Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	134	60.5	2175152	94.0%	2044642	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,723,923	A→G	P399P (CCA→CCG)	pssA →	phosphatidylserine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,723,923	0	A	G	98.7%	285.4 / ‑4.5	79	P399P (CCA→CCG)	pssA	phosphatidylserine synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (37/41); minor base T (1/0); total (38/41)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

CACCTATCACCTGAAAGGGATGTGGGTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACC  >  NC_000913/2723788‑2724059

cacCTATCACCTGAAAGGGATGTGGGTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGc                                                                                                                                      >  2:861413/1‑140 (MQ=255)

cacCTATCACCTGAAAGGGATGTGGGTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGc                                                                                                                                      >  1:439382/1‑140 (MQ=255)

 acCTATCACCTGAAAGGGATGTGGGTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCg                                                                                                                                     <  1:730509/140‑1 (MQ=255)

  ccTATCACCTGAAAGGGATGTGGGTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCga                                                                                                                                    <  2:480128/140‑1 (MQ=255)

      tCACCTGAAAGGGATGTGGGTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCgagaga                                                                                                                                <  2:129464/140‑1 (MQ=255)

           tGAAAGGGATGTGGGTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGagaaagaa                                                                                                                           >  1:834432/1‑140 (MQ=255)

             aaaGGGATGTGGGTTGATGATAAGTGGATGTTGATCACCGGTAATGACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCCCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACt                                                                                                                         >  2:746741/1‑140 (MQ=255)

             aaaGGGATGTGGGTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGagaa                                                                                                                               >  1:664951/1‑134 (MQ=255)

             aaaGGGATGTGGGTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGagaa                                                                                                                               <  2:664951/134‑1 (MQ=255)

             aaaGGGATGTGGGTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACt                                                                                                                         >  2:170369/1‑140 (MQ=255)

             aaaGGGATGTGGGTTGATGATAAGTGGATGTTGATCACC‑GTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTg                                                                                                                        >  2:752087/1‑140 (MQ=255)

                     gtgGGTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGa                                                                                                                 <  2:439382/140‑1 (MQ=255)

                         gTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCcg                                                                                                             <  1:170369/140‑1 (MQ=255)

                          ttGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCcgc                                                                                                            <  2:414395/140‑1 (MQ=255)

                           tgatgaTAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCcgcg                                                                                                           >  1:790623/1‑140 (MQ=255)

                           tgatgaTAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGAACTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCcgcg                                                                                                           <  2:139101/140‑1 (MQ=255)

                            gatgatAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGa                                                                                                          >  2:643219/1‑140 (MQ=255)

                            gatgatAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGa                                                                                                          >  1:699719/1‑140 (MQ=255)

                            gatgatAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGa                                                                                                          >  1:808803/1‑140 (MQ=255)

                            gatgatAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGa                                                                                                          >  2:871915/1‑140 (MQ=255)

                            gatgatAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGa                                                                                                          >  1:296765/1‑140 (MQ=255)

                            gatgatAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGa                                                                                                          >  2:510626/1‑140 (MQ=255)

                             atgatAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAg                                                                                                         <  2:834432/140‑1 (MQ=255)

                             atgatAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAg                                                                                                         >  1:1060671/1‑140 (MQ=255)

                                  aaGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATac                                                                                                    <  1:935459/140‑1 (MQ=255)

                                  aaGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATac                                                                                                    >  1:481716/1‑140 (MQ=255)

                                    gTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATacca                                                                                                  <  1:641744/140‑1 (MQ=255)

                                     tGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATaccac                                                                                                 <  1:746741/140‑1 (MQ=255)

                                       gATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCcgc                                                                                                            <  1:494396/127‑1 (MQ=255)

                                       gATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCcgc                                                                                                            >  2:494396/1‑127 (MQ=255)

                                       gATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATAccacca                                                                                               <  2:865658/140‑1 (MQ=255)

                                       gATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATAccacca                                                                                               <  1:130105/140‑1 (MQ=255)

                                        aTGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCAt                                                                                              <  1:884551/140‑1 (MQ=255)

                                           ttGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGt                                                                                           >  1:90216/1‑140 (MQ=255)

                                            tGATCACC‑GTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTa                                                                                         <  1:752087/140‑1 (MQ=255)

                                               tCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAg                                                                                       >  1:1018050/1‑140 (MQ=255)

                                               tCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAg                                                                                       >  1:766183/1‑140 (MQ=255)

                                               tCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAg                                                                                       >  2:179754/1‑140 (MQ=255)

                                               tCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAg                                                                                       <  1:519274/140‑1 (MQ=255)

                                               tCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAg                                                                                       >  2:175195/1‑140 (MQ=255)

                                                 aCCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCa                                                                                     <  2:790623/140‑1 (MQ=255)

                                                 aCCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCa                                                                                     <  2:296765/140‑1 (MQ=255)

                                                 aCCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCa                                                                                     <  2:699719/140‑1 (MQ=255)

                                                 aCCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCa                                                                                     <  1:643219/140‑1 (MQ=255)

                                                    ggTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCa                                                                                     <  2:602120/137‑1 (MQ=255)

                                                    ggTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCa                                                                                     >  1:602120/1‑137 (MQ=255)

                                                    ggTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTa                                                                                  <  1:877526/140‑1 (MQ=255)

                                                     gTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTAt                                                                                 >  1:199690/1‑140 (MQ=255)

                                                       aataaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATcg                                                                               <  2:408615/140‑1 (MQ=255)

                                                        ataaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATcgc                                                                              >  2:803208/1‑140 (MQ=255)

                                                        ataaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATcgc                                                                              >  1:1064570/1‑140 (MQ=255)

                                                         taaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATcgcg                                                                             <  2:1081984/140‑1 (MQ=255)

                                                         taaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATcgcg                                                                             <  1:617502/140‑1 (MQ=255)

                                                         taaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATcgcg                                                                             <  1:769581/140‑1 (MQ=255)

                                                         taaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATcgcg                                                                             <  1:179754/140‑1 (MQ=255)

                                                           aCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGAt                                                                           <  2:481716/140‑1 (MQ=255)

                                                              tGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTg                                                                        >  2:674461/1‑140 (MQ=255)

                                                                aaCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCGCGATCCGCAACTTGAGCTGGCGCCTCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCa                                                                      >  1:417338/1‑140 (MQ=255)

                                                                aaCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCa                                                                      >  2:481224/1‑140 (MQ=255)

                                                                  cccGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCaaa                                                                    <  2:199690/140‑1 (MQ=255)

                                                                      cgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTAt                                                                >  1:246931/1‑140 (MQ=255)

                                                                      cgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTAt                                                                >  2:736969/1‑140 (MQ=255)

                                                                       gcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTAtt                                                               <  2:10920/140‑1 (MQ=255)

                                                                         gcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGc                                                             <  1:871915/140‑1 (MQ=255)

                                                                            tGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGa                                                          <  1:674461/140‑1 (MQ=255)

                                                                               cGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTa                                                       <  1:175195/140‑1 (MQ=255)

                                                                                   tgtatctggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCg                                                   >  2:650927/4‑140 (MQ=255)

                                                                                         tggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAg                                             <  2:1018050/140‑1 (MQ=255)

                                                                                          ggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGGGAAga                                            <  2:808803/140‑2 (MQ=255)

                                                                                           gaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGt                                           >  2:538585/1‑140 (MQ=255)

                                                                                             aatcGCCATTTGGATCCGCGTTTTGCACATTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCCATTTGCAAAGTATTGCCGATTATCCGTTGACGGTTc                                         <  2:94731/137‑1 (MQ=255)

                                                                                                           tCCACGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATccgc                           >  1:125330/1‑140 (MQ=255)

                                                                                                               cGATCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGtt                       >  1:458913/1‑140 (MQ=255)

                                                                                                                 aTCCGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTg                     <  1:736969/140‑1 (MQ=255)

                                                                                                                    cGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTgcgc                  <  2:458913/140‑1 (MQ=255)

                                                                                                                    cGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTgcgc                  <  1:538585/140‑1 (MQ=255)

                                                                                                                    cGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTg                     >  2:415652/1‑137 (MQ=255)

                                                                                                                    cGCAACTTGAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTg                     <  1:415652/137‑1 (MQ=255)

                                                                                                                            gAGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCg          >  2:526273/1‑140 (MQ=255)

                                                                                                                             aGCTGGCGCCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGc         >  2:883575/1‑140 (MQ=255)

                                                                                                                                   cgccgcAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTgcgc                  >  2:256273/1‑125 (MQ=255)

                                                                                                                                   cgccgcAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTgcgc                  <  1:256273/125‑1 (MQ=255)

                                                                                                                                   cgccgcAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGAc   >  1:81593/1‑140 (MQ=255)

                                                                                                                                    gcCGCAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGAcc  <  1:510626/140‑1 (MQ=255)

CACCTATCACCTGAAAGGGATGTGGGTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACC  >  NC_000913/2723788‑2724059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A