Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 116 98.7 3298914 99.2% 3272522 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,553,284 T→C C20C (TGT→TGC)  hemF → coproporphyrinogen III oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,553,2840TC100.0% 248.1 / NA 66C20C (TGT→TGC) hemFcoproporphyrinogen III oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/33);  total (33/33)

ATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGTCAACTTTTCGCATGTC  >  NC_000913/2553153‑2553419
                                                                                                                                   |                                                                                                                                       
aTTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCt                                                                                                                                 <  1:1381753/140‑1 (MQ=255)
  tGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGa                                                                                                                               <  2:220971/140‑1 (MQ=255)
   gCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGAc                                                                                                                              >  2:297964/1‑140 (MQ=255)
         ggCGAGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGAccgccgg                                                                                                                        >  1:1349448/1‑139 (MQ=255)
           cGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCg                                                                                                                      <  2:437620/140‑1 (MQ=255)
            gTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGa                                                                                                                     <  1:1183320/140‑1 (MQ=255)
            gTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGa                                                                                                                     <  1:1244837/140‑1 (MQ=255)
            gTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGa                                                                                                                     <  1:595946/140‑1 (MQ=255)
              gATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATg                                                                                                                   >  1:727119/1‑140 (MQ=255)
                    ttATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAg                                                                                                             >  1:1564159/1‑140 (MQ=255)
                       tttCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAAt                                                                                                          <  2:343702/140‑1 (MQ=255)
                       tttCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAAt                                                                                                          <  2:1435451/140‑1 (MQ=255)
                       tttCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAAt                                                                                                          <  2:617014/140‑1 (MQ=255)
                       tttCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAAt                                                                                                          <  1:314691/140‑1 (MQ=255)
                         tCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAAttt                                                                                                        >  1:1504875/1‑140 (MQ=255)
                         tCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAAttt                                                                                                        >  1:912650/1‑140 (MQ=255)
                         tCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAAttt                                                                                                        >  1:180907/1‑140 (MQ=255)
                         tCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAAttt                                                                                                        >  2:1444587/1‑140 (MQ=255)
                           cACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGt                                                                                                      <  1:42389/140‑1 (MQ=255)
                              tGGTTCGACAACCAGAAAGCACATTCGAAAAAGTGTTAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGa                                                                                                   <  2:870177/140‑1 (MQ=255)
                              tGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGa                                                                                                   >  1:1517809/1‑140 (MQ=255)
                              tGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGa                                                                                                   <  1:25289/140‑1 (MQ=255)
                              tGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGa                                                                                                   <  1:836384/140‑1 (MQ=255)
                              tGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGa                                                                                                   <  1:1290240/140‑1 (MQ=255)
                                     aCAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGt                                                                                            >  1:1520848/1‑140 (MQ=255)
                                     aCAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGt                                                                                            >  2:1456043/1‑140 (MQ=255)
                                          cAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCa                                                                                       >  1:1009197/1‑140 (MQ=255)
                                          cAGAAAGCACATTCGAAAAAACGGTTAGTTATGAAACCCGACGCACACCTGGTTAAACAGCTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCa                                                                                       >  2:1279105/1‑140 (MQ=255)
                                                    aTTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCt                                                                             >  1:1492434/1‑140 (MQ=255)
                                                    aTTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCt                                                                             >  2:59450/1‑140 (MQ=255)
                                                      tCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTgg                                                                           >  1:773119/1‑140 (MQ=255)
                                                           aaaGCGTTAAGTTATGAAACCCGACGCAGACTAGGTTAAGCAGTTTCTGCCCAACCCTCAGGATACGAATTGCCAGCAGCTGACCGCCGTCGATGGTGCAGCATTTGTCGAAGATAGTTGGCAGCGCGAAGCTggaggcg                                                                      <  2:1161836/140‑1 (MQ=255)
                                                           aaaGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTggcggcg                                                                      <  1:804129/140‑1 (MQ=255)
                                                           aaaGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTggcggcg                                                                      <  2:1564159/140‑1 (MQ=255)
                                                            aaGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTggcggcgg                                                                     <  1:647692/140‑1 (MQ=255)
                                                              gCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGAT‑GCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGcggcggcgg                                                                  <  1:1117051/140‑1 (MQ=255)
                                                                  tAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCggcgggcg                                                               >  1:611344/1‑140 (MQ=255)
                                                                      ttATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGt                                                           <  1:1249462/140‑1 (MQ=255)
                                                                         tGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCgg                                                        >  1:919698/1‑140 (MQ=255)
                                                                         tGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCgg                                                        >  2:561018/1‑140 (MQ=255)
                                                                         tGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCgg                                                        <  2:1213994/140‑1 (MQ=255)
                                                                         tGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCgg                                                        <  2:1052316/140‑1 (MQ=255)
                                                                         tGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCgg                                                        <  1:1624606/140‑1 (MQ=255)
                                                                          gAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCggg                                                       <  1:930361/140‑1 (MQ=255)
                                                                           aaaCCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCgggg                                                      >  2:176958/1‑139 (MQ=255)
                                                                            aaCCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGgtg                                                     >  2:1403396/1‑140 (MQ=255)
                                                                            aaCCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGgtg                                                     <  2:840167/140‑1 (MQ=255)
                                                                                cGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGc                                                 >  1:630068/1‑140 (MQ=255)
                                                                                 gACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCg                                                <  1:1359135/140‑1 (MQ=255)
                                                                                 gACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCg                                                <  2:414323/140‑1 (MQ=255)
                                                                                   cGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTa                                              >  1:1130295/1‑140 (MQ=255)
                                                                                         ccAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAAtggtg                                        >  1:845386/1‑140 (MQ=255)
                                                                                            ggTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGgtggtg                                     >  2:633057/1‑140 (MQ=255)
                                                                                            ggTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGgtggtg                                     >  2:465402/1‑140 (MQ=255)
                                                                                             gTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGgtgt                                    >  1:1089407/1‑140 (MQ=255)
                                                                                                  aCAGTTTCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCg                               >  2:1429321/1‑140 (MQ=255)
                                                                                                      tttCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAAca                           >  2:123828/1‑140 (MQ=255)
                                                                                                        tCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAAcagg                         <  2:727119/140‑1 (MQ=255)
                                                                                                        tCTGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAAcagg                         <  2:919698/140‑1 (MQ=255)
                                                                                                          tGCTCAACCTTCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAAcaggca                       <  2:845386/140‑1 (MQ=255)
                                                                                                                    tCAGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGGAATGGGGGGGGTTTCGAACAGGCAGGCGTCAACt             >  2:26457/1‑140 (MQ=255)
                                                                                                                      aGGATACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGTCAACttt           >  1:1159155/1‑140 (MQ=255)
                                                                                                                          tACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGTCAACTTTTCGc       <  2:1492434/140‑1 (MQ=255)
                                                                                                                          tACGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGTCAACTTTTCGc       <  2:538929/140‑1 (MQ=255)
                                                                                                                           aCGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGTCAACTTTTCGCa      >  2:1026131/1‑140 (MQ=255)
                                                                                                                           aCGATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGTCAACTTTTCGCa      >  1:842520/1‑140 (MQ=255)
                                                                                                                             gATTTGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGTCAACTTTTCGCATg    >  2:149351/1‑140 (MQ=255)
                                                                                                                              aTTTGCCAGCGGCTGACCGACGTCGATGGCGCAGAAGTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGGGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGTCAACTTTTCGCATGt   <  1:549585/140‑1 (MQ=255)
                                                                                                                               tttGCCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGTCAACTTTTCGCATGTc  <  2:630068/140‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                       
ATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGTCAACTTTTCGCATGTC  >  NC_000913/2553153‑2553419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A