Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 126 72.5 2472046 99.4% 2457213 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,000,854 A→G V170V (GTA→GTG)  xdhA → putative xanthine dehydrogenase molybdenum‑binding subunit XdhA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,000,8540AG98.2% 203.2 / NA 56V170V (GTA→GTG) xdhAputative xanthine dehydrogenase molybdenum‑binding subunit XdhA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (21/34);  minor base T (1/0);  total (22/34)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGT  >  NC_000913/3000719‑3000988
                                                                                                                                       |                                                                                                                                      
aCCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAgg                                                                                                                                    >  2:406002/1‑140 (MQ=255)
 cccGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAggg                                                                                                                                   >  2:937755/1‑140 (MQ=255)
 cccGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAggg                                                                                                                                   >  1:1215912/1‑140 (MQ=255)
 cccGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCC‑ACAAACAATCGATGCCGCCGACTACCAGGTGCAgggg                                                                                                                                  <  2:498422/140‑1 (MQ=255)
  ccGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAgggg                                                                                                                                  <  2:1215912/140‑1 (MQ=255)
  ccGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAgggg                                                                                                                                  <  2:109506/140‑1 (MQ=255)
  ccGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAgggg                                                                                                                                  <  1:46073/140‑1 (MQ=255)
  ccGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAgggg                                                                                                                                  <  1:674402/140‑1 (MQ=255)
   cGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGc                                                                                                                                 <  2:405994/140‑1 (MQ=255)
        tCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTAt                                                                                                                            <  1:406002/140‑1 (MQ=255)
        tCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTAt                                                                                                                            <  2:1002924/140‑1 (MQ=255)
           ccacGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAg                                                                                                                         <  1:1113015/140‑1 (MQ=255)
            cacGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGa                                                                                                                        >  2:1218381/1‑140 (MQ=255)
             acGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGAc                                                                                                                       <  1:414430/140‑1 (MQ=255)
                ccAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGAcccc                                                                                                                    >  2:1075818/1‑140 (MQ=255)
                ccAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGAcccc                                                                                                                    <  2:479154/140‑1 (MQ=255)
                  aGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCg                                                                                                                  <  2:468947/140‑1 (MQ=255)
                  aGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCg                                                                                                                  <  1:1203059/140‑1 (MQ=255)
                    aaGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGc                                                                                                                                 <  1:655226/123‑1 (MQ=255)
                    aaGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGc                                                                                                                                 >  2:655226/1‑123 (MQ=255)
                        ggcgCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTc                                                                                                            >  2:542053/1‑140 (MQ=255)
                            cTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACa                                                                                                        <  1:672688/140‑1 (MQ=255)
                                      aCGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATg                                                                                              <  1:1075818/140‑1 (MQ=255)
                                        gCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGa                                                                                            <  1:542053/140‑1 (MQ=255)
                                         cTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGaa                                                                                           >  2:663436/1‑140 (MQ=255)
                                          tGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACa                                                                                                        <  1:605076/126‑1 (MQ=255)
                                          tGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACa                                                                                                        >  2:605076/1‑126 (MQ=255)
                                                     aTAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCg                                                                               <  1:864622/140‑1 (MQ=255)
                                                     aTAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCg                                                                               <  2:813592/140‑1 (MQ=255)
                                                      tAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTTCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGc                                                                              >  2:1080250/1‑140 (MQ=255)
                                                      tAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGc                                                                              >  1:773138/1‑140 (MQ=255)
                                                      tAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGc                                                                              >  1:36658/1‑140 (MQ=255)
                                                          ggTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGc                                                                          <  2:773138/140‑1 (MQ=255)
                                                          ggTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGc                                                                          <  2:36658/140‑1 (MQ=255)
                                                           gTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCg                                                                         <  1:163093/140‑1 (MQ=255)
                                                           gTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCg                                                                         >  2:470796/1‑140 (MQ=255)
                                                            tGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGt                                                                        >  1:1088158/1‑140 (MQ=255)
                                                              gCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCg                                                                         <  2:902043/137‑1 (MQ=255)
                                                              gCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCg                                                                         >  1:902043/1‑137 (MQ=255)
                                                              gCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGtgg                                                                      >  1:32125/1‑140 (MQ=255)
                                                              gCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATCCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGCAAGCGTAACATCGCTGGCGtgg                                                                      >  2:278956/1‑140 (MQ=255)
                                                               cAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGtgga                                                                     <  1:663436/140‑1 (MQ=255)
                                                                aaTTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGtggat                                                                    <  2:32125/140‑1 (MQ=255)
                                                                       tGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGAt                                                             <  1:1218381/140‑1 (MQ=255)
                                                                             aaaGCACGATGTCGACGGGTAATGTCCAACAAGCAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTcg                                                       <  2:1088158/140‑1 (MQ=255)
                                                                                    gATGTCGACGGGTAATGTCCAACAAACAATCTATGCCGCCGACTACCAGGTGGAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTa                                                <  1:476096/140‑1 (MQ=255)
                                                                                                 aaTGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAg                                   <  1:793871/140‑1 (MQ=255)
                                                                                                  aTGTCCAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGc                                  >  1:157783/1‑140 (MQ=255)
                                                                                                      ccAACAAACAATCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCAccc                              <  1:470796/140‑1 (MQ=255)
                                                                                                         acaaacaaTCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGa                           >  2:69775/1‑140 (MQ=255)
                                                                                                               aaTCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGc                     >  2:561479/1‑140 (MQ=255)
                                                                                                               aaTCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGc                     >  2:306264/1‑140 (MQ=255)
                                                                                                                 tCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGACTTACCATCGTTTCCAGCAccc                              <  2:874747/129‑1 (MQ=255)
                                                                                                                 tCGATGCCGCCGACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCAccc                              >  1:874747/1‑129 (MQ=255)
                                                                                                                           cgACTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTcgcc         <  2:242080/140‑1 (MQ=255)
                                                                                                                             aCTACCAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTcgccgc       >  2:274412/1‑140 (MQ=255)
                                                                                                                                  cAGGTGCAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGtggt  <  1:1128036/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
ACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGT  >  NC_000913/3000719‑3000988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A