Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	134	60.5	2175152	94.0%	2044642	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,951,007	G→A	A485A (GCC→GCT)	recD ←	exodeoxyribonuclease V subunit RecD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,951,007	0	G	A	100.0%	175.5 / NA	50	A485A (GCC→GCT)	recD	exodeoxyribonuclease V subunit RecD
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (30/20); total (30/20)

TCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCATTCAGTCCAGCCACGCCAAACGGCCCTTCCCGCAGGGCGCACA  >  NC_000913/2950870‑2951140

tCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGTCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGc                                                                                                                                     >  1:693110/1‑140 (MQ=255)

   tGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAat                                                                                                                                  <  1:866514/140‑1 (MQ=255)

   tGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAat                                                                                                                                  >  2:866514/1‑140 (MQ=255)

    gCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACTAGCaa                                                                                                                                   >  2:85180/1‑138 (MQ=255)

       ctGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAatcatc                                                                                                                              <  1:712152/140‑1 (MQ=255)

                  cTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGAcc                                                                                                                   >  2:884324/1‑140 (MQ=255)

                   tCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCt                                                                                                                  <  2:164376/140‑1 (MQ=255)

                    cGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCtt                                                                                                                 >  2:235023/1‑140 (MQ=255)

                     ggTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTc                                                                                                                <  1:884324/140‑1 (MQ=255)

                             aGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAAc                                                                                                        <  1:235023/140‑1 (MQ=255)

                                         gtcGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGAt                                                                                            >  1:723731/3‑140 (MQ=255)

                                         gccggccgGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGGCGACCTTCGTACCAACGAGAGTGCGGAt                                                                                            >  2:618434/1‑140 (MQ=255)

                                         gCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATGTCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGAt                                                                                            >  1:1001081/1‑140 (MQ=255)

                                         gCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTATGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGAt                                                                                            >  2:480890/1‑140 (MQ=255)

                                         gCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGac                                                                                            >  1:814045/1‑139 (MQ=255)

                                         gCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGAt                                                                                            >  2:208952/1‑140 (MQ=255)

                                         gCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGAt                                                                                            >  1:105754/1‑140 (MQ=255)

                                                       cAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTg                                                                              <  2:566952/140‑1 (MQ=255)

                                                              gACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTATGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCt                                                                       <  1:480890/140‑1 (MQ=255)

                                                              gACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCt                                                                       <  1:158129/140‑1 (MQ=255)

                                                              gACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCt                                                                       <  2:1001081/140‑1 (MQ=255)

                                                              gACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGACGACGATGAATTTTGCGCTTCt                                                                       <  2:814045/140‑1 (MQ=255)

                                                               aCGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTg                                                                      >  1:320432/1‑140 (MQ=255)

                                                                   gcgTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGc                                                                  <  2:1012290/140‑1 (MQ=255)

                                                                     gTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCAt                                                                >  2:1021193/1‑140 (MQ=255)

                                                                        cccTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATaaa                                                             <  1:1021193/140‑1 (MQ=255)

                                                                             cccGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCt                                                                       >  1:88135/1‑125 (MQ=255)

                                                                             cccGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCt                                                                       <  2:88135/125‑1 (MQ=255)

                                                                             cccGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCt                                                        >  1:373996/1‑140 (MQ=255)

                                                                               cgcgATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACTATGAATTTTGCGCTTCTGTTGCATAAACTGCTCa                                                      >  1:633093/1‑140 (MQ=255)

                                                                                 cgATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGc                                                         >  2:799404/1‑135 (MQ=255)

                                                                                 cgATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGc                                                         <  1:799404/135‑1 (MQ=255)

                                                                                     ccAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGc                                                <  2:373996/140‑1 (MQ=255)

                                                                                          gcAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCAtt                                           <  1:101445/140‑1 (MQ=255)

                                                                                          gcAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCAtt                                           <  2:723731/140‑1 (MQ=255)

                                                                                              tACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAGCTGCTCAATTCGCTCATTCAGt                                       >  1:765843/1‑140 (MQ=255)

                                                                                              tACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCGTTCAGt                                       >  1:654374/1‑140 (MQ=255)

                                                                                              tACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCATTCAGt                                       >  2:701682/1‑140 (MQ=255)

                                                                                              tACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCATTCAGt                                       >  2:71797/1‑140 (MQ=255)

                                                                                              tACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCATTCAGt                                       >  1:94527/1‑140 (MQ=255)

                                                                                              tACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCATTCAGt                                       >  1:271101/1‑140 (MQ=255)

                                                                                                      tCGCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCATTCAGTCCAGCCAc                               <  2:693110/140‑1 (MQ=255)

                                                                                                        gCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCATTCAGTCCAGCCACGc                             >  1:202267/1‑140 (MQ=255)

                                                                                                        gCCATTAAACAACCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCATTCAGTCCAGCCACGc                             >  1:19255/1‑140 (MQ=255)

                                                                                                                acaacCCAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCATTCAGTCCAGCCACGCCAAACGGc                     <  1:701682/140‑1 (MQ=255)

                                                                                                                    cccAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCATTCAGTCCAGCCACGCCAAACGGCCCtt                 >  1:618309/1‑140 (MQ=255)

                                                                                                                    cccAAGCGCGCTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCATTCAGTCCAGCCACGCCAAACGGCCCtt                 >  1:366089/1‑140 (MQ=255)

                                                                                                                              cTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCATTCAGTCCAGCCACGCCAAACGGCCCTTCCCGCAGGgc       >  2:1055360/1‑140 (MQ=255)

                                                                                                                              cTGTCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCATTCAGTCCAGCCACGCCAAACGGCCCTTCCCGCAGGgc       >  2:884/1‑140 (MQ=255)

                                                                                                                                 tCATTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCATTCAGTCCAGCCACGCCAAACGGCCCTTCCCGCAGGGCGca    >  1:811557/1‑140 (MQ=255)

                                                                                                                                   aTTACGAGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCATTCAGTCCAGCCACGCCAAACGGCCCTTCCCGTAGGGCGcaca  <  1:961226/140‑1 (MQ=255)

TCGTGCTCTGGCAAGCGACTCGGTTGCACAGACTTAATATTGCCGTCCGGCATCGCAAACCAGACGCGCGTCCCCTGCCCGCGATCCAGCGCAATACCGATATCGCCATTAAACAACCCAAGCGCGCTGTCATTACGGGCAATCATCACCGGTCGACCTTCGTACCAACGAGAGTGCGGATGACGATGAATTTTGCGCTTCTGTTGCATAAACTGCTCAATTCGCTCATTCAGTCCAGCCACGCCAAACGGCCCTTCCCGCAGGGCGCACA  >  NC_000913/2950870‑2951140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A