Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,047,787	C→T	T950T (ACC→ACT)	yeeJ →	inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,047,787	0	C	T	100.0%	172.6 / NA	48	T950T (ACC→ACT)	yeeJ	inverse autotransporter adhesin
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/20); total (28/20)

CTGACCAGTTTGAAAAATGGTGATTATAGGGTTACGGCCTCTGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAA  >  NC_000913/2047650‑2047921

cTGACCAGTTTGAAAAATGGTGATTATAGGGTTACGGCCTCTGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGt                                                                                                                                      >  1:299230/1‑140 (MQ=255)

     cAGTTTGAAAAATGGTGATTATAGGGTTACGGCCTCTGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCa                                                                                                                                 >  1:616464/1‑140 (MQ=255)

           gAAAAATGGTGATTATAGGGTTACGGCCTCTGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAg                                                                                                                           <  1:274391/140‑1 (MQ=255)

           gAAAAATGGTGATTATAGGGTTACGGCCTCTGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAg                                                                                                                           <  2:724436/140‑1 (MQ=255)

              aaaTGGTGATTATAGGGTTACGGCCTCTGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTc                                                                                                                        <  2:303724/140‑1 (MQ=255)

                aTGGTGATTATAGGGTTACGGCCTCTGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCg                                                                                                                      <  2:253387/140‑1 (MQ=255)

                                tACGGCCTCTGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAatatat                                                                                                              >  1:321462/1‑132 (MQ=255)

                                tACGGCCTCTGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAatatat                                                                                                              <  2:321462/132‑1 (MQ=255)

                                tACGGCCTCTGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCaa                                                                                                      >  1:411401/1‑140 (MQ=255)

                                 aCGGCCTCTGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAAc                                                                                                     >  2:858805/1‑140 (MQ=255)

                                 aCGGCCTCTGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAAc                                                                                                     >  2:404165/1‑140 (MQ=255)

                                  cGGCCTCTGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCg                                                                                                                      <  1:383245/122‑1 (MQ=255)

                                  cGGCCTCTGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCg                                                                                                                      >  2:383245/1‑122 (MQ=255)

                                  cGGCCTCTGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAAcc                                                                                                    >  2:230152/1‑140 (MQ=255)

                                  cGGCCTCTGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAAcc                                                                                                    >  2:584320/1‑140 (MQ=255)

                                  cGGCCTCTGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAAcc                                                                                                    >  1:763687/1‑140 (MQ=255)

                                       tctGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCa                                                                                               >  2:297648/1‑140 (MQ=255)

                                              gCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATaaa                                                                                        <  2:411401/140‑1 (MQ=255)

                                                 ctGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTg                                                                                                 >  1:71243/1‑128 (MQ=255)

                                                 ctGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTg                                                                                                 <  2:71243/128‑1 (MQ=255)

                                                    gTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGc                                                                                  <  1:584320/140‑1 (MQ=255)

                                                    gTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGc                                                                                  <  1:858805/140‑1 (MQ=255)

                                                    gTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGc                                                                                  <  1:230152/140‑1 (MQ=255)

                                                        ccAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAAcc                                                                              <  1:297648/140‑1 (MQ=255)

                                                            gCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACt                                                                          <  2:616464/140‑1 (MQ=255)

                                                              tAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTaa                                                                        >  2:486287/1‑140 (MQ=255)

                                                                    aCAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATa                                                                  <  2:299230/140‑1 (MQ=255)

                                                                      aGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATaaa                                                                >  1:768272/1‑140 (MQ=255)

                                                                         tGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGaa                                                             <  2:768272/140‑1 (MQ=255)

                                                                         tGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGaa                                                             <  1:404165/140‑1 (MQ=255)

                                                                                  tCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTtc                                                    >  1:77823/1‑140 (MQ=255)

                                                                                        aTCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCtgtg                                              >  2:762906/1‑140 (MQ=255)

                                                                                                  tgctgcCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAacgacg                                    >  2:229358/1‑140 (MQ=255)

                                                                                                  tgctgcCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAacgacg                                    >  2:853742/1‑140 (MQ=255)

                                                                                                    ctgcCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACTACGTc                                  >  1:249611/1‑140 (MQ=255)

                                                                                                    ctgcCCTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTc                                  <  1:761464/140‑1 (MQ=255)

                                                                                                         cTGACCCTCAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCaag                             >  1:459802/1‑140 (MQ=255)

                                                                                                                 cAGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTtct                     <  1:853742/140‑1 (MQ=255)

                                                                                                                  aGTGTGCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTGAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTtctc                    >  2:753779/1‑140 (MQ=255)

                                                                                                                      tgCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGAtt                >  1:38083/1‑140 (MQ=255)

                                                                                                                       gCCTTCAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTa               >  2:482680/1‑140 (MQ=255)

                                                                                                                            cAGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAAc          <  1:482680/140‑1 (MQ=255)

                                                                                                                             aGGTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACg         <  2:77823/140‑1 (MQ=255)

                                                                                                                              ggTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACgg        >  1:372484/1‑140 (MQ=255)

                                                                                                                              ggTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACgg        >  2:733266/1‑140 (MQ=255)

                                                                                                                              ggTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACgg        >  1:518664/1‑140 (MQ=255)

                                                                                                                              ggTGATATCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACgg        >  2:811362/1‑140 (MQ=255)

                                                                                                                                    atCACTGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGaaa  >  2:719503/1‑140 (MQ=255)

CTGACCAGTTTGAAAAATGGTGATTATAGGGTTACGGCCTCTGTGAGCTCTGGTTCCCAGGCTAATCAACAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAA  >  NC_000913/2047650‑2047921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A