=255)

                                                aTCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTAtt                                                                                        <  2:1266679/140‑1 (MQ=255)

                                                aTCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTAtt                                                                                        <  2:793353/140‑1 (MQ=255)

                                                  cAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATGTAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTg                                                                                      <  1:1110273/140‑1 (MQ=255)

                                                    gAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGcc                                                                                    <  1:1095560/140‑1 (MQ=255)

                                                            gtCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATga                                                                            <  1:1518422/140‑1 (MQ=255)

                                                               ccGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGAc                                                                         >  1:1557927/1‑140 (MQ=255)

                                                                cGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACa                                                                        <  1:1407741/140‑1 (MQ=255)

                                                                cGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACa                                                                        <  2:444688/140‑1 (MQ=255)

                                                                           tCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACgg                                                             <  2:1349633/140‑1 (MQ=255)

                                                                            cAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTC‑ATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGtt                                                           <  2:552626/140‑1 (MQ=255)

                                                                                       ccGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGaaaa                                                 >  2:1207241/1‑140 (MQ=255)

                                                                                            tCATGATTAATTTTAGTGCTTTTATTAGAGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACatta                                            >  2:206318/1‑140 (MQ=255)

                                                                                                  ttAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTa                                      >  2:1539180/1‑140 (MQ=255)

                                                                                                   tAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTAt                                     <  2:163575/140‑1 (MQ=255)

                                                                                                    aaTTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATg                                    <  1:297528/140‑1 (MQ=255)

                                                                                                                         ggggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAt               >  1:975967/1‑140 (MQ=255)

                                                                                                                          gggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              <  2:1568024/140‑1 (MQ=255)

                                                                                                                          gggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              >  1:258084/1‑140 (MQ=255)

                                                                                                                           ggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAttt             >  2:494125/1‑140 (MQ=255)

                                                                                                                             ccTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAg           >  1:120624/1‑140 (MQ=255)

                                                                                                                               tataAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGAt         <  2:1557927/140‑1 (MQ=255)

                                                                                                                                 taAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATaa       >  1:1593756/1‑140 (MQ=255)

                                                                                                                                      agaCTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAct  <  2:960178/140‑1 (MQ=255)

CCTGATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGATTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACT  >  NC_000913/527440‑527713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A