Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F28 I1 R1
|
138 |
62.6 |
2119710 |
99.4% |
2106991 |
138.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,216,058 |
A→G |
V145A (GTC→GCC) |
nfeF ← |
NADPH‑dependent ferric chelate reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,216,058 | 0 | A | G | 84.3%
| 131.4
/ 14.5
| 51 | V145A (GTC→GCC) | nfeF | NADPH‑dependent ferric chelate reductase |
| Reads supporting (aligned to +/- strand): ref base A (4/4); new base G (21/22); total (25/26) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATC > NC_000913/3215923‑3216192
|
ccATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATa < 1:391575/140‑1 (MQ=255)
aTCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACa > 1:975057/1‑140 (MQ=255)
tCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAg > 1:884859/1‑140 (MQ=255)
ggTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGa < 2:1018656/140‑1 (MQ=255)
gcgcGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATAc < 1:804696/140‑1 (MQ=255)
cgcgAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATAcg > 2:755188/1‑140 (MQ=255)
cgcgAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATAcg > 2:461665/1‑140 (MQ=255)
cgAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGa < 1:144123/134‑1 (MQ=255)
cgAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGa < 1:303364/134‑1 (MQ=255)
cgAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGa > 2:144123/1‑134 (MQ=255)
cgAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGa > 2:303364/1‑134 (MQ=255)
agaTAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTa > 2:804477/1‑140 (MQ=255)
aTAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAAt < 2:826200/140‑1 (MQ=255)
aaTCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCt > 1:444299/1‑140 (MQ=255)
aTCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCACAGGCATACAGCTGATACGCGTAATCtt < 1:99548/140‑1 (MQ=255)
aCAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGca > 2:586845/1‑140 (MQ=255)
cAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCgggcc < 2:444299/140‑4 (MQ=255)
cAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGcac < 2:701333/140‑1 (MQ=255)
aGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGcacc > 1:221661/1‑140 (MQ=255)
cGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGcac < 1:706678/136‑1 (MQ=255)
cGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGcac > 2:706678/1‑136 (MQ=255)
cGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCaccacc > 2:770831/1‑140 (MQ=255)
cACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACcgc > 2:956135/1‑140 (MQ=255)
cACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACcgc > 1:481145/1‑140 (MQ=255)
cACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACcgc > 1:335695/1‑140 (MQ=255)
aCCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGAcc > 1:1015275/1‑140 (MQ=255)
cAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTg < 1:11922/140‑1 (MQ=255)
cAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTg < 1:695247/140‑1 (MQ=255)
cAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTg < 2:486717/140‑1 (MQ=255)
aGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCt < 1:934195/138‑1 (MQ=255)
aGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCt > 2:934195/1‑138 (MQ=255)
aGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGc < 1:802573/140‑1 (MQ=255)
gcgcACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGcc < 2:858201/140‑1 (MQ=255)
aCTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCg > 2:103374/1‑140 (MQ=255)
aCTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCg > 2:624868/1‑140 (MQ=255)
tAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGcc < 1:803325/122‑1 (MQ=255)
tAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGcc > 2:803325/1‑122 (MQ=255)
cGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGt < 1:62292/140‑1 (MQ=255)
ttGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTg < 2:884859/140‑1 (MQ=255)
cTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCAt < 1:861726/140‑1 (MQ=255)
cGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGccc < 2:335695/140‑1 (MQ=255)
aGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCt < 1:586845/140‑1 (MQ=255)
gcggcgCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGa < 1:103374/140‑1 (MQ=255)
gcggcgCAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGa < 1:624868/140‑1 (MQ=255)
ggcgCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGAcc > 2:570708/1‑140 (MQ=255)
gcAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCa < 1:755188/140‑1 (MQ=255)
gcAATGCAGGCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCa < 1:630510/140‑1 (MQ=255)
ggCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTg > 2:789184/1‑140 (MQ=255)
ggCATTCCGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTg < 1:770831/140‑1 (MQ=255)
ccGGATTCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATaaa > 2:101687/1‑140 (MQ=255)
ggATTCAGCGCAGGCATACAGCTGATACGCGTAATCTTCGGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGa < 2:341016/140‑1 (MQ=255)
ttCATCGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAaat > 2:334396/1‑140 (MQ=255)
cGCAGGCATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAaatcaatc > 2:716152/1‑140 (MQ=255)
|
CCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGTTTAACGGCAAGTTTGCTCAACGTTTCCAGGCGGCGGCGCAATGCAGGCATTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATC > NC_000913/3215923‑3216192
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
