Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	120	56.0	1898522	99.4%	1887130	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,874,431	G→A	A330V (GCG→GTG)	cysN ←	sulfate adenylyltransferase subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,874,431	0	G	A	100.0%	166.3 / NA	49	A330V (GCG→GTG)	cysN	sulfate adenylyltransferase subunit 1
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/22); total (27/22)

TAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACGCTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGTCACGATCCGCGCGACGTTTGATT  >  NC_000913/2874302‑2874563

tAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTgc                                                                                                                            >  2:562195/1‑140 (MQ=255)

    gAATGCCATCAACACGCGCGCGCGTCTTCTTCCCGGAATTTTTGATGTCGTAACTCTGCCCGGGAAAAAGCGGTTGTTCCGCCATCCATACCACACCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTgccgcc                                                                                                                        <  2:492396/140‑1 (MQ=255)

         ccATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCcagcag                                                                                                                   >  1:21674/1‑140 (MQ=255)

            tCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCcagcagcag                                                                                                                >  1:456724/1‑140 (MQ=255)

             cAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGa                                                                                                               >  1:818770/1‑140 (MQ=255)

              aaCCCGCGCGCGCGTCTTCTTCCCGGCATTTTTGATGGCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCCCCATCCATACCACACCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGAt                                                                                                              <  2:386837/140‑1 (MQ=255)

                 ccgcgcgcgggTTTTCTTACCGGAATTTTTGTTGTCGTACCCCTCCCGGGGAGAAGGGGGCTGCTCCCCCACCCTCACCACATCCCCGGCGGCGCTCTCCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGc                                                                                                           <  2:456724/139‑1 (MQ=255)

                  cgcgcgcgcgTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGcc                                                                                                          <  2:21674/140‑1 (MQ=255)

                   gcgcgcgcgTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATCCCACACCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCa                                                                                                         <  1:858752/140‑1 (MQ=255)

                   gcgcgcgcgTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCa                                                                                                         <  1:562195/140‑1 (MQ=255)

                    cgcgcgcgTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCAc                                                                                                        >  1:480625/1‑140 (MQ=255)

                    cgcgcgcgTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCAc                                                                                                        >  1:720906/1‑140 (MQ=255)

                                   aCCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGAtct                                                                                         >  1:872357/1‑140 (MQ=255)

                                      ggCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCAt                                                                                      >  1:375281/1‑140 (MQ=255)

                                          aTTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATCCCACACCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGt                                                                                  <  2:771239/140‑1 (MQ=255)

                                                cattCGTAACTCCGCCCTGGAGAAAGCGGCTGTCCCGCCACCCTTCCCCCACCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCAc                                                                            <  2:790269/137‑1 (MQ=255)

                                                    cGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAgg                                                                        >  2:487058/1‑140 (MQ=255)

                                                     gTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAggg                                                                       >  2:258636/1‑140 (MQ=255)

                                                      tAACTCTGCCCTGGAGAAAGCCGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGt                                                                      <  1:754527/140‑1 (MQ=255)

                                                       aaCTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTg                                                                     <  1:487058/140‑1 (MQ=255)

                                                           ctGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCg                                                                 >  1:237727/1‑140 (MQ=255)

                                                             gCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCt                                                               >  1:746879/1‑140 (MQ=255)

                                                             gCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCt                                                               >  1:628916/1‑140 (MQ=255)

                                                              cccTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCtt                                                              >  1:10246/1‑140 (MQ=255)

                                                              cccTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCtg                                                              >  1:790269/1‑139 (MQ=255)

                                                                cgggAGAAGGCGGCTGTCCCGCCATCCATCCCACACCCCCCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTtct                                                            <  2:638335/138‑1 (MQ=255)

                                                                 tGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCCGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTtctc                                                           >  2:11069/1‑140 (MQ=255)

                                                                        aGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCaaa                                                    >  2:759775/1‑140 (MQ=255)

                                                                              tGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGcttc                                              <  1:759775/140‑1 (MQ=255)

                                                                                       ccccatACCACCTCCCCCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATc                                     <  2:36581/138‑1 (MQ=255)

                                                                                        tccatACCACATCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCa                                    >  2:498672/1‑140 (MQ=255)

                                                                                               cacaTCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCaa                             <  2:375281/140‑1 (MQ=255)

                                                                                                 caTCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCaaaa                           >  2:768797/1‑140 (MQ=255)

                                                                                                   tCCACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGt                         >  1:144989/1‑140 (MQ=255)

                                                                                                   ccccccGACGCGCTCTGCGCCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGt                         <  1:607830/136‑1 (MQ=255)

                                                                                                    ccACCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCATGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGTc                        >  1:292642/1‑140 (MQ=255)

                                                                                                      aCCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGTCAc                      >  2:359011/1‑140 (MQ=255)

                                                                                                      aCCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGTCAc                      >  2:1992/1‑140 (MQ=255)

                                                                                                      aCCGACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGGGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGTCAc                      >  2:553207/1‑140 (MQ=255)

                                                                                                         gACGCGCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGTCACGAt                   >  2:698099/1‑140 (MQ=255)

                                                                                                           cgcgCTCTGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGTCACGATcc                 >  1:58473/1‑140 (MQ=255)

                                                                                                              gcTCTGCACCGCCGGTAATACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGTCACGATCcgcg              <  1:803008/140‑1 (MQ=255)

                                                                                                                tccgCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGTCACGATCcgcgcg            <  1:11069/137‑1 (MQ=255)

                                                                                                                  tGCACCGCCGGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGTCACGATCCGCGCGAc          >  1:16981/1‑140 (MQ=255)

                                                                                                                      ccgccgGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGTCACGATCCGCGCGACGttt      <  2:480625/140‑1 (MQ=255)

                                                                                                                      ccgccgGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGTCACGATCCGCGCGACGttt      <  2:720906/140‑1 (MQ=255)

                                                                                                                      ccgccgGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGTCACGATCCGCGCGACGttt      <  2:10246/140‑1 (MQ=255)

                                                                                                                        gccgGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGTCACGATCCGCGCGACGTTTGa    <  2:16981/140‑1 (MQ=255)

                                                                                                                        gccgGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGGCACGATCCGCGCGACGTTTGa    <  1:553207/140‑1 (MQ=255)

                                                                                                                          cgGTAACACTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGTCACGATCCGCGCGACGTTTGAtt  <  1:768797/140‑1 (MQ=255)

TAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACGCTTCGTCTGCCGCCAGCAGCAGATCGCCACGGCTGATGTCGATCTCATCCGTCAGCACCAGGGTGATCGCTTCTCCGGCAAAGGCTTCTTCGCGATCACCATCAAAAGTCACGATCCGCGCGACGTTTGATT  >  NC_000913/2874302‑2874563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A