Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,634,642	C→T	G82S (GGC→AGC)	tfaQ ←	Qin prophage; putative tail fiber assembly protein TfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,634,642	0	C	T	100.0%	100.7 / NA	46	G82S (GGC→AGC)	tfaQ	Qin prophage; putative tail fiber assembly protein TfaQ
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/20); total (26/20)

TCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCT  >  NC_000913/1634510‑1634778

tCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGcc                                                                                                                                   <  2:173137/140‑1 (MQ=17)

    gAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGt                                                                                                                               >  2:341407/1‑140 (MQ=17)

    gAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTAAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGt                                                                                                                               >  2:340827/1‑140 (MQ=17)

     aaCAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTc                                                                                                                              >  2:456385/1‑140 (MQ=17)

       cAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCAt                                                                                                                            >  2:193955/1‑140 (MQ=17)

                          tCCTTCACCCAGGCTGTGCCGCTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTTACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCgatg                                                                                                         <  2:786880/140‑1 (MQ=12)

                               cACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCtt                                                                                                    >  1:615875/1‑140 (MQ=17)

                                    aGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACg                                                                                               >  1:624144/1‑140 (MQ=17)

                                     ggCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACga                                                                                              >  2:454559/1‑140 (MQ=17)

                                       cTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCgatga                                                                                                        >  1:369355/1‑128 (MQ=17)

                                       cTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCgatga                                                                                                        <  2:369355/128‑1 (MQ=18)

                                         gtgCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATg                                                                                          >  2:133467/1‑140 (MQ=17)

                                          tgCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGc                                                                                         >  1:260483/1‑140 (MQ=17)

                                           gCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGcc                                                                                        <  2:260483/140‑1 (MQ=17)

                                           gCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGcc                                                                                        >  1:579203/1‑140 (MQ=17)

                                               ttCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGa                                                                                    <  2:615875/140‑1 (MQ=17)

                                                tCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGAt                                                                                   >  2:378215/1‑140 (MQ=17)

                                                  cACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGa                                                                                 <  1:632816/140‑1 (MQ=17)

                                                  cACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGa                                                                                 <  2:557339/140‑1 (MQ=17)

                                                    cTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACt                                                                               >  1:50522/1‑140 (MQ=17)

                                                    cTTCTGAAACTCCCCCTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACt                                                                               >  1:377966/1‑140 (MQ=12)

                                                         gAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcatca                                                                          <  1:193955/140‑1 (MQ=17)

                                                         gAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcatca                                                                          <  1:378215/140‑1 (MQ=17)

                                                         gAAACTCCCCCTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcatca                                                                          <  2:377966/140‑1 (MQ=17)

                                                          aaaCTCCCCTTCCGGCGATAACCAGGTAACATTTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACAATGACtcatca                                                                          <  2:470937/140‑1 (MQ=255)

                                                            aCTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTg                                                                       >  1:48894/1‑140 (MQ=17)

                                                                 ccTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGaa                                                                  >  2:717028/1‑140 (MQ=17)

                                                                 ccTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGaa                                                                  >  1:244449/1‑140 (MQ=17)

                                                                                  ggTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagccag                                                 >  2:571142/1‑140 (MQ=17)

                                                                                  ggTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagccag                                                 <  2:624144/140‑1 (MQ=17)

                                                                                             tCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATc                                       >  2:550202/1‑140 (MQ=17)

                                                                                              ccGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCt                                      >  1:452287/1‑140 (MQ=17)

                                                                                                      ggACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGc                              <  2:244449/140‑1 (MQ=17)

                                                                                                      ggACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGc                              <  1:550202/140‑1 (MQ=17)

                                                                                                              ttCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCgg                      >  1:206025/1‑140 (MQ=17)

                                                                                                                           aaCGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTgg         <  2:366512/140‑1 (MQ=17)

                                                                                                                           aaCGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTgg         <  2:822485/140‑1 (MQ=17)

                                                                                                                            aCGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGc        <  1:340827/140‑1 (MQ=17)

                                                                                                                             cgcgTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCa       >  2:631048/1‑140 (MQ=17)

                                                                                                                             cgcgTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCa       >  2:675423/1‑140 (MQ=18)

                                                                                                                               cgTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGa     >  2:455181/1‑140 (MQ=17)

                                                                                                                                gTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGttt              >  2:238676/1‑130 (MQ=14)

                                                                                                                                gTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGttt              <  1:238676/130‑1 (MQ=17)

                                                                                                                                 tCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTg          <  1:793575/133‑1 (MQ=14)

                                                                                                                                 tCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTg          >  2:793575/1‑133 (MQ=11)

                                                                                                                                  cGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCt  <  2:452287/140‑1 (MQ=11)

TCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACA‑TTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCT  >  NC_000913/1634510‑1634778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A