Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	120	56.0	1898522	99.4%	1887130	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,899,468	G→A	N370N (AAC→AAT)	yieL ←	putative hydrolase YieL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,899,468	0	G	A	100.0%	143.2 / NA	41	N370N (AAC→AAT)	yieL	putative hydrolase YieL
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/21); total (20/21)

AGCAGATGGTGTGCCGCGACACGGCTCATTTCCTGGTAATCTTCGGTAATGATTAATTTCATCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGGTTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGG  >  NC_000913/3899332‑3899600

aGCAGATGGTGTGCCGCGACACGGCTCATTTCCTGGTAATCTTCGGTAATGATTAATTTCATCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTc                                                                                                                                   >  1:421785/1‑140 (MQ=255)

   aGATGGTGTGCCGCGACACGGCTCATTTCCTGGTAATCTTCGGTAATGATTAATTTCATCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGa                                                                                                                                <  1:715644/140‑1 (MQ=255)

     aTGGTGTGCCGCGACACGGCTCATTTCCTGGTAATCTTCGGTAATGATTAATTTCATCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGAcc                                                                                                                              >  1:120818/1‑140 (MQ=255)

     aTGGTGTGCCGCGACACGGCTCATTTCCTGGTAATCTTCGGTAATGATTAATTTCATCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGAcc                                                                                                                              >  1:42728/1‑140 (MQ=255)

         tgtgCCGCGACACGGCTCATTTCCTGGTAATCTTCGGTAATGATTAATTTCATCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGa                                                                                                                          <  2:42728/140‑1 (MQ=255)

            gCCGCGACACGGCTCATTTCCTGGTAATCTTCGGTAATGATTAATTTCATCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGatat                                                                                                                       <  2:120818/140‑1 (MQ=255)

               gcgACACGGCTCATTTCCTGGTAATCTTCGGTAATGATTAATTTCATCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTcc                                                                                                                    >  2:405813/1‑140 (MQ=255)

                cgACACGGCTCATTTCCTGGTAATCTTCGGTAATGATTAATTTCATCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCt                                                                                                                   >  1:255491/1‑140 (MQ=255)

                  acacGGCTCATTTCCTGGTAATCTTCGGTAATGATTAATTTCATCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTgg                                                                                                                 <  2:369123/140‑1 (MQ=255)

                        cTCATTTCCTGGTAATCTTCGGTAATGATTAATTTCATCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGat                                                                                                                         >  1:296641/1‑126 (MQ=255)

                        cTCATTTCCTGGTAATCTTCGGTAATGATTAATTTCATCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGat                                                                                                                         <  2:296641/126‑1 (MQ=255)

                                  ggTAATCTTCGGTAATGATTAATTTCATCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAAttt                                                                                                 >  2:100168/1‑140 (MQ=255)

                                            ggTAATGATTAATTTCATCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTc                                                                                       >  1:644157/1‑140 (MQ=255)

                                                 tGATTAATTTCATCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCt                                                                                  <  1:34846/140‑1 (MQ=255)

                                                 tGATTAATTTCATCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCt                                                                                  >  2:473616/1‑140 (MQ=255)

                                                   aTTAATTTCATCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCa                                                                                >  1:178078/1‑140 (MQ=255)

                                                             tCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGcc                                                                      <  1:462497/140‑1 (MQ=255)

                                                                   tGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCGGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGa                                                                >  2:823992/1‑140 (MQ=255)

                                                                         attttatttAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCt                                                          >  1:509111/1‑140 (MQ=255)

                                                                          ttttatttAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCtt                                                         <  1:100168/140‑1 (MQ=255)

                                                                           tttatttaAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTg                                                        <  1:183906/140‑1 (MQ=255)

                                                                             tatttaAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTg                                                        <  1:387630/138‑1 (MQ=255)

                                                                             tatttaAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTg                                                        >  2:387630/1‑138 (MQ=255)

                                                                                ttaAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGt                                                   <  2:178078/140‑1 (MQ=255)

                                                                                    ataatTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACg                                               >  2:481988/1‑140 (MQ=255)

                                                                                     taatTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGa                                              <  1:473616/140‑1 (MQ=255)

                                                                                       atTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACa                                            <  1:656227/140‑1 (MQ=255)

                                                                                              tACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTAt                                     <  1:600539/140‑1 (MQ=255)

                                                                                              tACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATACCCTTGCCGGTTACGACATCTTTAt                                     <  1:481988/140‑1 (MQ=255)

                                                                                                  aaGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATcccc                                 <  2:255491/140‑1 (MQ=255)

                                                                                                        gCATAGGCTGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAAc                           >  2:252838/1‑140 (MQ=255)

                                                                                                                tGGTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTaa                   >  1:714920/1‑140 (MQ=255)

                                                                                                                 ggTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTGATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTaaa                  >  2:124973/1‑140 (MQ=255)

                                                                                                                 ggTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATccccc                                <  1:323677/126‑1 (MQ=255)

                                                                                                                 ggTCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATccccc                                >  2:323677/1‑126 (MQ=255)

                                                                                                                   tCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAAt                >  1:518668/1‑140 (MQ=255)

                                                                                                                   tCGCCAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTGTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAAt                >  2:628766/1‑140 (MQ=255)

                                                                                                                       cAGACATCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACg            <  1:291235/140‑1 (MQ=255)

                                                                                                                             tCCATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTg      <  2:644157/140‑1 (MQ=255)

                                                                                                                              ccATTTCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGc     <  1:252838/140‑1 (MQ=255)

                                                                                                                                 tttCATGATTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTgg  <  2:714920/140‑1 (MQ=255)

AGCAGATGGTGTGCCGCGACACGGCTCATTTCCTGGTAATCTTCGGTAATGATTAATTTCATCTTAATGCCTTATTTTATTTAAATAATTTCTGTACAAAGGCTGCATAGGCTGGTCGCCAGACATCCATTTCATGGTTCAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGG  >  NC_000913/3899332‑3899600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A