Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F28 I1 R1 120 56.0 1898522 99.4% 1887130 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 485,347 (GA)4→3 coding (272‑273/1194 nt) acrA ← multidrug efflux pump membrane fusion lipoprotein AcrA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913485,3400A.96.9% 256.1 / 2.8 64coding (280/1194 nt)acrAmultidrug efflux pump membrane fusion lipoprotein AcrA
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base . (32/30);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01
*NC_000913485,3410G.96.9% 264.3 / 2.3 64coding (279/1194 nt)acrAmultidrug efflux pump membrane fusion lipoprotein AcrA
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base . (32/30);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

TTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATC  >  NC_000913/485201‑485467
                                                                                                                                           ||                                                                                                                              
ttACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATa                                                                                                                                 <  1:866731/140‑1 (MQ=255)
    tGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagaga                                                                                                                             <  1:514141/140‑1 (MQ=255)
       tGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagagagac                                                                                                                          <  1:735778/140‑2 (MQ=255)
        gTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagagagaca                                                                                                                         >  1:802167/1‑138 (MQ=255)
            tGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGacacctg                                                                                                                     >  1:374779/1‑137 (MQ=255)
             gAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGacacctgc                                                                                                                    <  2:802167/140‑5 (MQ=255)
              aGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTTGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagagaga                                                                                                                           >  1:222421/1‑132 (MQ=255)
              aGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTTGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagagaga                                                                                                                           <  2:222421/132‑1 (MQ=255)
              aGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCt                                                                                                                 >  2:792199/1‑140 (MQ=255)
              aGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCt                                                                                                                 >  2:592418/1‑140 (MQ=255)
                  ccGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGa                                                                                                             <  2:374779/140‑1 (MQ=255)
                  ccGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGa                                                                                                             <  2:100209/140‑1 (MQ=255)
                   cGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGAt                                                                                                            >  1:678096/1‑140 (MQ=255)
                    gAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATg                                                                                                           <  2:286849/140‑1 (MQ=255)
                     aGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGAt                                                                                                            >  2:653538/1‑138 (MQ=255)
                     aGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGAt                                                                                                            <  1:653538/138‑1 (MQ=255)
                     aGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGt                                                                                                          >  2:194522/1‑140 (MQ=255)
                     aGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGt                                                                                                          >  1:311818/1‑140 (MQ=255)
                        aGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATg                                                                                                           <  2:109358/136‑1 (MQ=255)
                        aGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATg                                                                                                           >  1:109358/1‑136 (MQ=255)
                          tttCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTa                                                                                                     >  1:539112/1‑140 (MQ=255)
                          tttCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTa                                                                                                     >  2:298789/1‑140 (MQ=255)
                           ttCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTAc                                                                                                    <  1:460560/140‑1 (MQ=255)
                            tCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTAcc                                                                                                   <  2:539112/140‑1 (MQ=255)
                            tCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTAcc                                                                                                   <  2:927580/140‑1 (MQ=255)
                                        tCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAAtt                                                                                       >  2:901879/1‑140 (MQ=255)
                                         cACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTa                                                                                      <  1:592418/140‑1 (MQ=255)
                                         cACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTa                                                                                      <  2:837418/140‑1 (MQ=255)
                                          aCCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTAc                                                                                     >  2:534374/1‑140 (MQ=255)
                                             gTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCt                                                                                  <  1:194522/140‑1 (MQ=255)
                                              tCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCtt                                                                                 <  2:311818/140‑1 (MQ=255)
                                                     cgcgATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATa                                                                          >  2:389738/1‑140 (MQ=255)
                                                      gcgATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATaa                                                                         <  1:534374/140‑1 (MQ=255)
                                                      gcgATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATaa                                                                         <  2:207078/140‑1 (MQ=255)
                                                        gATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATc                                                                       <  1:792199/140‑1 (MQ=255)
                                                        gATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATc                                                                       <  1:389738/140‑1 (MQ=255)
                                                                    aGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGa                                                           >  1:772522/1‑140 (MQ=255)
                                                                    aGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGa                                                           >  1:344266/1‑140 (MQ=255)
                                                                    aGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGa                                                           >  1:353640/1‑140 (MQ=255)
                                                                    aGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGa                                                           >  2:169212/1‑140 (MQ=255)
                                                                      ccTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAgg                                                         >  2:750800/1‑140 (MQ=255)
                                                                         gggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACg                                                      <  1:298789/140‑1 (MQ=255)
                                                                          ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGa                                                     >  2:36938/1‑140 (MQ=255)
                                                                              ttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACtt                                                 <  2:678096/140‑1 (MQ=255)
                                                                                cGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCt                                               >  1:694270/1‑140 (MQ=255)
                                                                                   cAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCg                                            <  1:691870/140‑1 (MQ=255)
                                                                                        cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg                                       <  1:114249/140‑1 (MQ=255)
                                                                                          ccTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGt                                     <  2:289887/140‑1 (MQ=255)
                                                                                          ccTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGt                                     <  2:344266/140‑1 (MQ=255)
                                                                                                 cACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACt                              >  1:618795/1‑140 (MQ=255)
                                                                                                 cACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACt                              >  1:16649/1‑140 (MQ=255)
                                                                                                     gTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTgg                            <  2:276358/138‑1 (MQ=255)
                                                                                                     gTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTgg                            >  1:276358/1‑138 (MQ=255)
                                                                                                     gTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTg                          >  1:581647/1‑140 (MQ=255)
                                                                                                     gTCGTATGTCGCCTGATAGGTCGCAGGATCAA‑CTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGc                         >  2:779068/1‑140 (MQ=255)
                                                                                                         tATGTCGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAc                      >  1:542411/1‑140 (MQ=255)
                                                                                                            gTCGCCTGATAGGTCGCAGGATCAA‑CTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCgg                  <  1:779068/140‑1 (MQ=255)
                                                                                                              cGCCTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGa                 >  2:889092/1‑140 (MQ=255)
                                                                                                                ccTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAg               <  2:581647/140‑1 (MQ=255)
                                                                                                                ccTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAg               <  2:694270/140‑1 (MQ=255)
                                                                                                                ccTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAg               >  1:279942/1‑140 (MQ=255)
                                                                                                                ccTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAg               <  1:901879/140‑1 (MQ=255)
                                                                                                                ccTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAg               >  2:274907/1‑140 (MQ=255)
                                                                                                                ccTGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAg               >  1:658207/1‑140 (MQ=255)
                                                                                                                  tGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCt             >  2:307864/1‑140 (MQ=255)
                                                                                                                  tGATAGGTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCt             <  1:307864/140‑1 (MQ=255)
                                                                                                                        gTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAGCTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtg       >  1:80487/1‑140 (MQ=255)
                                                                                                                        gTCGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtg       >  1:305410/1‑140 (MQ=255)
                                                                                                                          cGCAGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg     <  2:618795/140‑1 (MQ=255)
                                                                                                                             aGGATCAATCTGAT‑‑AGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATc  <  1:750800/140‑1 (MQ=255)
                                                                                                                                           ||                                                                                                                              
TTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATC  >  NC_000913/485201‑485467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A