Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	72.5	2472046	99.4%	2457213	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,793,727	G→A	A128V (GCC→GTC)	btuE ←	thioredoxin/glutathione peroxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,793,727	0	G	A	100.0%	166.1 / NA	47	A128V (GCC→GTC)	btuE	thioredoxin/glutathione peroxidase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/25); total (22/25)

AATGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTG  >  NC_000913/1793594‑1793858

aaTGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGa                                                                                                                               >  1:15937/1‑140 (MQ=255)

 aTGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGaa                                                                                                                              >  1:515336/1‑140 (MQ=255)

  tGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAAt                                                                                                                             >  2:808376/1‑140 (MQ=255)

  tGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAAt                                                                                                                             >  1:1147502/1‑140 (MQ=255)

         ttCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGctct                                                                                                                      <  1:187402/140‑1 (MQ=255)

             ggCGTAATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTc                                                                                                                  >  1:1080299/1‑140 (MQ=255)

              gCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTcc                                                                                                                 >  2:1082169/1‑140 (MQ=255)

              gCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTcc                                                                                                                 >  1:303235/1‑140 (MQ=255)

                  cATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGgcg                                                                                                             >  2:385924/1‑140 (MQ=255)

                            gAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGt                                                                                                   <  1:1082169/140‑1 (MQ=255)

                              aaaaCGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCg                                                                                                 >  2:40025/1‑140 (MQ=255)

                                         tGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGca                                                                                      <  2:282606/140‑1 (MQ=255)

                                           aCTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGcaat                                                                                    <  2:515336/140‑1 (MQ=255)

                                           aCTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGaaat                                                                                    <  2:303235/140‑1 (MQ=255)

                                             ttttCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGcaatca                                                                                  <  1:438523/140‑1 (MQ=255)

                                               ttCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGcaatcaat                                                                                >  1:518723/1‑140 (MQ=255)

                                                  cGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAAtttt                                                                             <  1:40025/140‑1 (MQ=255)

                                                    tCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGAACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTg                                                                           <  2:1080299/140‑1 (MQ=255)

                                                      ccTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCCGCAATCAATTTTTGAt                                                                         >  2:446927/1‑140 (MQ=255)

                                                        tGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATAc                                                                       <  2:51388/140‑1 (MQ=255)

                                                           cAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGc                                                                    <  1:610529/140‑1 (MQ=255)

                                                              ccAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACGATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGa                                                                 >  2:844331/1‑140 (MQ=255)

                                                                aGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATg                                                               <  1:385924/140‑1 (MQ=255)

                                                                   aaTTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCg                                                            <  2:15937/140‑1 (MQ=255)

                                                                       tttCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCt                                                        >  1:274727/1‑140 (MQ=255)

                                                                        ttCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCtt                                                       <  1:808376/140‑1 (MQ=255)

                                                                        ttCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCtt                                                       <  2:67246/140‑1 (MQ=255)

                                                                        ttCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCCGCAATCAATTTTTGATACAGCGGATGGCGTCCtt                                                       <  1:446927/140‑1 (MQ=255)

                                                                         tCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTc                                                      <  2:971772/140‑1 (MQ=255)

                                                                         tCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTc                                                      <  2:484006/140‑1 (MQ=255)

                                                                              aTTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCAt                                                 <  1:844331/140‑1 (MQ=255)

                                                                                  cATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAAc                                             >  1:1066855/1‑140 (MQ=255)

                                                                                       aaTATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCaa                                        <  2:1147502/140‑1 (MQ=255)

                                                                                       aaTATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCaa                                        <  2:518723/140‑1 (MQ=255)

                                                                                                         cGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATc                                      <  2:204036/124‑1 (MQ=255)

                                                                                                         cGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATc                                      >  1:204036/1‑124 (MQ=255)

                                                                                                         cGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGa                      <  2:114006/140‑1 (MQ=255)

                                                                                                         cGGTGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTACTTCGCCATTCACGTCAATCTTACTGAACATCGGGa                      <  2:531816/140‑1 (MQ=255)

                                                                                                            tGCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACg                   >  1:821281/1‑140 (MQ=255)

                                                                                                             gCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATc                          <  2:342059/132‑1 (MQ=255)

                                                                                                             gCACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATc                          >  1:342059/1‑132 (MQ=255)

                                                                                                              cACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTc                 >  2:791979/1‑140 (MQ=255)

                                                                                                              cACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTc                 >  2:975935/1‑140 (MQ=255)

                                                                                                              cACGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTc                 >  2:1186369/1‑140 (MQ=255)

                                                                                                                cGGCCTTTGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCAc               <  1:791979/140‑1 (MQ=255)

                                                                                                                      ttGCTGACCATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCa         <  1:975935/140‑1 (MQ=255)

                                                                                                                             ccATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATgtggtg  >  2:133089/1‑140 (MQ=255)

                                                                                                                             ccATACGGACATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATgtggtg  >  1:934668/1‑140 (MQ=255)

AATGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTG  >  NC_000913/1793594‑1793858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A