Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	120	97.0	3206026	99.3%	3183583	140.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,959,539	(T)_5→4	coding (308/567 nt)	yecM ←	putative metal‑binding enzyme YecM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,959,535	0	T	.	94.7%	377.2 / 16.2	95	coding (312/567 nt)	yecM	putative metal‑binding enzyme YecM
Reads supporting (aligned to +/- strand): ref base T (3/2); new base . (42/48); total (45/50)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 105 total aligned reads displayed.

TTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAA  >  NC_000913/1959396‑1959668

ttGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGt                                                                                                                                       >  2:402569/1‑140 (MQ=255)

 tGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGtt                                                                                                                                      >  2:267697/1‑140 (MQ=255)

     gAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGttttcc                                                                                                                                  >  1:739597/1‑138 (MQ=255)

      aaCTGGTTTTCACTGAGATCCCCGGTGGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTccc                                                                                                                                 <  2:764511/140‑1 (MQ=255)

      aaCTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGttttccc                                                                                                                                 <  2:1046361/140‑4 (MQ=255)

      aaCTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGttttccc                                                                                                                                 <  2:915095/140‑4 (MQ=255)

      aaCTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGttttccc                                                                                                                                 <  1:964867/140‑4 (MQ=255)

      aaCTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGttttccc                                                                                                                                 <  1:492925/140‑4 (MQ=255)

      aaCTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGttttccc                                                                                                                                 <  1:1164009/140‑4 (MQ=255)

      aaCTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGttttccc                                                                                                                                 <  2:617135/140‑4 (MQ=255)

      aaCTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGttttccc                                                                                                                                 <  2:250435/140‑4 (MQ=255)

       aCTGGTTTTCACTGAGCTCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTccc                                                                                                                                <  1:843746/140‑1 (MQ=255)

       aCTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTcccc                                                                                                                                >  2:276804/1‑140 (MQ=255)

       aCTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTcccc                                                                                                                                >  2:473699/1‑140 (MQ=255)

           gTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGc                                                                                                                           <  1:94057/140‑1 (MQ=255)

           gTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGc                                                                                                                           <  1:337192/140‑1 (MQ=255)

              ttCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCAc                                                                                                                        <  1:1501919/140‑1 (MQ=255)

              ttCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCAc                                                                                                                        <  2:336259/140‑1 (MQ=255)

                 aCTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGt                                                                                                                     <  1:1420931/140‑1 (MQ=255)

                   tGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAg                                                                                                                   <  1:163483/140‑1 (MQ=255)

                   tGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAg                                                                                                                   <  1:24329/140‑1 (MQ=255)

                   tGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAg                                                                                                                   <  2:728061/140‑1 (MQ=255)

                     agaTCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCt                                                                                                                 >  2:589462/1‑140 (MQ=255)

                     agaTCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCt                                                                                                                 >  1:1218272/1‑140 (MQ=255)

                      gaTCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTc                                                                                                                <  1:1308606/140‑1 (MQ=255)

                      gaTCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTc                                                                                                                <  1:200942/140‑1 (MQ=255)

                          cccGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCcaca                                                                                                            >  2:123938/1‑140 (MQ=255)

                          cccGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCcaca                                                                                                            >  2:578099/1‑140 (MQ=255)

                           ccGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACaa                                                                                                           >  2:28137/1‑140 (MQ=255)

                                 ggCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGaaa                                                                                                     >  1:23089/1‑140 (MQ=255)

                                    tGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGaaaccc                                                                                                  >  2:706496/1‑137 (MQ=255)

                                    tGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACt                                                                                                  >  2:918134/1‑140 (MQ=255)

                                      aGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGc                                                                                                >  2:1079533/1‑140 (MQ=255)

                                      aGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGc                                                                                                <  1:1000895/140‑1 (MQ=255)

                                      aGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGc                                                                                                <  2:516190/140‑1 (MQ=255)

                                      aGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGc                                                                                                >  2:51013/1‑140 (MQ=255)

                                       gTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGcc                                                                                               >  2:342730/1‑140 (MQ=255)

                                       gTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGcc                                                                                               <  1:1559229/140‑1 (MQ=255)

                                        tCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCa                                                                                              >  2:604407/1‑140 (MQ=255)

                                        tCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCa                                                                                              <  2:1090265/140‑1 (MQ=255)

                                         ccTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATGGAAAACTGCCAg                                                                                             >  1:376503/1‑140 (MQ=255)

                                             catcaGAAAGCAATGCCAGCGCGCGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTgcg                                                                                         <  2:1437714/140‑1 (MQ=255)

                                             catcaGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTgcg                                                                                         <  2:1581027/140‑1 (MQ=255)

                                             catcaGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTgcg                                                                                         <  2:1548193/140‑1 (MQ=255)

                                             catcaGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTgcg                                                                                         <  2:1508154/140‑1 (MQ=255)

                                             catcaGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTgcg                                                                                         <  2:1472449/140‑1 (MQ=255)

                                             catcaGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTgcg                                                                                         <  1:520023/140‑1 (MQ=255)

                                             catcaGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTgcg                                                                                         <  1:123938/140‑1 (MQ=255)

                                             catcaGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTgcg                                                                                         <  2:1019708/140‑1 (MQ=255)

                                             catcaGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTCCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTgcg                                                                                         <  2:881404/140‑1 (MQ=255)

                                              atcaGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTgcgc                                                                                        <  1:752536/140‑1 (MQ=255)

                                                caggaaGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTT‑‑CCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAAc                                                                                     >  2:949136/5‑140 (MQ=255)

                                                 aGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAAc                                                                                     <  1:1012508/140‑1 (MQ=255)

                                                 aGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAAc                                                                                     <  1:765634/140‑1 (MQ=255)

                                                 aGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAAc                                                                                     <  1:1180893/140‑1 (MQ=255)

                                                     aGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGt                                                                                 >  2:285512/1‑140 (MQ=255)

                                                              aGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCa                                                                        <  1:377179/140‑1 (MQ=255)

                                                               gcgcACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCAt                                                                       >  1:537759/1‑140 (MQ=255)

                                                               gcgcACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCAt                                                                       <  1:1112752/140‑1 (MQ=255)

                                                               gcgcACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCAt                                                                       <  2:1437648/140‑1 (MQ=255)

                                                                    cGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAAt                                                                  >  2:1209444/1‑140 (MQ=255)

                                                                    cGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAAt                                                                  >  2:241891/1‑140 (MQ=255)

                                                                     gggCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAAtt                                                                 >  1:124709/1‑140 (MQ=255)

                                                                      ggCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAAttt                                                                >  1:1101525/1‑140 (MQ=255)

                                                                       gCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTa                                                               <  2:174415/140‑1 (MQ=255)

                                                                          ttCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAAc                                                            >  2:174951/1‑140 (MQ=255)

                                                                           tCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACa                                                           <  1:1205076/140‑1 (MQ=255)

                                                                                gTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACaa                                                      <  1:1259859/140‑1 (MQ=255)

                                                                                 tttCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACaaa                                                     >  1:449955/1‑140 (MQ=255)

                                                                                    cTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCg                                                  <  2:799314/140‑1 (MQ=255)

                                                                                     tGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCgg                                                 >  1:1501174/1‑140 (MQ=255)

                                                                                      ggATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGt                                                <  2:310864/140‑1 (MQ=255)

                                                                                      ggATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGt                                                <  1:1082435/140‑1 (MQ=255)

                                                                                      ggATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGt                                                <  2:1101525/140‑1 (MQ=255)

                                                                                      ggATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGt                                                <  1:578099/140‑1 (MQ=255)

                                                                                       gATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTc                                               >  1:880780/1‑140 (MQ=255)

                                                                                         tCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTg                                             <  1:28137/140‑1 (MQ=255)

                                                                                           aCCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGcc                                           >  2:470240/1‑140 (MQ=255)

                                                                                            cccGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCa                                          >  1:460492/1‑140 (MQ=255)

                                                                                            cccGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCa                                          >  2:1122349/1‑140 (MQ=255)

                                                                                            cccGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCa                                          >  2:491534/1‑140 (MQ=255)

                                                                                            cccGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCa                                          >  1:572100/1‑140 (MQ=255)

                                                                                                    aaCGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCat                                  >  1:1155078/1‑140 (MQ=255)

                                                                                                              atatGTTCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGAc                        <  1:604407/140‑1 (MQ=255)

                                                                                                                    tCCCAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGc                  >  1:561768/1‑140 (MQ=255)

                                                                                                                       cAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCg               <  1:446462/140‑1 (MQ=255)

                                                                                                                       cAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCg               <  2:1218272/140‑1 (MQ=255)

                                                                                                                       cAACCTTCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCg               >  2:1514703/1‑140 (MQ=255)

                                                                                                                            ttCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCATCCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCcaca          <  1:323854/140‑1 (MQ=255)

                                                                                                                            ttCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAGCCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCcaca          <  1:276804/140‑1 (MQ=255)

                                                                                                                            ttCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCcaca          <  2:818430/140‑1 (MQ=255)

                                                                                                                            ttCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCcaca          <  1:1079533/140‑1 (MQ=255)

                                                                                                                             tCGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCacac         >  2:205819/1‑140 (MQ=255)

                                                                                                                             tCGTGTGGGTAACG‑GTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCacac         >  1:633207/1‑140 (MQ=255)

                                                                                                                              cGTGTGGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACt        >  1:1297262/1‑140 (MQ=255)

                                                                                                                                tgtgGGTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGt      >  2:484835/1‑140 (MQ=255)

                                                                                                                                    ggTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTcga  >  2:128880/1‑138 (MQ=255)

                                                                                                                                    ggTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCaa  >  1:1344441/1‑140 (MQ=255)

                                                                                                                                    ggTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCaa  >  2:482989/1‑140 (MQ=255)

                                                                                                                                    ggTAACG‑TTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCaa  >  2:1363425/1‑140 (MQ=255)

TTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAA  >  NC_000913/1959396‑1959668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A