Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 116 98.7 3298914 99.2% 3272522 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,517,815 T→C E224E (GAA→GAG)  aroB ← 3‑dehydroquinate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,517,8150TC100.0% 213.4 / NA 58E224E (GAA→GAG) aroB3‑dehydroquinate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/27);  total (31/27)

CAGCGACCGCTTCACCATGTAACCAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCC  >  NC_000913/3517679‑3517946
                                                                                                                                        |                                                                                                                                   
cAGCGACCGCTTCACCATGTAACCAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTca                                                                                                                                  >  2:938474/1‑140 (MQ=255)
cAGCGACCGCTTCACCATGTAACCAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTca                                                                                                                                  >  1:1410949/1‑140 (MQ=255)
cAGCGACCGCTTCACCATGTAACCAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTca                                                                                                                                  >  1:342165/1‑140 (MQ=255)
 aGCGACCGCTTCACCATGTAACCAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTcac                                                                                                                                 >  1:816784/1‑140 (MQ=255)
  gCGACCGCTTCACCATGTAACCAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTcaca                                                                                                                                >  1:1541606/1‑140 (MQ=255)
     aCCGCTTCACCATGTAACCAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCacaaca                                                                                                                             >  1:935861/1‑140 (MQ=255)
     aCCGCTTCACCATGTAACCAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCacaaca                                                                                                                             <  2:1476566/140‑1 (MQ=255)
     aCCGCTTCACCATGTAACCAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCacaaca                                                                                                                             >  1:167159/1‑140 (MQ=255)
        gCTTCACCATGTAACCAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACg                                                                                                                          <  2:1387671/140‑1 (MQ=255)
             aCCATGTAACCAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGaa                                                                                                                     >  2:127593/1‑140 (MQ=255)
             aCCATGTAACCAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGaa                                                                                                                     <  2:1203549/140‑1 (MQ=255)
               cATGTAACCAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATa                                                                                                                   <  2:701583/140‑1 (MQ=255)
               cATGTAACCAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATa                                                                                                                   <  2:154642/140‑1 (MQ=255)
                    aaCCAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTa                                                                                                              >  1:335123/1‑140 (MQ=255)
                    aaCCAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTa                                                                                                              >  1:412226/1‑140 (MQ=255)
                      ccAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACg                                                                                                            >  1:171213/1‑140 (MQ=255)
                      ccAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACg                                                                                                            >  1:1381086/1‑140 (MQ=255)
                       cAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCTCGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGc                                                                                                           <  2:1059497/140‑1 (MQ=255)
                       cAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGc                                                                                                           <  2:1571401/140‑1 (MQ=255)
                        aaTTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGcc                                                                                                          >  2:1024918/1‑140 (MQ=255)
                                 aCCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGa                                                                                                 >  1:1547565/1‑140 (MQ=255)
                                 aCCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGa                                                                                                 >  1:122715/1‑140 (MQ=255)
                                 aCCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGa                                                                                                 <  2:432339/140‑1 (MQ=255)
                                 aCCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGa                                                                                                 <  2:1407778/140‑1 (MQ=255)
                                     cATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGt                                                                                             >  1:350401/1‑140 (MQ=255)
                                        ttCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCa                                                                                          <  2:365187/140‑1 (MQ=255)
                                                 aaTGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGcaaca                                                                                 >  1:1443054/1‑140 (MQ=255)
                                                 aaTGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGcaaca                                                                                 >  1:1538997/1‑140 (MQ=255)
                                                    ggCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACg                                                                              <  2:19485/140‑1 (MQ=255)
                                                              aaGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGAtt                                                                    <  2:171213/140‑1 (MQ=255)
                                                                    tgtCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTc                                                              >  1:1202332/1‑140 (MQ=255)
                                                                    tgtCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTc                                                              >  1:167287/1‑140 (MQ=255)
                                                                    tgtCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAACTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTc                                                              >  1:439396/1‑140 (MQ=255)
                                                                        ccAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTccagc                                                          <  2:935861/140‑1 (MQ=255)
                                                                        ccAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTccagc                                                          <  2:342165/140‑1 (MQ=255)
                                                                         cAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTccagcc                                                         >  2:780213/1‑140 (MQ=255)
                                                                         cAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTccagcc                                                         >  1:1260569/1‑140 (MQ=255)
                                                                                aGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGTGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTaaa                                                  >  1:249513/1‑140 (MQ=255)
                                                                                       cACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCa                                           <  2:1381086/140‑1 (MQ=255)
                                                                                       cACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCa                                           >  1:1291769/1‑140 (MQ=255)
                                                                                       cACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCa                                           <  2:167159/140‑1 (MQ=255)
                                                                                       cACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGGCCAGACGCAACAACGCATCCAGATTCTCTTCCCGCCAGTTAAAAAACGCa                                           >  2:1176879/1‑140 (MQ=255)
                                                                                         cGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCAcc                                         <  2:335123/140‑1 (MQ=255)
                                                                                            aaCCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTc                                      >  1:1426393/1‑140 (MQ=255)
                                                                                            aaCCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTc                                      <  2:367559/140‑1 (MQ=255)
                                                                                            aaCCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTc                                      <  2:1431391/140‑1 (MQ=255)
                                                                                            aaCCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTc                                      >  1:720128/1‑140 (MQ=255)
                                                                                                 ggTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGaa                                 >  1:320212/1‑140 (MQ=255)
                                                                                                 ggTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGaa                                 >  1:1408657/1‑140 (MQ=255)
                                                                                                   tttCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGaata                               <  2:96705/140‑1 (MQ=255)
                                                                                                     tCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGaataat                             <  1:1024918/140‑1 (MQ=255)
                                                                                                       gcgcTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGc                           <  2:618666/140‑1 (MQ=255)
                                                                                                        cgcTCGTCGGCGGCGACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGcc                          <  2:1410949/140‑1 (MQ=255)
                                                                                                                    gcgACAACTTCTGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGAc              <  2:1443054/140‑1 (MQ=255)
                                                                                                                               tGCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGcc   >  2:566770/1‑140 (MQ=255)
                                                                                                                                gCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGccc  <  2:1291769/140‑1 (MQ=255)
                                                                                                                                gCCTTCAGCTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGccc  <  2:350401/140‑1 (MQ=255)
                                                                                                                                gCCTTCAACTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGccc  <  2:439396/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                   
CAGCGACCGCTTCACCATGTAACCAATTGCCATACCCCATTTCAGCTTCAATGGCATGACCAAAGGTGTGTCCCAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCC  >  NC_000913/3517679‑3517946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A