Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F28 I1 R1
|
106 |
90.3 |
3060316 |
99.2% |
3035833 |
139.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,734,543 |
(C)8→9 |
coding (492/732 nt) |
pgeF ← |
polyphenol oxidase YfiH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,734,535 | 1 | . | C | 88.0%
| 162.0
/ 15.0
| 50 | coding (500/732 nt) | pgeF | polyphenol oxidase YfiH |
| Reads supporting (aligned to +/- strand): ref base . (3/3); new base C (23/21); total (26/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 |
GCCGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCG > NC_000913/2734396‑2734669
|
gccgccGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCt > 2:1410893/1‑140 (MQ=255)
gccgAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑ccc > 2:1244389/1‑140 (MQ=255)
ccgAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑ccc‑c < 1:739616/140‑1 (MQ=255)
gAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑ccc‑ccc < 1:812911/140‑1 (MQ=255)
gAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑ccc‑ccc < 1:739439/140‑1 (MQ=255)
aaaTTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑ccc‑ccccc < 1:1193100/140‑1 (MQ=255)
aaaTTTGCGCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑ccc‑ccccc < 1:212090/140‑1 (MQ=255)
cAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGaa < 1:795937/140‑1 (MQ=255)
cacCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑CCC‑CCCCCACTTCGAAcgcg < 1:1526041/140‑1 (MQ=255)
aCGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑CCC‑CCCCCACTTCGAAagcgccggg > 2:675191/1‑136 (MQ=255)
cGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCa > 2:928894/1‑140 (MQ=255)
cGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTAGCTGTAGCGTCTACTGCCATAAACGCATCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCa > 2:841923/1‑140 (MQ=255)
gACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGc < 2:1035541/140‑1 (MQ=255)
gACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGc < 1:226799/140‑1 (MQ=255)
ccGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCt < 1:1133111/140‑1 (MQ=255)
ccGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCt < 1:1270275/140‑1 (MQ=255)
cGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTa > 2:86067/1‑140 (MQ=255)
aGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAgg < 1:1525094/140‑1 (MQ=255)
tAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGaa > 2:120535/1‑140 (MQ=255)
cAGATGCTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGGCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGa < 2:584799/140‑1 (MQ=255)
cAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGa > 2:482838/1‑140 (MQ=255)
aCTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATc > 1:283589/1‑140 (MQ=255)
aCCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑CCCACCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCaaaa > 1:1380153/1‑140 (MQ=255)
cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACa > 1:131779/1‑140 (MQ=255)
aTGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACa > 2:1394601/1‑140 (MQ=255)
aTGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAg > 2:911835/1‑140 (MQ=255)
aTGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAg > 1:809937/1‑140 (MQ=255)
tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg > 2:1100256/1‑140 (MQ=255)
tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg > 2:368041/1‑140 (MQ=255)
tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg > 1:114053/1‑140 (MQ=255)
tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg > 1:1476194/1‑140 (MQ=255)
gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGa > 2:445950/1‑140 (MQ=255)
gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGa > 2:1434515/1‑140 (MQ=255)
aatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCAAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAAcc > 1:886376/1‑140 (MQ=255)
atgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAAcc < 1:1394601/140‑1 (MQ=255)
atgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGt < 2:963148/140‑1 (MQ=255)
tgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGt < 1:899745/140‑1 (MQ=255)
aaaGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGtct > 1:1089939/1‑140 (MQ=255)
aGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGtctc > 1:1402167/1‑140 (MQ=255)
aGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTctct < 1:1473408/140‑1 (MQ=255)
aGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTctct < 1:845615/140‑1 (MQ=255)
cACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAg < 2:809937/140‑1 (MQ=255)
aCTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa < 1:1161038/140‑1 (MQ=255)
cTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa < 1:826098/140‑1 (MQ=255)
cTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa < 1:1336520/140‑1 (MQ=255)
cTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa < 1:622695/140‑1 (MQ=255)
ttGCTTTAGCGTCTACTGCCATAACCGCCTCGCGAACCTCCCCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa < 2:904438/140‑1 (MQ=255)
cGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca < 1:841923/140‑1 (MQ=255)
cGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca < 1:86067/140‑1 (MQ=255)
cGTCTACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca < 1:206785/140‑1 (MQ=255)
tACTGCCATAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGt < 1:1127369/140‑1 (MQ=255)
cATAAACGCCTCGCGAACCT‑‑CCCACCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCgcgc < 2:1380153/140‑1 (MQ=255)
tAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGCCCAATTGCCGGCCCTAACCAGGCGAGAAAATTTTCCGGATTAGCAGCAAAACAGGAAACCGTCTCTTCCAGCACCCCTCCGCACAGCCCACgcca > 2:1164983/1‑140 (MQ=255)
tAAACGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgccc > 2:329592/1‑139 (MQ=255)
aCGCCTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgccagcc < 2:283589/140‑1 (MQ=255)
ccTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCcagccagc < 1:1244389/140‑1 (MQ=255)
cTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCa > 1:540590/1‑140 (MQ=255)
cTCGCGAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGGCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCa > 2:447524/1‑140 (MQ=255)
cTCGCGAACCT‑‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCAt > 1:1155819/1‑140 (MQ=255)
cgcgAACCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATg > 2:599345/1‑140 (MQ=255)
aaCCTC‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAgcg < 1:911835/140‑1 (MQ=255)
|
GCCGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑‑CCC‑CCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCG > NC_000913/2734396‑2734669
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
