Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	120	56.0	1898522	99.4%	1887130	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,714,680	T→C	N572S (AAC→AGC)	bisC ←	biotin sulfoxide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,714,680	0	T	C	100.0%	163.9 / NA	47	N572S (AAC→AGC)	bisC	biotin sulfoxide reductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/26); total (21/26)

CCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCC  >  NC_000913/3714545‑3714810

ccAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTc                                                                                                                                >  2:435907/1‑140 (MQ=255)

ccAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTc                                                                                                                                >  1:637516/1‑140 (MQ=255)

  aGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCg                                                                                                                              <  1:435907/140‑1 (MQ=255)

   gggCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCgg                                                                                                                             <  1:887493/140‑1 (MQ=255)

   gggCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCgg                                                                                                                             <  1:752687/140‑1 (MQ=255)

     gCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCa                                                                                                                           >  1:21812/1‑140 (MQ=255)

        aTCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCAtct                                                                                                                        <  2:21812/140‑1 (MQ=255)

         tCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCAtctc                                                                                                                       >  2:909368/1‑140 (MQ=255)

         tCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCAtctc                                                                                                                       >  1:25699/1‑140 (MQ=255)

           cGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCaa                                                                                                                     <  1:909368/140‑1 (MQ=255)

              gTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTa                                                                                                                  >  1:733590/1‑140 (MQ=255)

                  ccGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTg                                                                                                              <  2:637516/140‑1 (MQ=255)

                  ccGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTg                                                                                                              <  1:60107/140‑1 (MQ=255)

                            cAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGc                                                                                                    <  1:897380/140‑1 (MQ=255)

                            cAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGc                                                                                                    >  2:249551/1‑140 (MQ=255)

                            cAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGc                                                                                                    >  1:645723/1‑140 (MQ=255)

                              aTACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCa                                                                                                  <  2:25699/140‑1 (MQ=255)

                                aCGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGa                                                                                                <  1:598194/140‑1 (MQ=255)

                                              tttCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATggc                                                                                  >  1:668739/1‑140 (MQ=255)

                                               ttCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATggcg                                                                                 <  2:668739/140‑1 (MQ=255)

                                                tCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATggcgg                                                                                <  2:475063/140‑1 (MQ=255)

                                                          cGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAAcc                                                                      <  2:421522/140‑1 (MQ=255)

                                                            cTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACctg                                                                    <  1:738218/140‑1 (MQ=255)

                                                            cTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACctg                                                                    <  1:942421/140‑1 (MQ=255)

                                                                           ggATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCcgcg                                                     <  1:783700/140‑1 (MQ=255)

                                                                            gATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGTAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCgcgc                                                    <  1:345166/140‑1 (MQ=255)

                                                                             aTGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCcgcg                                                     >  1:117156/1‑138 (MQ=255)

                                                                             aTGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCcgcg                                                     <  2:117156/138‑1 (MQ=255)

                                                                             aTGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCcgcg                                                     <  2:260751/138‑1 (MQ=255)

                                                                             aTGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCcgcg                                                     >  1:260751/1‑138 (MQ=255)

                                                                             aTGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCt                                                   >  1:481422/1‑140 (MQ=255)

                                                                             aTGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCt                                                   >  2:308320/1‑140 (MQ=255)

                                                                                   cAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCggg                                             <  2:733590/140‑1 (MQ=255)

                                                                                   cAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCggg                                             <  2:715979/140‑1 (MQ=255)

                                                                                     gCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCa                                           >  2:76630/1‑140 (MQ=255)

                                                                                         aTCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGt                                       >  1:659707/1‑140 (MQ=255)

                                                                                           cgcgGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTta                                     <  2:481422/140‑1 (MQ=255)

                                                                                                cAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATaaaa                                >  2:526154/1‑140 (MQ=255)

                                                                                                          cgaagcgaaTAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATaaaa                                <  2:71799/130‑1 (MQ=255)

                                                                                                          cgaagcgaaTAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATaaaa                                >  1:71799/1‑130 (MQ=255)

                                                                                                               cgaaTAAACCGCTCGCTGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTg                 <  2:659707/140‑1 (MQ=255)

                                                                                                                          ctcgctGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACttt      >  1:469891/1‑140 (MQ=255)

                                                                                                                          ctcgctGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACttt      >  1:831882/1‑140 (MQ=255)

                                                                                                                           tcgctGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACtttt     >  1:206333/1‑140 (MQ=255)

                                                                                                                             gctGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTttc   <  2:206333/140‑1 (MQ=255)

                                                                                                                             gctGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTttc   >  1:186432/1‑140 (MQ=255)

                                                                                                                              ctGTCCGGGCTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTttcc  <  2:469891/140‑1 (MQ=255)

CCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCC  >  NC_000913/3714545‑3714810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A