Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	108	95.2	3177646	99.2%	3152224	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,977,802	T→C	A366A (GCA→GCG)	lysA ←	diaminopimelate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,977,802	0	T	C	99.1%	427.6 / ‑4.7	113	A366A (GCA→GCG)	lysA	diaminopimelate decarboxylase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (77/35); total (78/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 113 total aligned reads displayed.

TCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTG  >  NC_000913/2977665‑2977938

tcGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGc                                                                                                                                        >  1:893464/1‑140 (MQ=255)

   aTGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCtt                                                                                                                                     <  2:657044/140‑1 (MQ=255)

   aTGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCtt                                                                                                                                     <  1:829152/140‑1 (MQ=255)

      gTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCAc                                                                                                                                  >  1:809003/1‑140 (MQ=255)

      gTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCAc                                                                                                                                  >  2:333110/1‑140 (MQ=255)

         tGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTc                                                                                                                               <  2:1333225/140‑1 (MQ=255)

            cGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCgg                                                                                                                            <  1:1263426/140‑1 (MQ=255)

                ggCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAg                                                                                                                        >  2:913836/1‑140 (MQ=255)

                 gCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAgg                                                                                                                       >  2:521388/1‑140 (MQ=255)

                   gAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGgcg                                                                                                                     <  2:586693/140‑1 (MQ=255)

                   gAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGgcg                                                                                                                     <  2:717523/140‑1 (MQ=255)

                    aaTCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGgcgc                                                                                                                    <  2:1302041/140‑1 (MQ=255)

                     aTCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGcgcg                                                                                                                   <  1:407127/140‑1 (MQ=255)

                           cgcgCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTc                                                                                                             <  1:1481380/140‑1 (MQ=255)

                               ccTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACa                                                                                                         >  2:333574/1‑140 (MQ=255)

                                 tGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACAtt                                                                                                       <  2:117908/140‑1 (MQ=255)

                                   aCCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGAAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTc                                                                                                     >  2:824952/1‑140 (MQ=255)

                                    ccATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTcc                                                                                                    >  1:894405/1‑140 (MQ=255)

                                        tATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCt                                                                                                <  2:905209/140‑1 (MQ=255)

                                             aaCAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCctg                                                                                           >  1:342437/1‑140 (MQ=255)

                                             aaCAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCctg                                                                                           >  1:181266/1‑140 (MQ=255)

                                              aCAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCctgc                                                                                          <  2:683775/140‑1 (MQ=255)

                                              aCAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCctgc                                                                                          <  2:1350893/140‑1 (MQ=255)

                                               cAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCctgct                                                                                         >  2:485286/1‑140 (MQ=255)

                                                          gTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACa                                                                              <  1:591595/140‑1 (MQ=255)

                                                              cAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGc                                                                          >  1:537943/1‑140 (MQ=255)

                                                               aGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCCCCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGcc                                                                         <  2:25804/140‑1 (MQ=255)

                                                                 cGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCg                                                                       <  2:580026/140‑1 (MQ=255)

                                                                 cGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCg                                                                       >  2:904208/1‑140 (MQ=255)

                                                                 cGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCg                                                                       <  2:50338/140‑1 (MQ=255)

                                                                 cGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCg                                                                       >  2:1017947/1‑140 (MQ=255)

                                                                 cGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCg                                                                       <  2:578438/140‑1 (MQ=255)

                                                                 cGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCg                                                                       >  2:1309466/1‑140 (MQ=255)

                                                                  ggACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGa                                                                      >  2:1436825/1‑140 (MQ=255)

                                                                   gACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGAt                                                                     <  2:1085898/140‑1 (MQ=255)

                                                                   gACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGAt                                                                     <  1:814065/140‑1 (MQ=255)

                                                                   gACGGCTATTGTAGTTGGAGGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGAt                                                                     <  2:181266/140‑1 (MQ=255)

                                                                    aCGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGAtt                                                                    >  1:645535/1‑140 (MQ=255)

                                                                    aCGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGAtt                                                                    >  1:1344513/1‑140 (MQ=255)

                                                                           ttGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATaa                                                             <  1:132458/140‑1 (MQ=255)

                                                                           ttGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATaa                                                             >  2:1547491/1‑140 (MQ=255)

                                                                                  tGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCg                                                      >  1:176761/1‑140 (MQ=255)

                                                                                  tGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCg                                                      >  1:382284/1‑140 (MQ=255)

                                                                                  tGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCg                                                      >  2:395056/1‑140 (MQ=255)

                                                                                   ggATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCgg                                                     >  1:561066/1‑140 (MQ=255)

                                                                                     aTGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGcg                                                   >  2:195259/1‑140 (MQ=255)

                                                                                     aTGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGcg                                                   >  1:542404/1‑140 (MQ=255)

                                                                                     aTGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGcg                                                   >  2:910767/1‑140 (MQ=255)

                                                                                     aTGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGcg                                                   >  1:423862/1‑140 (MQ=255)

                                                                                      tGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGcga                                                  >  2:854708/1‑140 (MQ=255)

                                                                                        aCATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGcgacg                                                <  1:1008915/140‑1 (MQ=255)

                                                                                        aCATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGcgacg                                                >  1:551700/1‑140 (MQ=255)

                                                                                                gcgcCATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTc                                        <  2:1070133/140‑1 (MQ=255)

                                                                                                   ccATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCAc                                     >  1:366475/1‑140 (MQ=255)

                                                                                                   ccATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCAc                                     >  1:390092/1‑140 (MQ=255)

                                                                                                    cATATGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCAcc                                    >  2:763933/1‑140 (MQ=255)

                                                                                                       atGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGtt                                 <  2:1344513/140‑1 (MQ=255)

                                                                                                       atGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGtt                                 >  2:483148/1‑140 (MQ=255)

                                                                                                        tGCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTg                                >  2:359765/1‑140 (MQ=255)

                                                                                                         gCCCCTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTgg                               >  1:20087/1‑140 (MQ=255)

                                                                                                            ccTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGgcgc                            <  1:333574/140‑1 (MQ=255)

                                                                                                             cTGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGcgcg                           <  1:243104/140‑1 (MQ=255)

                                                                                                              tGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCgt                          >  2:955046/1‑140 (MQ=255)

                                                                                                              tGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCgt                          >  2:261584/1‑140 (MQ=255)

                                                                                                              tGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCgt                          >  1:1352416/1‑140 (MQ=255)

                                                                                                              tGTATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCgt                          >  1:1192524/1‑140 (MQ=255)

                                                                                                                tATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCgtgt                        >  1:1128408/1‑140 (MQ=255)

                                                                                                                tATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCgtgt                        >  2:665090/1‑140 (MQ=255)

                                                                                                                tATCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCgtgt                        >  1:968282/1‑140 (MQ=255)

                                                                                                                  tCATGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTc                      >  1:94200/1‑140 (MQ=255)

                                                                                                                     tGCAGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCag                   >  1:231340/1‑140 (MQ=255)

                                                                                                                     tGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCag                   >  2:889596/1‑140 (MQ=255)

                                                                                                                     tGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCag                   >  1:1473192/1‑140 (MQ=255)

                                                                                                                     tGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCag                   >  1:410038/1‑140 (MQ=255)

                                                                                                                     tGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCag                   >  1:640357/1‑140 (MQ=255)

                                                                                                                     tGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCag                   >  1:144672/1‑140 (MQ=255)

                                                                                                                     tGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCag                   >  1:1067487/1‑140 (MQ=255)

                                                                                                                     tGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCag                   >  2:1160840/1‑140 (MQ=255)

                                                                                                                     tGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCag                   >  2:1557233/1‑140 (MQ=255)

                                                                                                                     tGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCag                   >  2:18342/1‑140 (MQ=255)

                                                                                                                     tGCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCag                   >  1:1275461/1‑140 (MQ=255)

                                                                                                                      gCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCaga                  <  1:785181/140‑1 (MQ=255)

                                                                                                                      gCAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCaga                  >  1:1409444/1‑140 (MQ=255)

                                                                                                                       cAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCagag                 >  1:1197141/1‑140 (MQ=255)

                                                                                                                       cAGTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCagag                 >  1:316071/1‑140 (MQ=255)

                                                                                                                         gTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGaa               <  2:809003/140‑1 (MQ=255)

                                                                                                                         gTACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGaa               <  2:164213/140‑1 (MQ=255)

                                                                                                                          tACCAGATAATCACCCGCTTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGAAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAAc              >  1:1400253/1‑140 (MQ=255)

                                                                                                                          tACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAAc              >  1:874292/1‑140 (MQ=255)

                                                                                                                          tACCAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCCGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAAc              >  2:1072846/1‑140 (MQ=255)

                                                                                                                            ccAGATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGa            >  1:573721/1‑140 (MQ=255)

                                                                                                                               gATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGCCGGTTTCCACCGTTGGCCCGTGTTCCAGAGAACGACCa         >  2:216513/1‑140 (MQ=255)

                                                                                                                               gATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCa         >  2:446523/1‑140 (MQ=255)

                                                                                                                               gATAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCa         >  2:1363785/1‑140 (MQ=255)

                                                                                                                                aTAATCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCAt        >  2:564713/1‑140 (MQ=255)

                                                                                                                                  aaTCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCa      >  1:1034545/1‑140 (MQ=255)

                                                                                                                                  aaTCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCa      <  1:824952/140‑1 (MQ=255)

                                                                                                                                    tCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCGTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGc    >  2:1272066/1‑140 (MQ=255)

                                                                                                                                    tCACCCGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGc    >  1:38312/1‑140 (MQ=255)

                                                                                                                                      aCCCGCCTTCACTTCCGGCAAGGCGCGGGGTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTg  >  2:751631/1‑140 (MQ=255)

TCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTG  >  NC_000913/2977665‑2977938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A