Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	72.5	2472046	99.4%	2457213	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,157,770	A→G	T41A (ACT→GCT)	yqhG →	DUF3828 domain‑containing protein YqhG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,157,770	0	A	G	100.0%	164.2 / NA	45	T41A (ACT→GCT)	yqhG	DUF3828 domain‑containing protein YqhG
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/23); total (22/23)

GAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTT  >  NC_000913/3157636‑3157896

gagGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGcc                                                                                                                           <  2:797111/140‑1 (MQ=255)

    tGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCtt                                                                                                                       >  2:481462/1‑140 (MQ=255)

    tGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCtt                                                                                                                       >  2:1190893/1‑140 (MQ=255)

         aaCAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGCCCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTAtttt                                                                                                                  <  2:1085161/140‑1 (MQ=255)

             gATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTg                                                                                                              <  2:934060/140‑1 (MQ=255)

             gATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTg                                                                                                              <  2:590723/140‑1 (MQ=255)

             gATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTg                                                                                                              >  1:590723/1‑140 (MQ=255)

              aTGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCt                                                                                                                              >  1:858101/1‑123 (MQ=255)

              aTGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGa                                                                                                             <  2:818845/140‑1 (MQ=255)

                        taCTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGagag                                                                                                   <  1:271945/140‑1 (MQ=255)

                        taCTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGagag                                                                                                   >  2:804096/1‑140 (MQ=255)

                           ttCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCgg                                                                                                <  1:964981/140‑1 (MQ=255)

                                tttttAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGAt                                                                                           >  2:928134/1‑140 (MQ=255)

                                         gcCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTgc                                                                                  <  1:1047109/140‑1 (MQ=255)

                                         gcCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTgc                                                                                  <  1:1190893/140‑1 (MQ=255)

                                         gcCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTgc                                                                                  <  1:1202011/140‑1 (MQ=255)

                                         gcCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTgc                                                                                  <  2:44061/140‑1 (MQ=255)

                                         gcCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTgc                                                                                  <  1:804096/140‑1 (MQ=255)

                                          cccTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTgcg                                                                                 <  2:58188/140‑1 (MQ=255)

                                               gCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGcgcg                                                                               >  1:141988/1‑137 (MQ=255)

                                               gCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGcgcg                                                                               <  2:141988/137‑1 (MQ=255)

                                                    ttttACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCTGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAAc                                                                       >  2:528227/1‑140 (MQ=255)

                                                    ttttACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGCCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAAc                                                                       >  2:237598/1‑140 (MQ=255)

                                                               acGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTAcc                                                            <  1:481462/140‑1 (MQ=255)

                                                                      gCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACg                                                     >  2:571381/1‑140 (MQ=255)

                                                                       ccccccTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGa                                                    >  1:290745/1‑140 (MQ=255)

                                                                       ccccccTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGa                                                    >  1:24662/1‑140 (MQ=255)

                                                                                 aGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTAccc                                                           >  1:620291/1‑123 (MQ=255)

                                                                                 aGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTAccc                                                           <  2:620291/123‑1 (MQ=255)

                                                                                  gACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGc                                         >  1:263911/1‑140 (MQ=255)

                                                                                   aCCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCt                                        >  1:656461/1‑140 (MQ=255)

                                                                                     cGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACATGCGCTTACCCTGAACGACAATCTTACGCTGc                                      >  1:1221797/1‑140 (MQ=255)

                                                                                                    ccGGCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATtggct                       >  2:674229/1‑140 (MQ=255)

                                                                                                      ggCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACATGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATtggctgg                     <  2:1221797/140‑1 (MQ=255)

                                                                                                      ggCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATtggctgg                     <  1:655341/140‑1 (MQ=255)

                                                                                                      ggCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATtggctgg                     <  1:687785/140‑1 (MQ=255)

                                                                                                       gCATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATtggctggc                    <  2:554496/140‑1 (MQ=255)

                                                                                                        cATATTTATCAGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTggctggct                   <  2:656461/140‑1 (MQ=255)

                                                                                                                  aGAACTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGAt         <  2:24662/140‑1 (MQ=255)

                                                                                                                     aCTATAAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCg      >  1:852873/1‑140 (MQ=255)

                                                                                                                       tataAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGt    >  1:148434/1‑140 (MQ=255)

                                                                                                                         taAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGttt  >  2:531235/1‑140 (MQ=255)

                                                                                                                         taAATCAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGttt  >  2:1071248/1‑140 (MQ=255)

                                                                                                                              cAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGAtt              >  2:664180/1‑123 (MQ=255)

                                                                                                                              cAGATGCCGCTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGAtt              <  1:664180/123‑1 (MQ=255)

GAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATATTTATCAGAACTATAAATCAGATGCCACTGCCCCTTATTTTGGTGAAACCGGAGAGCGGGCGATAACTTCTGCGCGTATTCAACAGGCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTT  >  NC_000913/3157636‑3157896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A