Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	120	56.0	1898522	99.4%	1887130	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,621,890	T→C	S232P (TCA→CCA)	purM →	phosphoribosylformylglycinamide cyclo‑ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,621,890	0	T	C	100.0%	129.5 / NA	36	S232P (TCA→CCA)	purM	phosphoribosylformylglycinamide cyclo‑ligase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/16); total (20/16)

AGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCA  >  NC_000913/2621755‑2622020

aGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGt                                                                                                                                >  2:501265/1‑140 (MQ=255)

   ggTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCt                                                                                                                             <  2:907741/140‑1 (MQ=255)

      ccACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGa                                                                                                                          >  2:909787/1‑140 (MQ=255)

      ccACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGa                                                                                                                          >  2:891922/1‑140 (MQ=255)

               aaCGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGttgattga                                                                                                                 >  2:219025/1‑140 (MQ=255)

               aaCGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGttgattga                                                                                                                 >  1:566263/1‑140 (MQ=255)

               aaCGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTAATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGttgattga                                                                                                                 >  1:772722/1‑140 (MQ=255)

                aCGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGaa                                                                                                                <  1:909787/140‑1 (MQ=255)

                      aTTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTc                                                                                                          >  2:45469/1‑140 (MQ=255)

                      aTTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTc                                                                                                          >  2:895451/1‑140 (MQ=255)

                      aTTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTc                                                                                                          >  2:393061/1‑140 (MQ=255)

                       ttCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCg                                                                                                         <  1:219025/140‑1 (MQ=255)

                         cGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGAt                                                                                                       <  1:891922/140‑1 (MQ=255)

                                                           tgATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCt                                                                     >  2:406275/1‑140 (MQ=255)

                                                            gATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCtt                                                                    <  1:406275/140‑1 (MQ=255)

                                                                  cAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGa                                                              >  1:128/1‑140 (MQ=255)

                                                                   aaaCCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGaa                                                             >  1:742672/1‑140 (MQ=255)

                                                                                 gATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGt                                               >  2:390587/1‑140 (MQ=255)

                                                                                     gTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTg                                           <  2:742672/140‑1 (MQ=255)

                                                                                       aaGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGcc                                         <  2:128/140‑1 (MQ=255)

                                                                                       aaGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGcc                                         <  1:895451/140‑1 (MQ=255)

                                                                                       aaGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGcc                                         <  1:45469/140‑1 (MQ=255)

                                                                                       aaGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGcc                                         <  1:393061/140‑1 (MQ=255)

                                                                                        aGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCa                                        >  2:139992/1‑140 (MQ=255)

                                                                                                    atcatcTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACt                               >  2:185907/1‑137 (MQ=255)

                                                                                                    atcatcTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACt                               <  1:185907/137‑1 (MQ=255)

                                                                                                       atcTGCTGGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCa                         <  1:139992/140‑1 (MQ=255)

                                                                                                         ctgctgGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACt                               <  2:183713/132‑1 (MQ=255)

                                                                                                         ctgctgGCACCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACt                               >  1:183713/1‑132 (MQ=255)

                                                                                                                 aCCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATtgatg               >  2:211470/1‑140 (MQ=255)

                                                                                                                 aCCGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATtgatg               >  2:559029/1‑140 (MQ=255)

                                                                                                                  ccGACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATtgatga              >  1:866452/1‑140 (MQ=255)

                                                                                                                    gACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAAt            >  1:913950/1‑140 (MQ=255)

                                                                                                                    gACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAAt            <  2:566263/140‑1 (MQ=255)

                                                                                                                    gACCCGCATTTACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAAt            <  1:390587/140‑1 (MQ=255)

                                                                                                                              tACGTGAAGCCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCa  <  1:211470/140‑1 (MQ=255)

AGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCA  >  NC_000913/2621755‑2622020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A