Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	120	97.0	3206026	99.3%	3183583	140.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,580,434	(G)_5→4	coding (357/1158 nt)	ydeM ←	putative anaerobic sulfatase maturation enzyme YdeM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,580,430	0	G	.	98.7%	341.1 / ‑2.2	77	coding (361/1158 nt)	ydeM	putative anaerobic sulfatase maturation enzyme YdeM
Reads supporting (aligned to +/- strand): ref base G (1/0); new base . (35/41); total (36/41)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

TAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGAT  >  NC_000913/1580291‑1580561

tAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTg                                                                                                                                     >  1:1035017/1‑140 (MQ=255)

tAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTg                                                                                                                                     >  2:856030/1‑140 (MQ=255)

tAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTg                                                                                                                                     >  1:1461863/1‑140 (MQ=255)

      aCATTAACGTTATTAATGACGGGTAACGTATTAAACTCTACTTGATATGATTGCAGGCGCTCGAGGGCTGCTATCTCTTTGGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTggggcca                                                                                                                               <  2:1223988/140‑4 (MQ=255)

        aTTAACGTTATTAATGACGGTTAACGTATTAAACTCTGCTTGATATGATTTCAGACGCTCGATGGCTGCTTTCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCatc                                                                                                                            <  1:1171403/140‑1 (MQ=255)

        aTTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCatc                                                                                                                            <  2:1416070/140‑1 (MQ=255)

        aTTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCatc                                                                                                                            <  2:556709/140‑1 (MQ=255)

        aTTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCatc                                                                                                                            >  1:260016/1‑140 (MQ=255)

        aTTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCatc                                                                                                                            <  2:75766/140‑1 (MQ=255)

        aTTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCatc                                                                                                                            >  1:1397194/1‑140 (MQ=255)

          tAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCatcga                                                                                                                          >  2:1182520/1‑140 (MQ=255)

          tAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCatcga                                                                                                                          >  2:747804/1‑140 (MQ=255)

          tAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCatcga                                                                                                                          <  2:902459/140‑1 (MQ=255)

           aaCGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCatcgat                                                                                                                         <  1:391080/140‑1 (MQ=255)

                 attaATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGAt                                                                                                                   >  1:563170/1‑140 (MQ=255)

                 attaATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGAt                                                                                                                   >  1:1070034/1‑140 (MQ=255)

                  ttaATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATa                                                                                                                  >  2:805749/1‑140 (MQ=255)

                    aaTGACGGTTAACGTATTAATCTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATacc                                                                                                                <  1:1228438/140‑1 (MQ=255)

                      tGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATaccaa                                                                                                              >  2:1522419/1‑140 (MQ=255)

                        aCGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATaccaacc                                                                                                            >  1:110144/1‑140 (MQ=255)

                        aCGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGCTCATGTAACTCCTGGGGGCCATCGATCGAGATaccaac                                                                                                             >  2:1536714/1‑140 (MQ=255)

                            ttAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGaa                                                                                                        >  1:1035269/1‑140 (MQ=255)

                            ttAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGaa                                                                                                        >  2:1023954/1‑140 (MQ=255)

                            ttAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGaa                                                                                                        >  2:790121/1‑140 (MQ=255)

                               aCGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAAtt                                                                                                     >  1:287243/1‑140 (MQ=255)

                               aCGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAAtt                                                                                                     >  1:52202/1‑140 (MQ=255)

                                   aTTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATg                                                                                                 <  2:1461863/140‑1 (MQ=255)

                                      aaaCTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTc                                                                                              <  1:1323128/140‑1 (MQ=255)

                                       aaCTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCt                                                                                             <  2:1282433/140‑1 (MQ=255)

                                           ctACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTga                                                                                         >  2:1526955/1‑140 (MQ=255)

                                            tACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTgag                                                                                        >  1:881368/1‑140 (MQ=255)

                                            tACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTgag                                                                                        <  1:1512595/140‑1 (MQ=255)

                                            tACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTgag                                                                                        >  2:1568535/1‑140 (MQ=255)

                                            tACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTgag                                                                                        >  2:192831/1‑140 (MQ=255)

                                            tACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTgag                                                                                        >  2:852007/1‑140 (MQ=255)

                                              cTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGaa                                                                                      >  2:1221087/1‑140 (MQ=255)

                                                   tatGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGca                                                                                 <  2:1029300/140‑1 (MQ=255)

                                                     tGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGcaca                                                                               <  1:747804/140‑1 (MQ=255)

                                                     tGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGcaca                                                                               <  1:856030/140‑1 (MQ=255)

                                                     tGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGcaca                                                                               <  1:576515/140‑1 (MQ=255)

                                                       aTTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACAcc                                                                             <  2:375483/140‑1 (MQ=255)

                                                       aTTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACAcc                                                                             <  2:1052592/140‑1 (MQ=255)

                                                          tCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACcatt                                                                          <  1:729719/140‑1 (MQ=255)

                                                           cAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACcattc                                                                         >  2:666079/1‑140 (MQ=255)

                                                           cAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACcattc                                                                         >  2:144592/1‑140 (MQ=255)

                                                            aGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACcattca                                                                        <  2:1035017/140‑1 (MQ=255)

                                                            aGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACcattca                                                                        <  1:1276396/140‑1 (MQ=255)

                                                            aGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACcattca                                                                        <  2:795981/140‑1 (MQ=255)

                                                            aGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACcattca                                                                        <  1:805749/140‑1 (MQ=255)

                                                             gACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACcattcat                                                                       >  2:384093/1‑140 (MQ=255)

                                                              aCGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACcattcatt                                                                      >  2:978692/1‑140 (MQ=255)

                                                                              aTCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCa                                                      <  2:623422/140‑1 (MQ=255)

                                                                              aTCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCa                                                      <  1:1023954/140‑1 (MQ=255)

                                                                              aTCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCa                                                      <  2:17447/140‑1 (MQ=255)

                                                                              aTCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCa                                                      <  2:287243/140‑1 (MQ=255)

                                                                                    tttGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGtt                                                >  1:542407/1‑140 (MQ=255)

                                                                                     ttGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGttt                                               >  1:552757/1‑140 (MQ=255)

                                                                                      tGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTg                                              >  1:714048/1‑140 (MQ=255)

                                                                                        cAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTa                                            <  2:1070034/140‑1 (MQ=255)

                                                                                        cAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTa                                            <  1:934235/140‑1 (MQ=255)

                                                                                         aaaaGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTaa                                           <  2:563170/140‑1 (MQ=255)

                                                                                           aaGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATg                                         <  1:1526955/140‑1 (MQ=255)

                                                                                                      ccTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATa                              <  1:192831/140‑1 (MQ=255)

                                                                                                        tGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACg                            <  1:790121/140‑1 (MQ=255)

                                                                                                           aTTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGttt                         <  2:52202/140‑1 (MQ=255)

                                                                                                           aTTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGttt                         <  1:53579/140‑1 (MQ=255)

                                                                                                           aTTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGttt                         <  2:850065/140‑1 (MQ=255)

                                                                                                                tGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACTAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAGAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGc                    <  1:161165/140‑1 (MQ=255)

                                                                                                                tGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGc                    <  2:277435/140‑1 (MQ=255)

                                                                                                                tGCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGc                    >  2:868835/1‑140 (MQ=255)

                                                                                                                 gCGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGcc                   >  2:667730/1‑140 (MQ=255)

                                                                                                                  cGTCTGTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCt                  >  2:1320815/1‑140 (MQ=255)

                                                                                                                       gTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATa             <  2:714048/140‑1 (MQ=255)

                                                                                                                       gTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATa             <  1:1221087/140‑1 (MQ=255)

                                                                                                                       gTAACGGTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATa             <  2:880203/140‑1 (MQ=255)

                                                                                                                            ggTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgtt        >  2:1111176/1‑140 (MQ=255)

                                                                                                                             gTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgttg       >  1:447491/1‑140 (MQ=255)

                                                                                                                             gTCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgttg       >  1:1038530/1‑140 (MQ=255)

                                                                                                                              tCATGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgttgt      >  1:587004/1‑140 (MQ=255)

                                                                                                                                 tGTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGa   <  1:852007/140‑1 (MQ=255)

                                                                                                                                  gTAACTCCT‑GGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGAt  >  2:832549/1‑140 (MQ=255)

TAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGAT  >  NC_000913/1580291‑1580561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A