Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 116 98.7 3298914 99.2% 3272522 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 705,972 (T)9→8 intergenic (+82/‑121) nagE → / → glnS N‑acetylglucosamine specific PTS enzyme IIABC component/glutamine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913705,9640T.98.3% 253.8 / ‑1.8 59intergenic (+74/‑129)nagE/glnSN‑acetylglucosamine specific PTS enzyme IIABC component/glutamine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base . (25/33);  total (26/33)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

TTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTG  >  NC_000913/705825‑706099
                                                                                                                                           |                                                                                                                                       
ttGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCt                                                                                                                                         <  2:913803/140‑1 (MQ=255)
          aaGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCttttttttcat                                                                                                                               <  2:1103645/140‑4 (MQ=255)
          aaGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCttttttttcat                                                                                                                               <  1:417249/140‑4 (MQ=255)
             cTCAGGGCCATATTGTGGCGGGGGAAACACCCCCGTATGAAAACAAAAAAAAAAATGCTTTTTTCCTTATGCGGCGCCTGTGGGGCGCCCCCCACAAAAACAAAAGGGGGGGGACTTCTCTTCCGCctttttttttttaa                                                                                                                            >  2:1395056/1‑135 (MQ=255)
               cAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCatcaatc                                                                                                                         <  1:677375/140‑1 (MQ=255)
                aGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCatcaatca                                                                                                                        <  1:146008/140‑1 (MQ=255)
                aGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCatcaatca                                                                                                                        <  1:229170/140‑1 (MQ=255)
                  ggCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAatcatc                                                                                                                      <  2:290586/140‑1 (MQ=255)
                      atatTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCa                                                                                                                  <  2:130996/140‑1 (MQ=255)
                      atatTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCa                                                                                                                  <  2:128002/140‑1 (MQ=255)
                          tgtgGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAAt                                                                                                              <  2:694428/140‑1 (MQ=255)
                          tgtgGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAAt                                                                                                              <  2:1347775/140‑1 (MQ=255)
                          tgtgGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAAt                                                                                                              <  1:1495220/140‑1 (MQ=255)
                            tgGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATcc                                                                                                            <  1:495830/140‑1 (MQ=255)
                                      aaCACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGAtt                                                                                                  >  1:1312086/1‑140 (MQ=255)
                                      aaCACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGAtt                                                                                                  >  1:570755/1‑140 (MQ=255)
                                                tATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTa                                                                                        >  2:1257777/1‑140 (MQ=255)
                                                 aTGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTaa                                                                                       >  2:530859/1‑140 (MQ=255)
                                                 aTGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTaa                                                                                       <  1:791475/140‑1 (MQ=255)
                                                     aaTCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTaaaaaa                                                                                   >  1:159113/1‑140 (MQ=255)
                                                        cAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTa                                                                                >  2:668154/1‑140 (MQ=255)
                                                          aaaaGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAAc                                                                              <  1:588791/140‑1 (MQ=255)
                                                          aaaaGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAAc                                                                              <  1:1505462/140‑1 (MQ=255)
                                                          aaaaGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑CTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAAc                                                                              <  1:1190219/140‑1 (MQ=255)
                                                            aaGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAg                                                                            <  1:1368099/140‑1 (MQ=255)
                                                                 aTCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGt                                                                        >  1:53705/1‑140 (MQ=255)
                                                                   cTGCTTTATGCCTGTTGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAg                                                                     >  1:716402/1‑140 (MQ=255)
                                                                    tGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGc                                                                    <  1:859115/140‑1 (MQ=255)
                                                                         tATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTc                                                               <  1:1121126/140‑1 (MQ=255)
                                                                         tATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTc                                                               >  1:1467901/1‑140 (MQ=255)
                                                                          aTGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTcc                                                              <  2:750194/140‑1 (MQ=255)
                                                                          aTGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTcc                                                              <  2:838309/140‑1 (MQ=255)
                                                                           tGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTccc                                                             <  1:822150/140‑1 (MQ=255)
                                                                           tGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTccc                                                             >  2:1039441/1‑140 (MQ=255)
                                                                            gCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAACCTAACAGTTGTCAGCCTGTCCCg                                                            <  2:1312086/140‑1 (MQ=255)
                                                                                gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTta                                                        >  2:1091634/1‑140 (MQ=255)
                                                                                    cGACGCTTGCGCGACGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCATTAATCCCCATAATCCTTTTTAGATTATCAACTTTCAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAg                                                    >  2:1287369/1‑140 (MQ=255)
                                                                                           tGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATAc                                             <  1:877267/140‑1 (MQ=255)
                                                                                            gAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACg                                            <  2:159113/140‑1 (MQ=255)
                                                                                            gAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACg                                            >  2:107908/1‑140 (MQ=255)
                                                                                                 tCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGtt                                       >  2:1117164/1‑140 (MQ=255)
                                                                                                 tCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGtt                                       >  2:217153/1‑140 (MQ=255)
                                                                                                 tCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGtt                                       >  1:6781/1‑140 (MQ=255)
                                                                                                  cGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTta                                      >  1:473886/1‑140 (MQ=255)
                                                                                                  cGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTta                                      <  1:678020/140‑1 (MQ=255)
                                                                                                            aaTGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGttt                            >  1:283071/1‑140 (MQ=255)
                                                                                                            aaTGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGttt                            <  1:1257777/140‑1 (MQ=255)
                                                                                                                 cAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCt                       >  1:141285/1‑140 (MQ=255)
                                                                                                                  aaGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCtt                      >  1:565070/1‑140 (MQ=255)
                                                                                                                   aGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCttt                     >  2:389006/1‑140 (MQ=255)
                                                                                                                      ggTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAg                  <  1:1395056/140‑1 (MQ=255)
                                                                                                                      ggTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAg                  >  2:776392/1‑140 (MQ=255)
                                                                                                                       gTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAgg                 <  1:231976/140‑1 (MQ=255)
                                                                                                                       gTGGAGATCTTCTCTGCCGC‑‑TTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGa                >  2:351573/1‑140 (MQ=255)
                                                                                                                           agaTCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATc             >  1:708979/1‑140 (MQ=255)
                                                                                                                               cttctCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACg         <  1:89253/140‑1 (MQ=255)
                                                                                                                               cttctCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACg         <  2:6781/140‑1 (MQ=255)
                                                                                                                               cttctCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACg         <  1:977100/140‑1 (MQ=255)
                                                                                                                               cttctCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACg         <  1:187882/140‑1 (MQ=255)
                                                                                                                                  ctctGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGAtg      >  2:931441/1‑140 (MQ=255)
                                                                                                                                  ctctGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGAtg      >  2:664523/1‑140 (MQ=255)
                                                                                                                                  ctctGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCATGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGAtg      >  1:85421/1‑140 (MQ=255)
                                                                                                                                     tGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGAtgagt   <  1:107908/140‑1 (MQ=255)
                                                                                                                                      gCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGAtgagtg  <  1:600524/140‑1 (MQ=255)
                                                                                                                                      gCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGAtgagtg  <  1:1039441/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
TTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTG  >  NC_000913/705825‑706099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A