Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,167,586	T→C	T45A (ACG→GCG)	ygiS ←	putative deoxycholate binding periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,167,586	0	T	C	100.0%	158.1 / NA	45	T45A (ACG→GCG)	ygiS	putative deoxycholate binding periplasmic protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/23); total (22/23)

GCCGTCCAGTATCTCCCAGCGTTCAGCCTGAGCGGGCTGCACCTGGCCTTCACCGTCCATCCATACCAGACCTTCAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGTACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGCTGACCAGCCATAATAAATTTCGCGTATACA  >  NC_000913/3167452‑3167717

gccgTCCAGTATCTCCCAGCGTTCAGCCTGAGCGGGCTGCACCTGGCCTTCACCGTCCATCCATACCAGACCTTCAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTg                                                                                                                                <  2:55149/140‑1 (MQ=255)

              cccAGCGTTCAGCCTGAGCGGGCTGCACCTGGCCTTCACCGTCCATCCATACCAGACCTTCAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGAttg                                                                                                                  >  1:165949/1‑140 (MQ=255)

              cccAGCGTTCAGCCTGAGCGGGCTGCACCTGGCCTTCACCGTCCATCCATACCAGACCTTCAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGAttg                                                                                                                  >  2:40418/1‑140 (MQ=255)

                     ttCAGCCTGAGCGGGCTGCACCTGGCCTTCACCGTCCATCCATACCAGACCTTCAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAAc                                                                                                           >  1:716768/1‑140 (MQ=255)

                       cAGCCTGAGCGGGCTGCACCTGGCCTTCACCGTCCATCCATACCAGACCTTCAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGa                                                                                                         <  2:165949/140‑1 (MQ=255)

                            tGAGCGGGCTGCACCTGGCCTTCACCGTCCATCCATACCAGACCTTCAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGAc                                                                                                    <  1:176999/140‑1 (MQ=255)

                             gAGCGGGCTGCACCTGG‑CTTCACCGTCCATCCATACCAGACCTTCAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACtt                                                                                                  >  1:825363/1‑140 (MQ=255)

                                       cACCTGGCCTTCACCGTCCATCCATACCAGACCTTCAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCgggg                                                                                         <  2:716768/140‑1 (MQ=255)

                                            ggCCTTCACCGTCCATCCATACCAGACCTTCAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGGCTTGTTGCGGGGCGAGc                                                                                    >  2:769224/1‑140 (MQ=255)

                                                  cACCGTCCATCCATACCAGACCTTCAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGgtgtg                                                                              >  2:599235/1‑140 (MQ=255)

                                                        ccatccatACCAGACCTTCAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCg                                                                        <  1:5282/140‑1 (MQ=255)

                                                         catccatACCAGACCTTCAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCgg                                                                       >  2:656066/1‑140 (MQ=255)

                                                               tACCAGACCTTCAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGt                                                                 >  2:355589/1‑140 (MQ=255)

                                                                  cAGACCTTCAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTcag                                                              <  2:207473/140‑1 (MQ=255)

                                                                  cAGACCTTCAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTcag                                                              <  2:684538/140‑1 (MQ=255)

                                                                  cAGACCTTCAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTcag                                                              >  1:684538/1‑140 (MQ=255)

                                                                          cAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTag                                                      >  2:341564/1‑140 (MQ=255)

                                                                            aaCAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTagag                                                    <  2:455213/140‑1 (MQ=255)

                                                                               aGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAgagg                                                 >  2:838776/1‑140 (MQ=255)

                                                                                    cAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCg                                            <  1:100213/140‑1 (MQ=255)

                                                                                       cacaATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAgaggag                                               <  1:704521/134‑1 (MQ=255)

                                                                                       cacaATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAgaggag                                               >  2:704521/1‑134 (MQ=255)

                                                                                           aTCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTAc                                     >  1:731003/1‑140 (MQ=255)

                                                                                           aTCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTAc                                     >  1:354308/1‑140 (MQ=255)

                                                                                               gcgcGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAgg                                 <  1:769224/140‑1 (MQ=255)

                                                                                                cgcgGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGc                                >  2:853601/1‑140 (MQ=255)

                                                                                                cgcgGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGc                                >  1:623298/1‑140 (MQ=255)

                                                                                                 gcgGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTa                                      <  2:223912/133‑1 (MQ=255)

                                                                                                 gcgGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTa                                      >  1:223912/1‑133 (MQ=255)

                                                                                                             tcctccACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGCTGAc                            <  2:744502/131‑1 (MQ=255)

                                                                                                             tcctccACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGCTGAc                            >  1:744502/1‑131 (MQ=255)

                                                                                                               ctccACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGCTGACCAGCCataata                 >  2:291199/1‑140 (MQ=255)

                                                                                                               ctccACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGCTGACCAGCCataata                 >  1:605986/1‑140 (MQ=255)

                                                                                                                  cACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGGGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGCTGACCAGCCATAATAAAt              <  2:623298/140‑1 (MQ=255)

                                                                                                                  cACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGCTGACCAGCCATAATAAAt              <  2:354308/140‑1 (MQ=255)

                                                                                                                  cACCTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACAAGGCTGACCAGCCATAATAAAt              >  2:647487/1‑140 (MQ=255)

                                                                                                                    ccTTTTGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGGACGTCAGCAGCGTAAGGGGGAGCCGCACTTAACAGGCAGACCAGCCATAATAAAttt            >  2:199139/1‑140 (MQ=255)

                                                                                                                      ttttGCGGGTCGAGCGCCCCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGCTGACCAGCCATAATAAATTTcg          <  1:199139/140‑1 (MQ=255)

                                                                                                                       tttGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGCTGACCAGCCATAATAAATTTcgc         <  1:656066/140‑1 (MQ=255)

                                                                                                                       tttGCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGCTGACCAGCCATAATAAATTTcgc         <  1:291199/140‑1 (MQ=255)

                                                                                                                          gCGGGTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGCTGACCAGCCATAATAAATTTCGCGta      <  2:825363/140‑1 (MQ=255)

                                                                                                                            gggTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGCTGACCAGCCATAATAAATTTCGCGtata    <  1:599235/140‑1 (MQ=255)

                                                                                                                            gggTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGCTGACCAGCCATAATAAATTTCGCGtata    <  1:341564/140‑1 (MQ=255)

                                                                                                                              gTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGCTGACCAGCCATAATAAATTTCGCGTATACa  <  1:838776/140‑1 (MQ=255)

                                                                                                                              gTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGCTGACCAGCCATAATAAATTTCGCGTATACa  <  1:853601/140‑1 (MQ=255)

                                                                                                                              gTCGAGCGCACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGCTGACCAGCCATAATAAATTTCGCGTATACa  <  1:355589/140‑1 (MQ=255)

GCCGTCCAGTATCTCCCAGCGTTCAGCCTGAGCGGGCTGCACCTGGCCTTCACCGTCCATCCATACCAGACCTTCAAACAGATCCAGCACAATCTGCGCGGCAGTATTCTCCTCCACCTTTTGCGGGTCGAGCGTACCTGGGTCGCTATGATTGTTGTAACGAAAGACTTGTTGCGGGGCGAGCGGTGTGTTGGCGGGAACGTCAGCAGCGTAGAGAGGAGCCGCACTTACCAGGCTGACCAGCCATAATAAATTTCGCGTATACA  >  NC_000913/3167452‑3167717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A