Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	672,440	T→C	I782V (ATC→GTC)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	672,440	0	T	C	100.0%	151.6 / NA	42	I782V (ATC→GTC)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/20); total (22/20)

CCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTT  >  NC_000913/672311‑672572

ccTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACAtcgccttcg                                                                                                                            <  1:378409/140‑1 (MQ=255)

ccTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACAtcgccttcg                                                                                                                            <  2:624307/140‑1 (MQ=255)

     tcttcCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCttt                                                                                                                       <  1:491780/140‑1 (MQ=255)

       ttcCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCa                                                                                                                     <  2:609552/140‑1 (MQ=255)

         ccGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGt                                                                                                                   >  2:113330/1‑140 (MQ=255)

         ccGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGt                                                                                                                   <  2:145624/140‑1 (MQ=255)

              gCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTg                                                                                                              >  2:386832/1‑140 (MQ=255)

                    aCCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAg                                                                                                        <  2:405535/140‑1 (MQ=255)

                            ggTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGc                                                                                                >  2:743965/1‑140 (MQ=255)

                             gTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCa                                                                                               >  1:183918/1‑140 (MQ=255)

                                   ttGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtgtg                                                                                         >  2:640006/1‑140 (MQ=255)

                                     ggCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCg                                                                                       <  1:163741/140‑1 (MQ=255)

                                      gCACGGACTTCACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCgg                                                                                      <  1:726853/140‑1 (MQ=255)

                                       cACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCggg                                                                                     <  2:346411/140‑1 (MQ=255)

                                            aCTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGaa                                                                                <  2:475474/140‑1 (MQ=255)

                                                     ttAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAg                                                                       >  1:976646/1‑140 (MQ=255)

                                                      tAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGaa                                                                                >  1:641347/1‑130 (MQ=255)

                                                      tAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGaa                                                                                <  2:641347/130‑1 (MQ=255)

                                                             caccacGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGa                                                               >  2:143236/1‑140 (MQ=255)

                                                             caccacGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGa                                                               >  1:763435/1‑140 (MQ=255)

                                                               ccacGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGAc                                                              <  1:863089/139‑1 (MQ=255)

                                                               ccacGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGAc                                                              >  2:863089/1‑139 (MQ=255)

                                                               ccacGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACa                                                             <  1:452540/140‑1 (MQ=255)

                                                                  cGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAg                                                                       <  2:149207/127‑1 (MQ=255)

                                                                  cGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAg                                                                       >  1:149207/1‑127 (MQ=255)

                                                                  cGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACg                                                          >  2:744291/1‑140 (MQ=255)

                                                                        gCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGTCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCcagc                                                    >  1:19830/1‑140 (MQ=255)

                                                                        gCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCcagc                                                    >  1:184296/1‑140 (MQ=255)

                                                                         cGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTCCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCcagca                                                   <  1:743965/140‑1 (MQ=255)

                                                                                tCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTc                                            <  2:495708/140‑1 (MQ=255)

                                                                                   tCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTg                                         >  1:485033/1‑140 (MQ=255)

                                                                                      cccaTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCAt                                      <  2:287864/139‑1 (MQ=255)

                                                                                        caTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCa                                    <  2:428726/140‑1 (MQ=255)

                                                                                        caTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCa                                    <  2:184296/140‑1 (MQ=255)

                                                                                           cGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCagag                                 >  1:785748/1‑140 (MQ=255)

                                                                                             cTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAgcg                               >  2:830392/1‑140 (MQ=255)

                                                                                             cTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAgcg                               >  1:749601/1‑140 (MQ=255)

                                                                                                  tCGTCAGCAACCGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATc                          >  1:467766/1‑140 (MQ=255)

                                                                                                            ccGGCCACGGCGCGTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCa                <  2:755766/140‑1 (MQ=255)

                                                                                                                      cgcgTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTg      >  1:99697/1‑140 (MQ=255)

                                                                                                                      cgcgTTGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTg      >  2:574993/1‑140 (MQ=255)

                                                                                                                          ttGTCGACATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCttt  >  1:649050/1‑140 (MQ=255)

CCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTT  >  NC_000913/672311‑672572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A