Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,080,959	C→T	R226H (CGC→CAC)	tktA ←	transketolase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,080,959	0	C	T	100.0%	159.2 / NA	47	R226H (CGC→CAC)	tktA	transketolase 1
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/21); total (26/21)

GCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAA  >  NC_000913/3080838‑3081095

gCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGt                                                                                                                        >  2:32286/1‑140 (MQ=255)

 cccAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTACACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTc                                                                                                                       >  2:425293/1‑140 (MQ=255)

         cgcACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGt                                                                                                               >  2:428787/1‑140 (MQ=255)

          gcACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGCTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTc                                                                                                              >  1:87878/1‑140 (MQ=255)

           cACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCg                                                                                                             <  1:855909/140‑1 (MQ=255)

                tGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTc                                                                                                        >  1:730567/1‑140 (MQ=255)

                tGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTc                                                                                                        >  1:497968/1‑140 (MQ=255)

                   gAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTcgcg                                                                                                     >  2:917704/1‑140 (MQ=255)

                     gTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGaa                                                                                                   >  1:748099/1‑140 (MQ=255)

                      tCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAAt                                                                                                  >  1:24816/1‑140 (MQ=255)

                                     ttGTTCGGGGAACCGAAACCGATGAGGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTa                                                                                   <  1:261457/140‑1 (MQ=255)

                                      tGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTaa                                                                                  >  2:363122/1‑140 (MQ=255)

                                             ggAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGa                                                                           >  1:332224/1‑140 (MQ=255)

                                              gAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGaa                                                                          <  2:300296/140‑1 (MQ=255)

                                                 ccgaaaccgaTGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACg                                                                       <  1:71694/140‑1 (MQ=255)

                                                 ccgaaaccgaTGATGGTTTGGCACATCAGCAGGAAAGGTTTTTCAGTCACTGCGCGCGCTTCTGCAACTGCGTGTTTGATAGATGCCGGGGCATGGCCGTCGATGTCGCGAATAACGGGCCAGCCGTAAGCTTCGAAACg                                                                       <  2:87878/140‑1 (MQ=255)

                                                            gatgGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGgtgt                                                            >  2:52304/1‑140 (MQ=255)

                                                              tgGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTgtcg                                                          >  2:636303/1‑140 (MQ=255)

                                                               ggTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTgtcgt                                                         >  1:246857/1‑140 (MQ=255)

                                                                      cacaTCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGaa                                                  <  2:797359/140‑1 (MQ=255)

                                                                       acaTCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAAc                                                 >  2:75720/1‑140 (MQ=255)

                                                                        caTCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAAcc                                                <  1:917704/140‑1 (MQ=255)

                                                                          tCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAg                                              >  2:503314/1‑140 (MQ=255)

                                                                           cagcagGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAAc                                                 >  1:737711/1‑136 (MQ=255)

                                                                           cagcagGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGc                                             >  1:1022845/1‑140 (MQ=255)

                                                                           cagcagGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGCCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAAc                                                 <  2:737711/136‑1 (MQ=255)

                                                                                   aaGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTg                                                    <  2:808282/125‑1 (MQ=255)

                                                                                   aaGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTg                                                    >  1:808282/1‑125 (MQ=255)

                                                                                      gTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGGCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGa                                  <  1:636303/140‑1 (MQ=255)

                                                                                       tttGTCAGTCACTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGAc                                 <  1:503314/140‑1 (MQ=255)

                                                                                           tcagtcaCTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATc                             <  1:898406/140‑1 (MQ=255)

                                                                                           tcagtcaCTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATc                             <  2:748099/140‑1 (MQ=255)

                                                                                           tcagtcaCTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATc                             <  2:24816/140‑1 (MQ=255)

                                                                                              gtcaCTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGa                                  >  1:432953/1‑132 (MQ=255)

                                                                                              gtcaCTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGa                                  <  2:432953/132‑1 (MQ=255)

                                                                                              gtcaCTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGAt                          <  1:52304/140‑1 (MQ=255)

                                                                                              gtcaCTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGAt                          <  1:379715/140‑1 (MQ=255)

                                                                                                caCTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAg                        >  2:508186/1‑140 (MQ=255)

                                                                                                caCTGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAg                        >  2:286381/1‑140 (MQ=255)

                                                                                                   tGCGCGCGCTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGaaa                     >  2:902991/1‑140 (MQ=255)

                                                                                                       cgcgcTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATc                             >  2:267986/1‑128 (MQ=255)

                                                                                                       cgcgcTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATc                             <  1:267986/128‑1 (MQ=255)

                                                                                                       cgcgcTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATAcc                 <  2:246857/140‑1 (MQ=255)

                                                                                                        gcgcTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCg                >  1:758300/1‑140 (MQ=255)

                                                                                                         cgcTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGt               >  1:618543/1‑140 (MQ=255)

                                                                                                         cgcTTCTTCTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGt               <  1:286381/140‑1 (MQ=255)

                                                                                                            ttcttcTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGt            <  2:497968/140‑1 (MQ=255)

                                                                                                            ttcttcTACTGCGTGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGt            <  1:902991/140‑1 (MQ=255)

                                                                                                                      gcgtGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCg       <  2:233491/135‑1 (MQ=255)

                                                                                                                      gcgtGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCg       >  1:233491/1‑135 (MQ=255)

                                                                                                                      gcgtGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGaa  >  2:393885/1‑140 (MQ=255)

GCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAA  >  NC_000913/3080838‑3081095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A